Single-cell patch-proteomics is a novel and powerful technique combining electrophysiological and quantitative proteomic characterization from individually sampled neurons. Following whole-cell patch clamp characterization of neuronal excitability, we collect intracellular contents and, using the latest advancements in Liquid Chromatography and Mass Spectrometry, identify and quantify peptides and their corresponding proteins. By cell-matched one-to-one associations between electrophysiological properties and protein abundance, this approach provides a means to elucidate molecular mechanisms of neuronal function on individual neuron basis.

Nature Ecology & Evolution - A search for homologues of key vertebrate immune proteins in cnidarians reveals that a classic antiviral signalling pathway instead restricts antiviral activity to...

Welcome to preLights, the preprint highlights service run by the biological community and supported by The Company of Biologists. Here, a team of scientists regularly review, highlight and comment on ...

Background Genetic data have long been applied in conservation and management for threatened and endangered species to track effects of inbreeding, hybridization, and population structure. However, wh...

Trout Granulomatous Virus (TGV) is an emerging viral pathogen associated with chronic infections in adult farmed rainbow trout (Oncorhynchus mykiss). TGV infection was first described to be associated...

Infectious Hematopoietic Necrosis Virus (IHNV) is a significant economic threat to salmonid aquaculture worldwide. Understanding the factors that infl…

Anti-vax nonsense has infiltrated every corner of America’s public health agencies.

Rhinoviruses are the most frequent cause of common colds and also a major cause of respiratory distress in high-risk groups. Using single-cell sequencing of rhinovirus-infected nasal epithelial organo...

Fred Hutch is dedicated to the elimination of cancer and related diseases as causes of human suffering and death.

Several studies have described immune responses in the teleost brain and meninges during infection, however, fundamental studies that systematically d…

Teleost fish are the most diverse of the vertebrate taxa, made up of over 30,000 different species with diverse life histories, unique immune systems and extreme physiological adaptations. Mammalian i...

The NIH is “reducing animal use in research.” But there is no replacement for animal models.

Rainbow trout with depleted secretory IgM reveal interesting insights into the elusive role of this immunoglobulin in maintaining gut microbiota.