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Reposted by Daniel Jost - Physical Biology of Chromatin group

Nazlı Akıllı @nazliakilli.bsky.social · 23/09/2026

Thrilled to share my PhD paper showing how SUMO regulates PRC1 clustering and 3D genome organisation independently of H3K27me3! Grateful to @djost-physbiol.bsky.social group and the Fabian Erdel group for this great collaboration. doi.org/10.1093/nar/...

doi.org

Loss of SUMOylation drives aberrant PRC1 clustering and 3D genome rewiring independent of H3K27me3

Abstract. Polycomb repressive complex 1 (PRC1) forms nuclear condensates that organize target chromatin domains. SUMOylation modulates PRC1 clustering, but

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 21/09/2026

Look at this masterpiece from @aurelepiazza.bsky.social lab. Happy to have been part of this story. Congrats to all the team !

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 21/08/2026

🚨PRC1 nanoglobules organize Hox chromatin during Drosophila embryogenesis. New work from Cavalli lab led by Thierry Cheutin, where we performed modeling. Happy to have been part of this great work! Look at the article recently published in Cell Discovery 👇 doi.org/10.1038/s414...

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Reposted by Daniel Jost - Physical Biology of Chromatin group

Ariel Kaplan @arielkaplan.bsky.social · 06/05/2026

Happy to share our new review! Chromatin mechanics and regulatory protein function: insights from single-molecule force spectroscopy www.sciencedirect.com/science/arti...

sciencedirect.com

Chromatin mechanics and regulatory protein function: insights from single-molecule force spectroscopy

Single-molecule force spectroscopy probes chromatin mechanics by resolving force-induced conformational transitions across multiple length scales. Rec…

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Reposted by Daniel Jost - Physical Biology of Chromatin group

Romain Koszul @rkoszul.bsky.social · 16/04/2026

Happy to share our review of the past, recent, and future applications of synthetic genomics in studying the 3D functional organization of chromosomes, a set of approaches that are gaining momentum! www.sciencedirect.com/science/arti...

sciencedirect.com

Synthetic chromosomes for 3D functional genomics: from principles to AI-guided design

The role of genome 3D organization for fundamental chromatin processes, such as long-range promoter-activator regulatory interactions, remains ambiguo…

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Reposted by Daniel Jost - Physical Biology of Chromatin group

Ranjith Padinhateeri @ranjithpa.bsky.social · 20/02/2026

Happy to share our Current Opinion review on the key challenges in accurately predicting 3D distances between chromatin segments and computing their dynamics. Thanks to @djost-physbiol.bsky.social @lucagiorgetti.bsky.social for the invitation to write this review! @shuvadipdutta.bsky.social

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 11/02/2026

🚨SUMOylation regulates the formation and function of Polycomb foci. New preprint from Cavalli lab where we performed modeling: SUMOylation regulates the PRC1 self-attractions mediating PcG condensate. Look at the preprint 👇https://www.biorxiv.org/content/10.64898/2026.02.05.704038v1

biorxiv.org

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 26/01/2026

Thanks Job ! Our modeling and conclusions nicely fits with your original findings. All comments are welcome as the paper is still far from being published :-)

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 26/01/2026

🤔 Open question: If sister chromatids are partially and asymmetrically aligned, how does the cell ensure accurate repair and segregation?

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 26/01/2026

Our results are very similar to recent findings in mammals from @golobor.bsky.social and Gerlich labs, suggesting possible conserved properties.

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 26/01/2026

We show that cohesin is sparsely distributed, leading to loosely aligned, mildly compacted sister chromatids. Moreover, cohesion is asymmetric—sister chromatids are tethered more strongly in cohesin-rich regions, even if they’re non-homologous.

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 26/01/2026

Our study combines: ✔️ Polymer modeling ✔️ Data analysis of Genome-wide contact maps (WT vs. mutants) of SisterC, Hi-C and Micro-C datasets.

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 26/01/2026

How does cohesin keep sister chromatids together and organize them after DNA replication? Cohesin isn’t just a “glue” for sister chromatids (cohesion function)—it also shapes their 3D organization via loop extrusion. But how do these two functions interact?

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 26/01/2026

🚨 New preprint from the lab. Combining modeling and data analysis of SisterC data in yeast, we investigate cohesion of replicated chromosomes and show that sister chromatids are loosely and asymmetrically aligned in G2/M. Check the tweetorial below ⬇️ www.biorxiv.org/content/10.6...

biorxiv.org

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Reposted by Daniel Jost - Physical Biology of Chromatin group

Aurele Piazza @aurelepiazza.bsky.social · 06/01/2026

SMC and recombination enthusiasts: we updated our work describing the loop extrusion properties of budding yeast condensin and its function in biasing donor usage for mating-type switching. Lots of cool new data, check it out! www.biorxiv.org/content/10.1...

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Reposted by Daniel Jost - Physical Biology of Chromatin group

Lennart Hilbert @lennarthilbert.bsky.social · 15/01/2026

Our Mini Review went online last night in Current Opinion in Genetics & Development "Block copolymer concepts of how transcription organizes the stem cell genome" doi.org/10.1016/j.gd... Fig. 1: Stem cell–typical organization of the genome and transcription, and the block copolymer sorting concept.

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Reposted by Daniel Jost - Physical Biology of Chromatin group

Oded Rechavi @odedrechavi.bsky.social · 15/10/2025

BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/09/2025

Chromatin gets stiffer when pulled gently but softer when yanked hard—thanks to loop extrusion. SMCs may play the role fo shock absorbers, protecting the genome from weak mechanical perturbations but allowing adaptation for persistent strong stresses.

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Reposted by Daniel Jost - Physical Biology of Chromatin group

Laboratory of Biology and Modeling of the Cell @lbmcinlyon.bsky.social · 03/07/2025

"A la découverte des chromosomes", la conférence immersive imaginée par @djost-physbiol.bsky.social est en ligne sur la chaine Youtube du CNRS. www.youtube.com/watch?v=CHh_... @cnrsbiologie.bsky.social @cnrs-rhoneauvergne.bsky.social @ensdelyon.bsky.social

youtube.com

À la découverte des chromosomes | Conférence immersive | Les Échappées inattendues

YouTube video by CNRS

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

8/ 💡 Big picture: Replication doesn’t just use the genome’s 3D structure—it actively reshapes it. Our model bridges replication dynamics with genome architecture, offering a new view of chromatin duplication in 3D.

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

7/ Our model also reveals a dynamic effect: As forks move, they temporarily slow down chromatin motion. Why? Due to the mechanical constraints of fork passage and intertwining of sister chromatids. 🧷

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

6/ Zooming out: replication forks are not evenly spread in early S-phase. They concentrate at one nuclear pole, then redistribute more evenly later on. This spatial bias could explain how forks cluster into Replication Foci, seen in microscopy! 🧪

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

5/ Then we asked: does this pattern exist in real cells? ✅ We confirmed it in vivo using new Hi-C data collected during early S-phase thanks to a collaboration with @aurelepiazza.bsky.social And it holds across different conditions. Importantly, it’s: 🔄 Replication-dependent 🚫 Cohesin-independent

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

4/ What did we find? A striking “fountain” pattern forms around early origins of replication, caused by the colocalization of sister forks moving outward. This pattern emerges spontaneously from our model! 🚰

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

3/ We built a computational model of the yeast genome that integrates: 📍Realistic 3D chromatin architecture 🕒 Accurate replication timing With this, we simulated how replication unfolds spatially inside the nucleus.

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

2/ DNA replication doesn’t happen in isolation—it’s tightly linked to how chromatin is organized in space. But from the behavior of sister replication forks to the formation of replication domains, many mechanisms are still debated. So, we turned to modeling. 💻

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 29/04/2025

🧵1/ How does DNA replication shape the 3D structure of the genome? 🧬 Despite major advances, we still don’t fully understand how replication and chromosome architecture interact. In our new study, we dive into this interplay using Saccharomyces cerevisiae as a model. 🔬

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Daniel Jost - Physical Biology of Chromatin group @djost-physbiol.bsky.social · 24/01/2025

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