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H. B. Beryl Rappaport

@hbrappap.bsky.social
411 followers 292 following 47 posts

PhD Candidate @ Syracuse University protists, extremophiles, & science art | she/her

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H. B. Beryl Rappaport @hbrappap.bsky.social · 24/09/2026
love the trademark
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H. B. Beryl Rappaport @hbrappap.bsky.social · 23/09/2026
thank you for the thoughtful coverage @npr.org ! really enjoyed talking with Ari & @oliverio.bsky.social #protistsonsky
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H. B. Beryl Rappaport @hbrappap.bsky.social · 23/09/2026
protistsonfire!!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 23/09/2026
thanks Julia!!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 23/09/2026
cool I hadn’t heard of that ant!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 23/09/2026
adding a note on “complex life” and capturing nuance when talking with different audiences: for the record, I do not think any form of life is simple. bacteria, archaea, & eukaryotes are complex in their own ways
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H. B. Beryl Rappaport @hbrappap.bsky.social · 23/09/2026
🦠😊
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Reposted by H. B. Beryl Rappaport
Jess Niblo @jessniblo.bsky.social · 22/09/2026
Our work showcases the power of protein language models to uncover new biology, and points at extremophile eukaryotes as an untapped source of interesting protein biophysics. We’re currently working on an expanded, openly available version of our pipeline. Reach out if interested in learning more!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 22/09/2026
🧡🔥
Incendiamoeba cascadensis cell
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H. B. Beryl Rappaport @hbrappap.bsky.social · 22/09/2026
Plus new discussion & other new analyses including from @skruberk.bsky.social & Ethan MacVicar showing surface bacterial density was associated with amoeba velocity but not with temperature!
Figure comparing bacterial surface density to temperature and amoeba motility
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H. B. Beryl Rappaport @hbrappap.bsky.social · 22/09/2026
Also a highly expanded analysis of predicted proteins from @shaharsu.bsky.social @jessniblo.bsky.social comparing the fire amoeba to other organisms and revealing potential convergent mechanisms with hyperthermophilic bacteria & archaea
Figure comparing biophysical properties of Incendiamoeba proteins to orthologs from 13 other organisms
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H. B. Beryl Rappaport @hbrappap.bsky.social · 22/09/2026
Since the preprint, we’ve added RNAseq experiments from @negilbert.bsky.social showing pathways upregulated at high temperature including proteostasis and membrane trafficking!
Figure showing upregulation vs. downregulation of gene pathways at 61 relative to 48C
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H. B. Beryl Rappaport @hbrappap.bsky.social · 22/09/2026
Our description of fire amoeba, 𝘐𝘯𝘤𝘦𝘯𝘥𝘪𝘢𝘮𝘰𝘦𝘣𝘢 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴, is now online at Cell! Thanks @oliverio.bsky.social and amazing team for questioning the limits of eukaryotes! 🔥 www.cell.com/cell/fulltex...
cell.com
A geothermal amoeba sets a new upper temperature limit for eukaryotes
The amoeba Incendiamoeba cascadensis demonstrates that eukaryotic life can withstand temperatures beyond what was thought possible and sheds light on molecular strategies for survival in extreme heat.
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H. B. Beryl Rappaport @hbrappap.bsky.social · 22/09/2026
thanks Ken!!!🦠🗻
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H. B. Beryl Rappaport @hbrappap.bsky.social · 17/09/2026
Back to Syracuse from Aotearoa-New Zealand! Thanks #ISME20 for the great extremophiles turnout and Stott Lab for bringing me along sampling - excited to learn more about the geothermal amoebae from this beautiful country!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 20/05/2026
hi #abscicon26! I’m looking forward to talking about Incendiamoeba and friends (“Microbial eukaryotic interactions in geothermal springs”) in tomorrow’s 2-3:30 Symbioses session
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Reposted by H. B. Beryl Rappaport
Rachel Shepherd 🪴 @rmshepherd.bsky.social · 27/03/2026
Undergraduate researcher extraordinaire @melpaige05.bsky.social presented some of her recent findings on the role of temperature for the three life stages of a Heterolobosean protist (Fumarolamoeba sp.) today! Melanie's passion for pink posters and characterizing this amoeba is inspiring 💓🦠
Two scientists with big smiles are standing by a scientific poster that is colored bright pink.
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Reposted by H. B. Beryl Rappaport
Julia Van Etten @couchmicroscopy.bsky.social · 14/03/2026
I have a new review out in TiG titled, “The ecology of horizontal gene transfer”! I’ve been thinking about these ideas for years and this field is growing so fast it’s becoming impossible to review it. I hope this paper sparks discussion on different ways we can look at DNA transfer!
sciencedirect.com
The ecology of horizontal gene transfer
Horizontal gene transfer (HGT) generates genetic variation in populations across all domains of life; however, most studies focus on individual transf…
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Reposted by H. B. Beryl Rappaport
Rachel Shepherd 🪴 @rmshepherd.bsky.social · 27/02/2026
Hot off the press! If you’ve ever wondered how ecosystem-engineering gopher tortoises shape soil microbial and nematode communities, this paper is for you! 🐢💚 link.springer.com/article/10.1...
link.springer.com
Gopher tortoise influence on soil biodiversity is constrained by historical land use - Biodiversity and Conservation
Keystone species and ecosystem engineers play critical roles in maintaining and enhancing biodiversity by modifying their environment and creating ecological niches that support a wide range of organi...
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Gautam Dey @gautamdey.bsky.social · 04/02/2026
Thank you @mollyherring.bsky.social for the stunning showcase 😍 Like what you see? Come ExM with us! Let’s flood the internet with microbial marvels 🦠 #ProtistsOnSky #MicroEvoSky 🧪🔬🌍 @dudinlab.bsky.social @centriolelab.bsky.social @moorefound.bsky.social @embl.org @erc.europa.eu @embo.org
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STCmicrobeblog @stcmicrobeblog.bsky.social · 02/02/2026
Some like it hot… by Christoph — ...and feel right at home in a tributary of Hot Springs Creek in LVNP (California, USA), where temperatures range between 49 °C and 65 °C You are now going to learn a little bit about the amoeba, which was sampled at these "hot spots" by Rappaport et al.….
smallthingsconsidered.blog
Some like it hot…
by Christoph — ...and feel right at home in a tributary of Hot Springs Creek in LVNP (California, USA), where temperatures range between 49 °C and 65 °C You are now going to learn a little bit about the amoeba, which was sampled at these "hot spots" by Rappaport et al.….
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Maximilian Ganser @mhganser.bsky.social · 27/12/2025
Finally, we’ve solved a long-standing mystery: what tintinnid shells are actually made of: A new class of biomaterial formed by remarkable structural proteins unique to tintinnids. A major milestone after 3 years of work! Read about it in our preprint: doi.org/10.64898/202... #ProtistsOnSky
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H. B. Beryl Rappaport @hbrappap.bsky.social · 22/12/2025
Thanks Julia!!
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angela oliverio @oliverio.bsky.social · 18/12/2025
"A newly identified amoeba can survive at the temperature of a medium-well done steak" lol @scifri.bsky.social an apt comparison a hadn't previously considered... A pleasure chatting about our research led by @hbrappap.bsky.social & with a wonderful team of colleagues.
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Ocean Hoptimism @oceanhoptimism.org · 06/12/2025
Sometimes life finds a way… like this newly discovered “fire amoeba,” thriving at 145 °F deep in the hot springs of Lassen Volcanic National Park 🌋 Even in the harshest places, life endures. That kind of resilience gives us hope. ✨ #OceanHoptimism #NatureFindsAWay #Extremophiles #Resilience
sfgate.com
Scientists find a startling discovery in a national park's hot waters
Scientists found a new organism that thrives in a national park's hot waters.
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angela oliverio @oliverio.bsky.social · 05/12/2025
Delighted to share some coverage of our new preprint (doi.org/10.1101/2025...) from Scientific American and thank you @sciam.bsky.social @andreatweather.bsky.social for taking the time to chat with @hbrappap.bsky.social and I about why we are so excited about the geothermal 𝘐𝘯𝘤𝘦𝘯𝘥𝘪𝘢𝘮𝘰𝘦𝘣𝘢
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H. B. Beryl Rappaport @hbrappap.bsky.social · 02/12/2025
Yes samples were kept at ambient temps so likely we are missing out on any thermophiles that can’t encyst at lower temps - hoping at some point to sequence samples preserved in the field in part because of this!
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angela oliverio @oliverio.bsky.social · 02/12/2025
Thanks nature news for featuring our recent preprint on 𝘐𝘯𝘤𝘦𝘯𝘥𝘪𝘢𝘮𝘰𝘦𝘣𝘢 led by @hbrappap.bsky.social (check out preprint here: doi.org/10.1101/2025...) #protistsonsky
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H. B. Beryl Rappaport @hbrappap.bsky.social · 01/12/2025
Awesome, that is good with us :)
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H. B. Beryl Rappaport @hbrappap.bsky.social · 01/12/2025
Yes, that’s the place! Cool, interested in what you’ve found there
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H. B. Beryl Rappaport @hbrappap.bsky.social · 01/12/2025
thanks for these musings!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 28/11/2025
yes we just have multiple filamentous bacteria in the culture, but M. ruber is definitely there and seems to be most abundant based on sequencing!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 27/11/2025
most likely M. ruber! so interesting, didn’t know that about filamentation! 🍝 & not sure why the file conversions were named that way but the order looks accurate as labeled - Supplemental Movie 1-12
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H. B. Beryl Rappaport @hbrappap.bsky.social · 27/11/2025
the long thin guys are filamentous bacteria and the clump is moving oddly but I believe it’s just bacterial biofilm
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H. B. Beryl Rappaport @hbrappap.bsky.social · 27/11/2025
so awesome!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 26/11/2025
Tbd! We need to redo some staining
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H. B. Beryl Rappaport @hbrappap.bsky.social · 26/11/2025
thermophilic bacteria!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 26/11/2025
I believe within their tubes they can withstand higher temps but the worms themselves max out around 55°C, and temps for reproduction and early dev may be lower - their max tolerance is super interesting though! pmc.ncbi.nlm.nih.gov/articles/PMC...
pmc.ncbi.nlm.nih.gov
Thermal Limit for Metazoan Life in Question: In Vivo Heat Tolerance of the Pompeii Worm
The thermal limit for metazoan life, expected to be around 50°C, has been debated since the discovery of the Pompeii worm Alvinella pompejana, which colonizes black smoker chimney walls at deep-sea vents. While indirect evidence predicts body ...
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H. B. Beryl Rappaport @hbrappap.bsky.social · 26/11/2025
We are looking into this 👀
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
Yes, these movies can be downloaded from the supp materials here! doi.org/10.1101/2025...
doi.org
A geothermal amoeba sets a new upper temperature limit for eukaryotes
The study of temperature limits has transformed our knowledge of the boundaries of life but has been largely focused on bacteria and archaea. We isolated a novel geothermal amoeba, Incendiamoeba casca...
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
We will have all sequences up on NCBI with publication for you to take a look! Also really interested in their chaperone network!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
And finally all at SyracuseU - @shaharsu.bsky.social @jessniblo.bsky.social for protein analyses, @rmshepherd.bsky.social for culturing, & @oliverio.bsky.social for leading this effort with me!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
@xtremevirusprof.bsky.social @nachopus.bsky.social and team at PSU and Gordon Wolfe at Chico for sampling and extremophile expertise @lauraakatz.bsky.social and Godwin Ani at Smith for phylogenomics
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
@fmschu.bsky.social New Lineages of Life group at JGI with Tomas Tyml for culturing, seq prep, & biogeography, and Kurt LaButti also at LBNL for bioinformatics @fmikus.bsky.social @dudinlab.bsky.social at Geneva and @gautamdey.bsky.social at EMBL for beautiful U-ExM
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
So grateful to all collaborators on this huge effort! @mullinslab.bsky.social at UCSF with Natalie Petek, @samjlord.bsky.social, and @skruberk.bsky.social now at Gonzaga with Ethan MacVicar for microscopy & motility analyses
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
𝘐. 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 makes us think about which factors set the upper temp limit for euks and what the physical limit is - are there relatives that grow even hotter? How do euks adapt to extreme conditions? 🌋🦠
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
We assembled the genome of 𝘐. 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 and found enriched genes that may be associated with thermophily - many involved in signaling and protein stability. 𝘐. 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 proteins also have higher predicted melting temps and higher net surface charges than 𝘝. 𝘷𝘦𝘳𝘮𝘪𝘧𝘰𝘳𝘮𝘪𝘴.
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
We tracked the motility of 𝘐. 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 across temp and found it is active up to 64°C (movie here), with most directed motion from 55-64°C🏃‍♀️! It seems to use the vermiform state to travel quickly and the amoebiform state to explore space.
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
While 𝘝. 𝘷𝘦𝘳𝘮𝘪𝘧𝘰𝘳𝘮𝘪𝘴 max temp is around 45°C, we found 𝘐. 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 grows up to 63°C with optimal growth at 55-57°C🥵. We showed cellular replication via Ultrastructure Expansion Microscopy at 63°C. The previous limit for euks is 60°C!
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H. B. Beryl Rappaport @hbrappap.bsky.social · 25/11/2025
Using phylogenomics, we placed the amoeba as a novel genus within #Amoebozoa, which we named 𝘐𝘯𝘤𝘦𝘯𝘥𝘪𝘢𝘮𝘰𝘦𝘣𝘢 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 (left). Its sister is 𝘝𝘦𝘳𝘮𝘢𝘮𝘰𝘦𝘣𝘢 𝘷𝘦𝘳𝘮𝘪𝘧𝘰𝘳𝘮𝘪𝘴 (right) - both have a “vermiform” and “amoebiform” state.
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