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Christian Gallardo

@gallardo-seq.bsky.social
1.2K followers 1.2K following 31 posts

Long read sequencing of RNA viruses and their hosts | RNA structure-function | Genomics technology development | Genomics assay R&D | Scripps Research 🎓 | 🇦🇷 🇺🇸 | Senior Scientist at @TwistBioscience.com

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Christian Gallardo @gallardo-seq.bsky.social · 09/02/2026
Well done Seahawks! Seattle must be quite the party tonight!
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Phil Lewis @phillewis.bsky.social · 09/02/2026
‼️
This is incredibly cool — A source familiar with Bad Bunny’s performance tonight tells me that the couple in the halftime show got married for real.

They invited Bad Bunny to their wedding and in turn he invited them to get married during his performance.
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Soraya Nadia McDonald @soraya.press · 09/02/2026
Benito is a threat b/c he makes art so alluring and enjoyable you want to understand everything about it and then you end up learning about sugar and slavery and colonialism and the Taínos and Hawaii and then you probably have some thoughts of your own, and that's why art is powerful and dangerous
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Alexandria Ocasio-Cortez @aoc.bsky.social · 09/02/2026
Ocasio gang rise up 🙌🏽🇵🇷
Bad Bunny wearing an OCASIO 64 jersey
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Kevin Gannon (now a Moo Deng fan account) @thetattooedprof.bsky.social · 09/02/2026
OK "god bless america" and then naming every country in the americas from south to north is absolute king shit
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Christian Gallardo @gallardo-seq.bsky.social · 09/02/2026
I’m re-emerging from a long lull to just say that Bad Bunny Super Tazón Show was a cultural moment in Latinidad.
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Christian Gallardo @gallardo-seq.bsky.social · 21/12/2025
What a guy…
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Rick Steves @ricksteves.bsky.social · 21/12/2025
It’s the “season of love and giving”…but this year, doesn’t it seem more like a “season of fear and taking”? Like many of you, I’ve been saddened by the human impact of draconian government budget cuts and how angry many housed Americans are at unhoused Americans. 🧵 1 of 9
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Dr. Stephanie @punkrockscience.bsky.social · 29/08/2025
I was thinking about this in another thread, but - what *are* the details that would make a show set in a bio/chem lab “realistic”? - the pipette always needs a tip when used - some things are *scrupulously* clean, to the point that they’re worn from it - lab coats need to be dirty (or disposable)
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Harmit Singh Malik @harmitmalik.bsky.social · 23/08/2025
It bears repeating that nearly the entire US enterprise in science and technology has been irreversibly gutted. This is shocking. All this loss has occurred without any real benefit to the average US citizen. Except we now have the biggest, best funded secret police force perhaps ever. Yay for us!
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Sebastian S. Cocioba @atinygreencell.bsky.social · 23/08/2025
Catching some falling stars
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Debnath Ghosal @debnathghosal.bsky.social · 02/08/2025
📣 New paper alert! Just out in Cell Reports! pubmed.ncbi.nlm.nih.gov/40644298/ Thrilled to share that we have discovered a brand-new anti-phage defense system! Bacteria have evolved various defense strategies (CRISPR etc) to counter phage attacks. We found a new one - fascinating and dramatic ⚔️🦠❄️🔬
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Twist Bioscience @twistbioscience.com · 07/07/2025
💥 Boom! All gene fragment formats starting at 2 day turnaround time, only 7¢ per base. Press Release: investors.twistbioscience.com/news-release... @twistbioscience.com
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Gregg Gonsalves @gregggonsalves.bsky.social · 08/05/2025
Who are the functionaries carrying out the destruction of the NIH? The Gang of Four: Memoli, McElroy, Schnabel, Bhattacharya. There are others, but these men play major day-to-day roles, they are the implementers, the enforcers, the true believers. www.thenation.com/article/poli...
thenation.com
Inside the Bloodbath at the NIH
Sources say that a climate of fear has spread throughout the agency as the Trump administration takes a hatchet to its core functions.
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jenarna.bsky.social @jenarna.bsky.social · 28/04/2025
Check out our recently published paper about identifying RNA modifications from raw Nanopore data: www.nature.com/articles/s42... Congrats to Jannes, Manja and all the co-authors!
nature.com
The RMaP challenge of predicting RNA modifications by nanopore sequencing - Communications Chemistry
RNA modifications play critical roles in gene expression regulation, RNA stability, and translation efficiency, however, the detection of RNA modifications by various sequencing approaches may present...
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Eugene F. Baulin @febos.bsky.social · 27/04/2025
Couldn’t wait to share - SQUARNA 2.0 is here, with greatly improved performance in single-sequence RNA secondary structure prediction! github.com/febos/SQUARNA Updated preprint is coming soon :) #SQUARNA #RNA #2D #Structure #Prediction #Bioinformatics #Research #Pseudoknot
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Vaughn Cooper @vscooper.micropopbio.org · 28/04/2025
Important 🧵 if your research depends on genome assemblies from long (PacBio / ONT) reads. As @merenbey.bsky.social says, these may not be the genomes you are looking for. TLDR: the assemblers are wrong and predict too many small circular contigs.
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Nucleic Acids Research @narjournal.bsky.social · 25/04/2025
🧬 Happy National DNA Day! 🧬 Today we celebrate the completion of the human genome project (2003) and discovery of the double helix (1953). From genome editing to RNA therapeutics, research into nucleic acids continues to drive scientific breakthroughs #NationalDNADay #NucleicAcids #DNAday2025
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Stuart Neil @tetherin.bsky.social · 22/04/2025
The Palantirs were originally made so that the Men of the West could communicate. But then they were taken over by the Enemy to spread corruption, sow fear, and spread disinformation to destroy the world of Men. Kudos to Peter Thiel for choosing the most accurate name for his company.
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Niko Bowie @nikobowie.bsky.social · 14/04/2025
BREAKING: On Friday, the federal government issued new demands of Harvard University. The university's lawyers just responded: back off.
April 14, 2025
VIA ELECTRONIC MAIL
Josh Gruenbaum
Commissioner of the Federal Acquisition Service
General Services Administration
Sean R. Keveney
Acting General Counsel
U.S. Department of Health & Human Services
Thomas E. Wheeler
Acting General Counsel
U.S. Department of Education
Dear Messrs. Gruenbaum, Keveney, and Wheeler:
We represent Harvard University. We are writing in response to your letter dated April 11,
2025, addressed to Dr. Alan Garber, Harvard’s President, and Penny Pritzker, Senior Fellow of the
Harvard Corporation.
Harvard is committed to fighting antisemitism and other forms of bigotry in its community.
Antisemitism and discrimination of any kind not only are abhorrent and antithetical to Harvard’s
values but also threaten its academic mission.
To that end, Harvard has made, and will continue to make, lasting and robust structural,
policy, and programmatic changes to ensure that the university is a welcoming and supportive
learning environment for all students and continues to abide in all respects with federal law across
its academic programs and operations, while fostering open inquiry in a pluralistic community free
from intimidation and open to challenging orthodoxies, whatever their source.
Over the past 15 months, Harvard has undertaken substantial policy and programmatic
measures. It has made changes to its campus use policies; adopted new accountability procedures;
imposed meaningful discipline for those who violate university policies; enhanced programs
designed to address bias and promote ideological diversity and civil discourse; hired staff to
support these programs and support students; changed partnerships; dedicated resources to combat
hate and bias; and enhanced safety and security measures. As a result, Harvard is in a very different
place today from where it was a year ago. These efforts, and additional measures the university
will be taking against antisemitism, not only are the right thing to do but also are critical to
strength…recognized by the Supreme Court. The government’s terms also circumvent Harvard’s statutory
rights by requiring unsupported and disruptive remedies for alleged harms that the government has
not proven through mandatory processes established by Congress and required by law. No less
objectionable is the condition, first made explicit in the letter of March 31, 2025, that Harvard
accede to these terms or risk the loss of billions of dollars in federal funding critical to vital research
and innovation that has saved and improved lives and allowed Harvard to play a central role in
making our country’s scientific, medical, and other research communities the standard-bearers for
the world. These demands extend not only to Harvard but to separately incorporated and
independently operated medical and research hospitals engaging in life-saving work on behalf of
their patients. The university will not surrender its independence or relinquish its constitutional
rights. Neither Harvard nor any other private university can allow itself to be taken over by the
federal government. Accordingly, Harvard will not accept the government’s terms as an agreement
in principle.
Harvard remains open to dialogue about what the university has done, and is planning to
do, to improve the experience of every member of its community. But Harvard is not prepared to
agree to demands that go beyond the lawful authority of this or any administration.
William A. Burck Robert K. Hur
Quinn Emanuel Urquhart & Sullivan, LLP 1300 I Street NW Suite 900 Suite 900
Washington, DC 20005 King & Spalding LLP
1700 Pennsylvania Avenue NW
Washington, DC 20006
11446411105
Christian Gallardo @gallardo-seq.bsky.social · 10/04/2025
This is not a drill…
theguardian.com
We are witnessing the destruction of science in America | Paul Darren Bieniasz
If we stay on this administration’s course, future life-saving medicines may never be invented
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Olga Nesterova @onestpress.onestnetwork.com · 08/04/2025
Leavitt confirmed that the WH is working on deporting US citizens to El Salvador
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Heng Li @lh3lh3.bsky.social · 24/03/2025
longcallD is a new variant caller for genomic long reads. It jointly calls phased small and structural variants. Single binary, one command line for the whole process. Comparable accuracy to mainstream callers. Great work by Yan Gao. github.com/yangao07/lon...
github.com
GitHub - yangao07/longcallD: A local-haplotagging-based small and structural variant caller
A local-haplotagging-based small and structural variant caller - yangao07/longcallD
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Harmit Singh Malik @harmitmalik.bsky.social · 14/03/2025
When the occasion demands the Machiavellianism of a McConnell but all you have to offer is the ‘make nice’ of a Schumer Like I’ve said before… Disney characters who don’t know they’re trapped in a Tarantino film
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Alex Wild @alexwild.bsky.social · 13/03/2025
This is the formal end of the Democratic Party on the national stage. There is no constituency for an opposition party that doesn’t oppose.
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Chris Hayes @chrislhayes.bsky.social · 13/03/2025
The institutional Democratic Party is guided by an almost pathological level of conflict avoidance in almost every direction. “What can we do to make the least number of people mad?” is just a bankrupt way to operate.
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Jonathan Cohn @jonathancohn.bsky.social · 13/03/2025
Where is the lie? theonion.com/democrats-si...
theonion.com
Democrats Sick Of Being Blamed For Cowardice On Issues They Actually Just Don’t Care About
WASHINGTON—Having thus far caved on eliminating the filibuster, advancing an adequate climate change agenda, and protecting voting rights, congressional Democrats told reporters Wednesday they were si...
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Drug Monkey @drugmonkey.bsky.social · 28/02/2025
“You aren’t kissing Dear Leader’s ass enough” is a helluva foreign policy.
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Sam Bagenstos @sbagen.bsky.social · 27/02/2025
Read every word of this article to see what the Trump folks are doing to the good civil servants at NIH -- and to the important work that protects all of our health. www.theatlantic.com/health/archi...
theatlantic.com
Inside the Collapse at NIH
Administration officials pressured NIH to avoid clear advice from the agency’s own lawyers to restart grant funding now.
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Ben Collins @bencollins.bsky.social · 26/02/2025
Five alarm fire. www.politico.com/news/2025/02...
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Randall Munroe @xkcd.com · 26/02/2025
RNA xkcd.com/3056
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Harmit Singh Malik @harmitmalik.bsky.social · 20/02/2025
Biomedical graduate admissions paused or cut back as some universities respond to Trump orders on research - STAT apple.news/APlQhGHRrSfy...
apple.news
Graduate student admissions paused and cut back as universities react to Trump orders on research — STAT
Acceptances for biomedical graduate students and postdoctoral scholars are being cut back at some universities and medical centers across the country as many grapple with the potential impact of the T...
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Christian Gallardo @gallardo-seq.bsky.social · 03/02/2025
Sickening how the mainstream media and our elected representatives have been so passive about this constitutional crisis.
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Christian Gallardo @gallardo-seq.bsky.social · 02/02/2025
and unconstitutional…
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Rex Huppke @rexhuppke.bsky.social · 30/01/2025
Ah. Clearly DEI mandates led to the hiring of invisible people.
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Christian Gallardo @gallardo-seq.bsky.social · 22/01/2025
how it feels to be working on RPPRs right now.
"This if Fine" meme with caption "Working on my RPPRs"
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Christian Gallardo @gallardo-seq.bsky.social · 22/01/2025
***Study Sections (and Advisory Councils) scheduled between now and Feb 2nd*** Extremely alarming nonetheless, but let's make sure people have accurate info.
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Keith Robison @omicsomics.bsky.social · 14/01/2025
H/t to Nava for spotting this & posting to ASeq discord First question posted to @nanopore CEO Gordon Sanghera at #JPM25 had 2 parts: 1. Do they have long read technology? 2. Do they see value in long reads?
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Christian Gallardo @gallardo-seq.bsky.social · 21/12/2024
🤯
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Lena Steckelberg @lenasteckelberg.bsky.social · 20/12/2024
Hi Bluesky, just in time for the holidays I am excited to share the latest pre-print from my group! We solved the 3D structure of a mysterious viral RNA that resists degradation by host nucleases. A short 🧵 &link below – please also check out the full video! #RNA #RNAbiology #RNASky #lovevirology
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Angie Rasmussen @angierasmussen.bsky.social · 18/12/2024
My replies are perpetually full of anti-vaxxers these days telling me about polio vaccines. Not shockingly, most of what they are saying is wrong. Luckily, I trained with Vincent Racaniello & he taught me a few things about poliovirus. So let’s discuss the king of the Picornaviridae👇🏻
Picture of a plaque assay (purple field with clear holes) showing gorgeous poliovirus plaques
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Jim Shaw @jimshaw.bsky.social · 17/12/2024
In a nutshell, devider can cluster long reads into similar haplotypes (see the images). Main points: (1) Works best with sequence length ~= read length (2) Does not require prior knowledge of # of haplotypes (can be many!) (3) Extremely fast (> 20,000x coverage is ok!) (4) Requires reference 2/5
Heterogeneous long-read sequences due to a mixture of sequenceslong reads grouped into haplotype-concordant clusters
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Ryan Wick @rrwick.bsky.social · 16/12/2024
Just had my first experience with CycloneSEQ data and shared my findings in this blog post: rrwick.github.io/2024/12/17/c... How does CycloneSEQ compare to @nanoporetech.com? I looked at both read-level and consensus-level accuracy. Check it out!
rrwick.github.io
A first look at CycloneSEQ data
a blog for miscellaneous bioinformatics stuff
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Andreas De Block @andreasdeblock.bsky.social · 02/12/2024
Research thrives on innovation and the diversity of ideas, yet our funding system often penalizes both. In our new study published in PNAS, we explore the hidden costs of competition in allocating scarce research funds. (1/10) www.pnas.org/doi/10.1073/...
pnas.org
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
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xiuqichen.bsky.social @xiuqichen.bsky.social · 29/11/2024
Reply below if you want to be included in the #nanopore starter pack! go.bsky.app/VCoo6T
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🧬Jacob L Steenwyk @jlsteenwyk.bsky.social · 25/11/2024
TechBio Transformers (founded by @dr-alphalyrae.bsky.social) is a growing global community of folks who want to (+)ly impact the field of technology & biology, while also intentionally building healthy dialogue and community Follow along, & even consider joining the group! bsky.app/starter-pack...
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Christian Gallardo @gallardo-seq.bsky.social · 25/11/2024
It’s great to see this thorough validation of DMS chemical probing of RNA structure. I think the DMS reactivity “continuum” issue and the interplay with sequence context issue can be addressed with new ML models combined with 4-base sensitive DMS.
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Manolis Maragkakis @manolis-maragkakis.bsky.social · 24/11/2024
RNAkinet identifies nascent RNA by predicting 5EU metabolically labeled #RNA from raw #nanopore electrical signal. Should allow association of RNA metabolism dynamics with isoform usage, poly(A) tails and native RNA mods. academic.oup.com/nargab/artic...
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