Christian Gallardo @gallardo-seq.bsky.social · 09/02/2026Well done Seahawks! Seattle must be quite the party tonight! 020
Reposted by Christian GallardoSoraya Nadia McDonald @soraya.press · 09/02/2026Benito is a threat b/c he makes art so alluring and enjoyable you want to understand everything about it and then you end up learning about sugar and slavery and colonialism and the Taínos and Hawaii and then you probably have some thoughts of your own, and that's why art is powerful and dangerous 106204615190
Reposted by Christian GallardoAlexandria Ocasio-Cortez @aoc.bsky.social · 09/02/2026Ocasio gang rise up 🙌🏽🇵🇷 475463163760
Reposted by Christian GallardoKevin Gannon (now a Moo Deng fan account) @thetattooedprof.bsky.social · 09/02/2026OK "god bless america" and then naming every country in the americas from south to north is absolute king shit 174286704953
Christian Gallardo @gallardo-seq.bsky.social · 09/02/2026I’m re-emerging from a long lull to just say that Bad Bunny Super Tazón Show was a cultural moment in Latinidad. 010
Reposted by Christian GallardoRick Steves @ricksteves.bsky.social · 21/12/2025It’s the “season of love and giving”…but this year, doesn’t it seem more like a “season of fear and taking”? Like many of you, I’ve been saddened by the human impact of draconian government budget cuts and how angry many housed Americans are at unhoused Americans. 🧵 1 of 9 818228245908
Reposted by Christian GallardoDr. Stephanie @punkrockscience.bsky.social · 29/08/2025I was thinking about this in another thread, but - what *are* the details that would make a show set in a bio/chem lab “realistic”? - the pipette always needs a tip when used - some things are *scrupulously* clean, to the point that they’re worn from it - lab coats need to be dirty (or disposable) 209719
Reposted by Christian GallardoHarmit Singh Malik @harmitmalik.bsky.social · 23/08/2025It bears repeating that nearly the entire US enterprise in science and technology has been irreversibly gutted. This is shocking. All this loss has occurred without any real benefit to the average US citizen. Except we now have the biggest, best funded secret police force perhaps ever. Yay for us! 7328118
Reposted by Christian GallardoSebastian S. Cocioba @atinygreencell.bsky.social · 23/08/2025Catching some falling stars 4578
Reposted by Christian GallardoDebnath Ghosal @debnathghosal.bsky.social · 02/08/2025📣 New paper alert! Just out in Cell Reports! pubmed.ncbi.nlm.nih.gov/40644298/ Thrilled to share that we have discovered a brand-new anti-phage defense system! Bacteria have evolved various defense strategies (CRISPR etc) to counter phage attacks. We found a new one - fascinating and dramatic ⚔️🦠❄️🔬 315153
Reposted by Christian GallardoTwist Bioscience @twistbioscience.com · 07/07/2025💥 Boom! All gene fragment formats starting at 2 day turnaround time, only 7¢ per base. Press Release: investors.twistbioscience.com/news-release... @twistbioscience.com 011
Reposted by Christian GallardoGregg Gonsalves @gregggonsalves.bsky.social · 08/05/2025Who are the functionaries carrying out the destruction of the NIH? The Gang of Four: Memoli, McElroy, Schnabel, Bhattacharya. There are others, but these men play major day-to-day roles, they are the implementers, the enforcers, the true believers. www.thenation.com/article/poli...thenation.comInside the Bloodbath at the NIHSources say that a climate of fear has spread throughout the agency as the Trump administration takes a hatchet to its core functions. 717187
Reposted by Christian Gallardojenarna.bsky.social @jenarna.bsky.social · 28/04/2025Check out our recently published paper about identifying RNA modifications from raw Nanopore data: www.nature.com/articles/s42... Congrats to Jannes, Manja and all the co-authors!nature.comThe RMaP challenge of predicting RNA modifications by nanopore sequencing - Communications ChemistryRNA modifications play critical roles in gene expression regulation, RNA stability, and translation efficiency, however, the detection of RNA modifications by various sequencing approaches may present... 032
Reposted by Christian GallardoEugene F. Baulin @febos.bsky.social · 27/04/2025Couldn’t wait to share - SQUARNA 2.0 is here, with greatly improved performance in single-sequence RNA secondary structure prediction! github.com/febos/SQUARNA Updated preprint is coming soon :) #SQUARNA #RNA #2D #Structure #Prediction #Bioinformatics #Research #Pseudoknot 041
Reposted by Christian GallardoVaughn Cooper @vscooper.micropopbio.org · 28/04/2025Important 🧵 if your research depends on genome assemblies from long (PacBio / ONT) reads. As @merenbey.bsky.social says, these may not be the genomes you are looking for. TLDR: the assemblers are wrong and predict too many small circular contigs. 12812
Reposted by Christian GallardoNucleic Acids Research @narjournal.bsky.social · 25/04/2025🧬 Happy National DNA Day! 🧬 Today we celebrate the completion of the human genome project (2003) and discovery of the double helix (1953). From genome editing to RNA therapeutics, research into nucleic acids continues to drive scientific breakthroughs #NationalDNADay #NucleicAcids #DNAday2025 1113
Reposted by Christian GallardoStuart Neil @tetherin.bsky.social · 22/04/2025The Palantirs were originally made so that the Men of the West could communicate. But then they were taken over by the Enemy to spread corruption, sow fear, and spread disinformation to destroy the world of Men. Kudos to Peter Thiel for choosing the most accurate name for his company. 183
Reposted by Christian GallardoNiko Bowie @nikobowie.bsky.social · 14/04/2025BREAKING: On Friday, the federal government issued new demands of Harvard University. The university's lawyers just responded: back off. 11446411105
Christian Gallardo @gallardo-seq.bsky.social · 10/04/2025This is not a drill…theguardian.comWe are witnessing the destruction of science in America | Paul Darren BieniaszIf we stay on this administration’s course, future life-saving medicines may never be invented 010
Reposted by Christian GallardoOlga Nesterova @onestpress.onestnetwork.com · 08/04/2025Leavitt confirmed that the WH is working on deporting US citizens to El Salvador 206390304595
Reposted by Christian GallardoHeng Li @lh3lh3.bsky.social · 24/03/2025longcallD is a new variant caller for genomic long reads. It jointly calls phased small and structural variants. Single binary, one command line for the whole process. Comparable accuracy to mainstream callers. Great work by Yan Gao. github.com/yangao07/lon...github.comGitHub - yangao07/longcallD: A local-haplotagging-based small and structural variant callerA local-haplotagging-based small and structural variant caller - yangao07/longcallD 310449
Reposted by Christian GallardoHarmit Singh Malik @harmitmalik.bsky.social · 14/03/2025When the occasion demands the Machiavellianism of a McConnell but all you have to offer is the ‘make nice’ of a Schumer Like I’ve said before… Disney characters who don’t know they’re trapped in a Tarantino film 216840
Reposted by Christian GallardoAlex Wild @alexwild.bsky.social · 13/03/2025This is the formal end of the Democratic Party on the national stage. There is no constituency for an opposition party that doesn’t oppose. 712630
Reposted by Christian GallardoChris Hayes @chrislhayes.bsky.social · 13/03/2025The institutional Democratic Party is guided by an almost pathological level of conflict avoidance in almost every direction. “What can we do to make the least number of people mad?” is just a bankrupt way to operate. 34507811615085
Reposted by Christian GallardoJonathan Cohn @jonathancohn.bsky.social · 13/03/2025Where is the lie? theonion.com/democrats-si...theonion.comDemocrats Sick Of Being Blamed For Cowardice On Issues They Actually Just Don’t Care AboutWASHINGTON—Having thus far caved on eliminating the filibuster, advancing an adequate climate change agenda, and protecting voting rights, congressional Democrats told reporters Wednesday they were si... 211823
Reposted by Christian GallardoDrug Monkey @drugmonkey.bsky.social · 28/02/2025“You aren’t kissing Dear Leader’s ass enough” is a helluva foreign policy. 1323
Reposted by Christian GallardoSam Bagenstos @sbagen.bsky.social · 27/02/2025Read every word of this article to see what the Trump folks are doing to the good civil servants at NIH -- and to the important work that protects all of our health. www.theatlantic.com/health/archi...theatlantic.comInside the Collapse at NIHAdministration officials pressured NIH to avoid clear advice from the agency’s own lawyers to restart grant funding now. 811477
Reposted by Christian GallardoBen Collins @bencollins.bsky.social · 26/02/2025Five alarm fire. www.politico.com/news/2025/02... 701115183609
Reposted by Christian GallardoHarmit Singh Malik @harmitmalik.bsky.social · 20/02/2025Biomedical graduate admissions paused or cut back as some universities respond to Trump orders on research - STAT apple.news/APlQhGHRrSfy...apple.newsGraduate student admissions paused and cut back as universities react to Trump orders on research — STATAcceptances for biomedical graduate students and postdoctoral scholars are being cut back at some universities and medical centers across the country as many grapple with the potential impact of the T... 04021
Christian Gallardo @gallardo-seq.bsky.social · 03/02/2025Sickening how the mainstream media and our elected representatives have been so passive about this constitutional crisis. 020
Reposted by Christian GallardoRex Huppke @rexhuppke.bsky.social · 30/01/2025Ah. Clearly DEI mandates led to the hiring of invisible people. 710718
Christian Gallardo @gallardo-seq.bsky.social · 22/01/2025how it feels to be working on RPPRs right now. 030
Christian Gallardo @gallardo-seq.bsky.social · 22/01/2025***Study Sections (and Advisory Councils) scheduled between now and Feb 2nd*** Extremely alarming nonetheless, but let's make sure people have accurate info. 232
Reposted by Christian GallardoKeith Robison @omicsomics.bsky.social · 14/01/2025H/t to Nava for spotting this & posting to ASeq discord First question posted to @nanopore CEO Gordon Sanghera at #JPM25 had 2 parts: 1. Do they have long read technology? 2. Do they see value in long reads? 494
Reposted by Christian GallardoLena Steckelberg @lenasteckelberg.bsky.social · 20/12/2024Hi Bluesky, just in time for the holidays I am excited to share the latest pre-print from my group! We solved the 3D structure of a mysterious viral RNA that resists degradation by host nucleases. A short 🧵 &link below – please also check out the full video! #RNA #RNAbiology #RNASky #lovevirology 713739
Reposted by Christian GallardoAngie Rasmussen @angierasmussen.bsky.social · 18/12/2024My replies are perpetually full of anti-vaxxers these days telling me about polio vaccines. Not shockingly, most of what they are saying is wrong. Luckily, I trained with Vincent Racaniello & he taught me a few things about poliovirus. So let’s discuss the king of the Picornaviridae👇🏻 35697263
Reposted by Christian GallardoJim Shaw @jimshaw.bsky.social · 17/12/2024In a nutshell, devider can cluster long reads into similar haplotypes (see the images). Main points: (1) Works best with sequence length ~= read length (2) Does not require prior knowledge of # of haplotypes (can be many!) (3) Extremely fast (> 20,000x coverage is ok!) (4) Requires reference 2/5 101
Reposted by Christian GallardoRyan Wick @rrwick.bsky.social · 16/12/2024Just had my first experience with CycloneSEQ data and shared my findings in this blog post: rrwick.github.io/2024/12/17/c... How does CycloneSEQ compare to @nanoporetech.com? I looked at both read-level and consensus-level accuracy. Check it out!rrwick.github.ioA first look at CycloneSEQ dataa blog for miscellaneous bioinformatics stuff 0137
Reposted by Christian GallardoAndreas De Block @andreasdeblock.bsky.social · 02/12/2024Research thrives on innovation and the diversity of ideas, yet our funding system often penalizes both. In our new study published in PNAS, we explore the hidden costs of competition in allocating scarce research funds. (1/10) www.pnas.org/doi/10.1073/...pnas.orgPNASProceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans... 47532
Reposted by Christian Gallardoxiuqichen.bsky.social @xiuqichen.bsky.social · 29/11/2024Reply below if you want to be included in the #nanopore starter pack! go.bsky.app/VCoo6T 141610
Reposted by Christian Gallardo🧬Jacob L Steenwyk @jlsteenwyk.bsky.social · 25/11/2024TechBio Transformers (founded by @dr-alphalyrae.bsky.social) is a growing global community of folks who want to (+)ly impact the field of technology & biology, while also intentionally building healthy dialogue and community Follow along, & even consider joining the group! bsky.app/starter-pack... 2269
Christian Gallardo @gallardo-seq.bsky.social · 25/11/2024It’s great to see this thorough validation of DMS chemical probing of RNA structure. I think the DMS reactivity “continuum” issue and the interplay with sequence context issue can be addressed with new ML models combined with 4-base sensitive DMS. 140
Reposted by Christian GallardoManolis Maragkakis @manolis-maragkakis.bsky.social · 24/11/2024RNAkinet identifies nascent RNA by predicting 5EU metabolically labeled #RNA from raw #nanopore electrical signal. Should allow association of RNA metabolism dynamics with isoform usage, poly(A) tails and native RNA mods. academic.oup.com/nargab/artic... 24512