iiinova — where your digital self keeps evolving

Reposted by Andy DeLaitsch

Przemek Dutka @przemekdut.bsky.social · 08/10/2026

Our latest development is now on bioRxiv: cryoSHIELDs — amphipathic peptide additives that make cryo-EM grid prep far more reliable. Tested across 24 protein systems, they enabled the first ABCC6 structure and GPCR–G protein structures at just 0.1–0.5 mg/mL! www.biorxiv.org/content/10.6...

14726

Andy DeLaitsch @delaitsch.bsky.social · 20/08/2026

Fourier transform is top of that list for me. I think my understanding and execution of structural biology projects improved dramatically late-PhD when I had a better grasp of thinking in terms of Fourier space.

130

Reposted by Andy DeLaitsch

Alisia Fadini @alisiafadini.bsky.social · 01/04/2026

ROCKET 🚀 inference-time optimization of AlphaFold to fit structural data is published! rdcu.be/fa9YH Since our preprint, we’ve pushed it to regimes where other methods break: low resolution, weak signal, real experimental edge cases. Here’s what we learned: 1/15

rdcu.be

AlphaFold as a prior: experimental structure determination conditioned on a pretrained neural network

Nature Methods - ROCKET improves experimental structure elucidation by integrating implicit structural knowledge from OpenFold, a trainable reimplementation of AlphaFold2, with X-ray...

112751

Andy DeLaitsch @delaitsch.bsky.social · 21/01/2026

Was such a great class last year! I’d highly recommend to ppl getting into tomography

010

Reposted by Andy DeLaitsch

cryoEM papers @cryoempapers.bsky.social · 05/11/2025

Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells pubmed.ncbi.nlm.nih.gov/41182991/ #cryoEM

054

Andy DeLaitsch @delaitsch.bsky.social · 06/10/2025

There's a new best-in-class HIV-1 antibody out today! Happy to have contributed to the structural characterization of this, and two other distinct VRC01-class bNAbs isolated from a single individual. www.nature.com/articles/s41...

a, Schematics illustrating the position and length of FWRH1 and/or FWRH3 insertions of 04_A06 (left), 01_D03 (middle) and 05_B08 (right; top), EM maps showing side views of 04_A06, 01_D03 and 05_B08 bnAb Fabs in complex with BG505SOSIP.664 Env trimers (middle) and insets illustrating a close-up of bnAb interactions with the adjacent gp120 protomer (gp1202; bottom); HC, heavy chain; LC, light chain. b, Canonical interactions of CD4 and bnAbs to the CD4bs (04_A06, IOMA and VRC01) with Env gp120 D368, the F43 pocket and gp120 N280/R456. c, Interactions between the CDRH1s of 04_A06 (blue cartoon representation) and 1-18 (red cartoon representation) with the secondary (gp1202) Env protomer, shown as a surface colored by percent conservation. The inset (top right) shows the Env trimer–04_A06 complex as a surface representation for orientation. d, Crystal structure of unbound 04_A06 Fab (bottom) and inset highlighting the 04_A06 CDRH1, with electron density contoured at 1.5σ (top). e, Molecular surface representation showing the surface area buried by VRC01-class bnAbs (04_A06, 01_D03, 05_08, 3BNC117 and VRC01) on primary (gp1201) or secondary (gp1202) Env protomers (top). Listing of Protein Data Bank (PDB) accession codes, breadth (%), heavy chain insertion length and position, buried surface area (BSA) on gp1201 and gp1202 as well as total BSA for VRC01-class bnAbs (bottom).

110

Andy DeLaitsch @delaitsch.bsky.social · 01/10/2025

From what I gather, CXCR4 (previously called fusin) was identified as a co-receptor for HIV nearly 30 years ago (pubmed.ncbi.nlm.nih.gov/8629022/). Nice to see a structure, and interesting to see it was done with HIV-2 gp120.

pubmed.ncbi.nlm.nih.gov

000

Reposted by Andy DeLaitsch

Caitie McCafferty @computingcaitie.bsky.social · 23/09/2025

I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪

716454

Reposted by Andy DeLaitsch

Debnath Ghosal @debnathghosal.bsky.social · 02/08/2025

📣 New paper alert! Just out in Cell Reports! pubmed.ncbi.nlm.nih.gov/40644298/ Thrilled to share that we have discovered a brand-new anti-phage defense system! Bacteria have evolved various defense strategies (CRISPR etc) to counter phage attacks. We found a new one - fascinating and dramatic ⚔️🦠❄️🔬

315153

Reposted by Andy DeLaitsch

Alister Burt @alisterburt.bsky.social · 24/07/2025

If Management is the Only Way Up, We're All F'd - Rand Fishkin sparktoro.com/blog/if-mana...

011

Reposted by Andy DeLaitsch

Daniel Hurdiss @danielhurdiss.bsky.social · 18/06/2025

Latest preprint from the lab, many years in the making! By combining #cryoEM with #AlphaFold3 modelling, we propose that norovirus NS3 forms a transmembrane RNA translocase. This could have big implications for our understanding of viral replication & assembly (🧵) www.biorxiv.org/content/10.1...

918354

Reposted by Andy DeLaitsch

Jacob Parres-Gold @jacobparresau.bsky.social · 26/02/2025

My first project in the @elowitzlab.bsky.social is finally out in @cellpress.bsky.social! We explore how competitive, "many-to-many" dimerization allows complex, multi-input, and cell-type-specific biochemical computations🧵↓ doi.org/10.1016/j.ce...

doi.org

Redirecting

1175

Reposted by Andy DeLaitsch

bioRxiv Biophysics @biorxiv-biophys.bsky.social · 14/01/2025

MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony www.biorxiv.org/content/10.1101/202…

0155

Reposted by Andy DeLaitsch

Derek Lowe @dereklowe.bsky.social · 10/12/2024

RSV vaccination attempts in young children have a troubled history. Which seems to be continuing:

science.org

Infant RSV Vaccination Trouble

1319

Andy DeLaitsch @delaitsch.bsky.social · 10/12/2024

100 years ago, De Broglie published his thesis, "On the Theory of Quanta". The ideas in it catalyzed the development of electron microscopy, though Ernst Ruska was apparently unaware of De Broglie's "material waves" when he and Max Knoll first started toying with the electron microscope.

100

Reposted by Andy DeLaitsch

Micha Rapé Lab @micharapelab.bsky.social · 03/12/2024

Very excited to present the structural and mechanistic basis for stress response silencing by the monster E3 ligase SIFI. www.biorxiv.org/content/10.1...

811635