Sign in

Deepa Rajan, PhD

@deeparajan.bsky.social
256 followers 71 following 55 posts

MD/PhD candidate at UCSF researching the intelligence of single cells research summary: www.youtube.com/watch?v=Noq8wtqZKWY

PostsRepliesMedia
Deepa Rajan, PhD @deeparajan.bsky.social · 23/07/2026
Spotify: open.spotify.com/podcast-chap... Apple: podcasts.apple.com/us/podcast/n...
open.spotify.com
Deepa Rajan: Crop Rotation and Childlike Exploration
Night Science · Chapter · 7m
010
Deepa Rajan, PhD @deeparajan.bsky.social · 23/07/2026
I'm excited to be featured on one of my favorite podcasts!! I binged @nightsciencepod.bsky.social during my PhD. Listen to this episode to hear about how the crop rotation system can be applied to the scientific creative process. And learn about our beloved Stentor in Every School program!
182
Deepa Rajan, PhD @deeparajan.bsky.social · 02/05/2026
Thanks so much for your mentorship in the early stages of this project!
010
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
Thank you so much, Flora!! Some of the CaMKII results are thanks to the early advice you gave me about ordering drugs from MedChemExpress :)
010
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
Sorry I just realized that bluesky inverted the coloring of the graphs after I uploaded them! You'll have to read the paper to see the real graphs :)
050
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
Link to paper in @CurrentBiology. Thank you to co-authors @aralbright.bsky.social @gautamdey.bsky.social and more. @ucsfhealth.bsky.social @ucsfmedicine.bsky.social @uofcalifornia.bsky.social www.cell.com/current-biol...
cell.com
Molecular pathways for learning in the single-cell Stentor coeruleus
Rajan et al. explore the molecular basis of habituation in the unicellular organism Stentor coeruleus using a combination of pharmacological methods, proteomics, transcriptomics, and RNAi. These exper...
7406
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
There is still much left to learn – for both Stentors and the humans who study them! Which other molecules drive Stentor habituation? What else can they learn? By researching how a single cell learns, we can begin to shed light on the origins of intelligence. 13/n
1241
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
Indeed, the habituated state can be propagated to daughter cells during cell division! 12/n
2150
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
But is this habituation memory localized? Can it persist in progeny? To test this, we trained cells with mechanical taps as they were dividing. The video below is sped up over 7 hours of training/dividing. 11/n
1190
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
These findings are consistent with a receptor-inactivation mechanism for Stentor habituation: mechanoreceptor senses stimulus ➡️ channel opening changes membrane potential ➡️ CaMKII activation ➡️ protein phosphatase activation ➡️ dephosphorylation of mechanoreceptors. 10/n
1180
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
We also knocked down SteCoe_6763, another calcium-binding RNA-seq candidate, using RNA interference and found that knocking down this protein accelerates habituation. We’re thinking of calling this protein “ignoramin” because getting rid of it improves learning! 9/n
1200
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
Calcium/calmodulin-dependent kinase II (CaMKII), one of the RNA-seq candidates, is essential for learning in animals. We found that KN-93, a drug that inhibits CaMKII, impairs habituation in Stentor cells. 8/n
1150
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
This suggests that forgetting is an active process that requires new protein synthesis. But which molecules are being synthesized/degraded? We did a series of RNA-seq and proteomics experiments to obtain molecular snapshots of cells during learning and forgetting. 7/n
1171
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
For decades, the prevailing story has been that we (multicellular animals) need to make new proteins to learn. Surprisingly, habituation in Stentor cells improves after treatment with drugs like puromycin that impair protein synthesis! 6/n
1170
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
When Stentors receive repeated weak mechanical stimulation, however, they habituate and thus stop contracting and remain in their elongated state. Below is a video of Stentors that have become habituated after an hour of repeated stimulation. 5/n
1191
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
When Stentors experience a mechanical stimulus, they contract into a ball-like shape – presumably to escape from predators lurking in the pond. 4/n
1180
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
Habituation is the gradual decrease in response after repeated stimulation. Humans do this, too! For example, we learn to ignore the feeling of clothes on our skin after wearing them all day. However, we have 86 billion neurons to help us learn while Stentor is a single cell! 3/n
1160
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
If you scoop up some water from a local pond and stick it under a microscope, you might just encounter Stentor coeruleus, a unicellular organism that is capable of a type of learning called habituation. 2/n
1170
Deepa Rajan, PhD @deeparajan.bsky.social · 23/04/2026
How can a single cell learn without a brain? We explore this in my paper with @wallaceucsf.bsky.social! We discovered that single cells may learn using molecules similar to those that animal brains use to learn, like CaMKII. Cells can also propagate memory states to their progeny!
426789
Deepa Rajan, PhD @deeparajan.bsky.social · 07/04/2026
www.nature.com/articles/s41...
nature.com
Stentor stipatus is a new unicellular species that demonstrates habituation and unique phototaxis - Scientific Reports
Stentor, the genus of large trumpet-shaped ciliates, is well-known for its complex morphology and striking behaviors. Members of this genus are distributed throughout the world in a wide and diverse p...
020
Deepa Rajan, PhD @deeparajan.bsky.social · 07/04/2026
We discovered a new species! Our paper on Stentor stipatus is now published @wallaceucsf.bsky.social @aralbright.bsky.social
1155
Reposted by Deepa Rajan, PhD
The B'K Magazine @thebkmagazine.bsky.social · 29/03/2026
Just a few more days until we launch The B'K Volume 17, Issue 2 featuring poetry by: Ashen Speir, Cypher, David Anson Lee, @deeparajan.bsky.social, Jamez Terry, Joni Thomas, Kieran Fu, Leslie Dianne, Maxochitl Cortez, Muheez Olawale, and Theodora Bonis. Stay tuned!
A drawing in sepia tones of a child looking at the reader. In the upper left corner are the logos for The B'K and Global Majority Press. Text says Volume 17, Issue 2, and has the list of creatives. Text says Launching April 1.
012
Deepa Rajan, PhD @deeparajan.bsky.social · 21/02/2026
link to tickets! www.eventbrite.com/e/lectures-o...
eventbrite.com
Lectures on Tap - Learning Without a Brain: How Intelligence Begins Small
Professors, experts, and storytellers giving thought-provoking lectures in SF bars.
000
Deepa Rajan, PhD @deeparajan.bsky.social · 21/02/2026
If you're in SF, come celebrate St. Patrick's day with me and learn about how single cells learn! I'm giving a lecture about my research at a bar as part of the Lectures on Tap series🍻
100
Deepa Rajan, PhD @deeparajan.bsky.social · 23/01/2026
dissertation book haul!
010
Reposted by Deepa Rajan, PhD
Phil Lewis @phillewis.bsky.social · 08/09/2025
Trump minimizes domestic abuse during a talk at the Museum of the Bible: "Things that take place in the home, they call crime ... If a man has a little fight with the wife, they say this was a crime, see?"
52734641256
Reposted by Deepa Rajan, PhD
Orit Peleg @oritpeleg.bsky.social · 25/06/2025
Help us record firefly flashes! 👇🙏
56654
Reposted by Deepa Rajan, PhD
Ambika Nadkarni @biochembika.bsky.social · 25/06/2025
Our research on wound resistance in Stentor is live on bioRxiv! Tldr; we squeezed the cells through microfluidic constrictions and the formidable lead author Rajorshi Paul characterized what makes the cells get wounded!
112
Deepa Rajan, PhD @deeparajan.bsky.social · 25/06/2025
thank you!!
110
Deepa Rajan, PhD @deeparajan.bsky.social · 24/06/2025
Our paper on single-cell learning is now published in @currentbiology.bsky.social! @wallaceucsf.bsky.social sciencedirect.com/science/arti...
sciencedirect.com
A receptor-inactivation model for single-celled habituation in Stentor coeruleus
The single-celled ciliate Stentor coeruleus demonstrates habituation to mechanical stimuli, but the mechanism of learning in this single cell, which l…
13615
Deepa Rajan, PhD @deeparajan.bsky.social · 12/04/2025
Stentor in Every School: t.co/2AalQ8y91l
t.co
https://centerforcellularconstruction.org/stentor-in-every-school/
030
Deepa Rajan, PhD @deeparajan.bsky.social · 12/04/2025
I'm leading a program at the Exploratorium @exploratorium.bsky.social this weekend! Come check out "Mini Microbe Missions", an opportunity for kids (and adults) to do citizen science using the single cell Stentor coeruleus, supported by our Stentor in Every School initiative t.co/VX8r9BHxsJ
2101
Reposted by Deepa Rajan, PhD
Arjun Raj @arjunraj.bsky.social · 05/04/2025
Went to a wonderful reunion for the birthday of dear friend and colleague Gerd Blobel yesterday. In the end, it’s funny how all the papers and grants fade away and the main thing you remember are the people.
2588
Reposted by Deepa Rajan, PhD
jamelle @jamellebouie.net · 16/03/2025
pete hegseth carrying out his effort to resegregate the military www.military.com/daily-news/2...
military.com
Marines with Skin Condition Affecting Mostly Black Men Could Now Be Booted Under New Policy
A new Marine Corps policy says troops with a genetic skin condition that can cause pain and scarring from shaving and mainly affects Black men can be separated if the health issue persists.
495117253727
Deepa Rajan, PhD @deeparajan.bsky.social · 08/03/2025
channeled my inner Joan Baez at the Stand Up for Science protest today
060
Reposted by Deepa Rajan, PhD
Ben Allwein @benallwein.bsky.social · 06/03/2025
I am so proud to share a major part of my graduate school research, published today in Science, where we use cell-free replication assays and cryo-EM to describe a central mechanism of eukaryotic DNA replication: www.science.org/doi/10.1126/...
science.org
G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation
DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...
0175
Reposted by Deepa Rajan, PhD
Itai Yanai @itaiyanai.bsky.social · 03/03/2025
When your kids ask you what you did to save US science in the crisis of ‘25, you will say that you showed up! Please spread the word and join us!! @standupforscience.bsky.social
217675
Reposted by Deepa Rajan, PhD
Mark A. Hanson @hansonmark.bsky.social · 08/02/2025
Today they went nuclear. Imagine all firefighters were told "You all still have salaries, but as of today the government will no longer pay for building maintenance, firetruck maintenance and repair, hydrant upkeep, or admin assistants." That's what they're doing to US universities.
315562
Reposted by Deepa Rajan, PhD
Wallace Marshall @wallaceucsf.bsky.social · 24/01/2025
transcription people: are there examples where a transcriptional program gets re-set by cell division?
4115
Reposted by Deepa Rajan, PhD
Jerry Chen @jcsalterego.bsky.social · 19/01/2025
you know what *is* available. TiK ToK by kesha
875284759
Reposted by Deepa Rajan, PhD
yoonji kim @yoonjikim.bsky.social · 14/01/2025
HNY! In case you thought 2025 is the year of the snake, it's actually the year of the SQUID 🦑 Excited to present this new protocol paper from the @brangwynnelab.bsky.social on isolating+transfecting squid primary cells 🧫✨! Check it out here: www.biorxiv.org/content/10.1...
biorxiv.org
Squid primary cell culture as a model system and experimental tool
Squid primary cells from various tissues and ages are isolated, maintained in culture, and express exogenous genes. This protocol opens up numerous opportunities in molecular biology, neuroscience, an...
1167
Deepa Rajan, PhD @deeparajan.bsky.social · 13/01/2025
yes was so great to meet you in person!!
010
Deepa Rajan, PhD @deeparajan.bsky.social · 13/01/2025
I brought live Stentor cells with me on the train and have regaled a few fellow passengers with tales of this mysterious giant cell that can learn #scicomm
030
Deepa Rajan, PhD @deeparajan.bsky.social · 13/01/2025
after a week in Chicago for the Biological Systems that Learn workshop @nitmb.bsky.social, I've been traveling back to SF via train over ~55 hours. Today is Day 3 on Amtrak for me and I've never experienced more scenic backdrops for working on a manuscript
2120
Reposted by Deepa Rajan, PhD
zara weinberg @weinberz.witchlab.org · 07/01/2025
love learning about the neurobiology of the two genders (lordosis and aggression) 🫠
052
Reposted by Deepa Rajan, PhD
Wallace Marshall @wallaceucsf.bsky.social · 23/12/2024
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology www.biorxiv.org/content/10.1...
626892
Reposted by Deepa Rajan, PhD
Wallace Marshall @wallaceucsf.bsky.social · 21/12/2024
Delighted to share our new preprint on the cell biology and genome of Stentor pyriformis, a giant ciliate that maintains Chlorella endosymbionts in surface-associated microtubule baskets. www.biorxiv.org/content/10.1...
1228375
Deepa Rajan, PhD @deeparajan.bsky.social · 21/12/2024
if it's behind a paywall for you, here's the pdf: online.fliphtml5.com/UCSFcellbio/...
online.fliphtml5.com
2024 Current Biology dispatch article
023