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Reposted by Bruno Di Stefano

Genes & Development @genesdev.bsky.social · 22/06/2026

🆕 ADVANCE ONLINE 🆕 REVIEW: Mechanisms coordinating exit from the stem cell state in mammals By Park, Levin-Ferreyra and Bruno Di Stefano ➡️ ow.ly/siz250Za9Wh Bruno Di Stefano Baylor College of Medicine #stemcell #embryogenesis #celldifferentiation

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Reposted by Bruno Di Stefano

brumbaugh-lab.bsky.social @brumbaugh-lab.bsky.social · 09/06/2026

Check out our new preprint: (www.biorxiv.org/content/10.6...) that highlights estrogen signaling in trophoblast development. Our data suggest that increased dosage of NRIP1 impairs this pathway, contributing to placental defects linked to Down syndrome. Great work @deirdrelogsdon.bsky.social & team!

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Bruno Di Stefano @distefanolab.bsky.social · 13/05/2026

My lab @bcmhouston.bsky.social is hiring a postdoctoral fellow to study hematopoiesis and leukemia using innovative degron mouse models, RNA recording technologies, and functional genomics approaches. If you are interested, please see the position details below:

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Reposted by Bruno Di Stefano

Stem Cell Reports @stemcellreports.bsky.social · 06/12/2025

Dr. Bruno Di Stefano (Bruno Di Stefano) Stem Cell Reports Early Career Editor , will be at the American Society of Hematology Annual Meeting in Florida, USA 6-9 December 2025. Connect with him to discuss whether your research is fit for publishing in Stem Cell Reports!

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Reposted by Bruno Di Stefano

ISSCR @isscr.org · 25/11/2025

Elevate your career in stem cell science! Become an ISSCR member to enjoy exclusive benefits: complimentary webinars, preferred rates for events including ISSCR 2026, publishing benefits, and access to an international network. Join today 👉 bit.ly/4eOL2H1 Bruno Di Stefano

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Reposted by Bruno Di Stefano

Stem Cell Reports @stemcellreports.bsky.social · 21/11/2025

Meet Early Career Editor Bruno Di Stefano (Bruno Di Stefano) at #ASH2025 in Orlando, USA 6-9 December 2025. Connect with Dr. Di Stefano to discuss whether your research is a fit for publishing in Stem Cell Reports!

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

8/ Huge thanks to our editor and reviewers for invaluable feedback, and to our funders NIH, @ash.hematology.org , @worldwidecancer.bsky.social, @bepositivefdn.bsky.social, Webb-Waring Biomedical Research Awards, and CPRIT for supporting this work!

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

7/ Our findings establish a fundamental, conserved role for P-bodies in cell fate specification and reveal novel strategies to manipulate RNA condensates for directing cell identity in regenerative medicine.

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

6/ Applying these insights to stem cell differentiation, we show that manipulating P-body assembly or specific microRNA activity can direct pluripotent stem cells toward totipotency or the germ-cell lineage.

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

5/ What drives the selective sequestration of mRNAs in P-bodies? We found that certain cell-type–specific microRNAs are enriched in P-bodies and modulate RNA sequestration. Disrupting microRNA–target interactions prevents the corresponding mRNAs from being sequestered.

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

4/ Dissolving P-bodies in mouse naïve ESCs increased ribosome occupancy of P-body–enriched RNAs, including 2C transcripts, triggering reversion to a 2C-like state. This shows that releasing RNAs from P-bodies enables their translation and drives cell fate transitions.

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

3/ Surprisingly, P-body contents don't simply reflect current gene expression but are enriched with transcripts from the prior developmental stage. For example, in human naïve pluripotent ESCs, P-bodies sequester RNAs linked to the totipotent 8-cell embryonic stage.

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

2/ Using fluorescence-activated particle sorting, we profiled P-body contents across developmental stages and vertebrate species. P-body composition is cell-type specific, and the sequestered transcripts are translationally repressed.

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Bruno Di Stefano @distefanolab.bsky.social · 28/10/2025

1/ Excited to share our new study with @brumbaugh-lab.bsky.social, out in @natbiotech.nature.com! P-bodies selectively sequester RNAs encoding cell fate regulators, often from the preceding developmental stage. Releasing these RNAs can drive changes in cell identity. 🧵 www.nature.com/articles/s41...

nature.com

Selective RNA sequestration in biomolecular condensates directs cell fate transitions - Nature Biotechnology

Stem cell differentiation is controlled by manipulating RNA condensates.

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Bruno Di Stefano @distefanolab.bsky.social · 25/09/2025

Excited to present at the CSHL Cell Fate Conversions 2025 meeting! Connect with me there to discuss whether your research is a fit for publishing in @stemcellreports.bsky.social. invt.io/1bxba296yhc #cshldirect

invt.io

Check out ISSCR's society journal, Stem Cell Reports!

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Bruno Di Stefano @distefanolab.bsky.social · 03/07/2025

Excited to join the Stem Cell Reports team as an Early Career Editor! Grateful for the opportunity to contribute to the journal alongside fantastic colleagues. I encourage you to submit your work for consideration! @isscr.org @stemcellreports.bsky.social

invt.io

Check out ISSCR's society journal, Stem Cell Reports!

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Bruno Di Stefano @distefanolab.bsky.social · 12/05/2025

New preprint from the lab! With @brumbaugh-lab.bsky.social , we show that P-bodies sequester developmentally relevant RNAs to influence cell fate. miRNAs direct this conserved regulatory process, and manipulating it enhances totipotency and germ cell programming. www.biorxiv.org/content/10.1...

biorxiv.org

Selective RNA sequestration in biomolecular condensates directs cell fate transitions

Recent studies have emphasized the significance of biomolecular condensates in modulating gene expression through RNA processing and translational control. However, the functional roles of RNA condens...

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Reposted by Bruno Di Stefano

Peggy Goodell @goodell-lab.bsky.social · 06/03/2025

Thrilled to share our new work on #aging in the murine hematopoietic system! There is a lot here- stem cell numbers, self-renewal rates, mutation rates, mutation signatures, clonal fitness, environmental effects...

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Bruno Di Stefano @distefanolab.bsky.social · 28/01/2025

5/ Recent technological advancements have powered these studies, enabling answers to previously unanswerable questions (Fig. 4). Despite this progress, critical evaluation of the evidence concerning condensate properties is essential to understand their role in immune cell fate.

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Bruno Di Stefano @distefanolab.bsky.social · 28/01/2025

4/ Conversely, dysregulation of condensates has been linked to deleterious immune cell fates, including impaired function, autoimmunity, aging, and malignant transformation (Fig. 3).

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Bruno Di Stefano @distefanolab.bsky.social · 28/01/2025

3/ Recent studies have implicated condensates in the transcriptional and post-transcriptional processes that drive proper immune cell fates, from the level of hematopoietic stem cells to innate and adaptive immune cells (Fig. 2).

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Bruno Di Stefano @distefanolab.bsky.social · 28/01/2025

2/ Condensates are diverse, membraneless assemblies of nucleic acids and proteins (Fig. 1). They are thought to enable temporally precise control of gene expression by concentrating or segregating components of the gene regulatory machinery and their targets.

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Bruno Di Stefano @distefanolab.bsky.social · 28/01/2025

1/ Pleased to share our review, published today @natrevimmunol.bsky.social, highlighting how biomolecular condensates enhance the precision and flexibility of gene regulatory networks that guide fate decisions during normal and pathological immune cell development. www.nature.com/articles/s41...

nature.com

Biomolecular condensates in immune cell fate - Nature Reviews Immunology

Recent studies suggest that biomolecular condensates — membrane-less assemblies of proteins and nucleic acids — are involved in regulating gene expression to ensure proper immune cell development. Thi...

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Bruno Di Stefano @distefanolab.bsky.social · 13/12/2024

Great thread from @levin-ferreyra.bsky.social about her latest work in the lab, out today in @emboreports.bsky.social ! 👇

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