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Debnath Ghosal

@debnathghosal.bsky.social
455 followers 453 following 119 posts

CryoEM, Structural Cell Biology. Group Leader@University of Melbourne. Formerly at @Caltech, @MRC_LMB, Cambridge University.

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Reposted by Debnath Ghosal
Éric Hanssen @bigfrenchman.bsky.social · 06/10/2026
It has been quite a while but here is our application for square apertures in cell and structural biology www.biorxiv.org/content/10.6...
biorxiv.org
Whole cryo-FIB lamella acquisition at high magnification with rectangular beam montage cryo-electron tomography
The visualisation of large biological samples in cryo-electron tomography requires both few-nanometre resolution and a wide, ideally >10 µm, field of view. For a single image, increasing magnification...
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Debnath Ghosal @debnathghosal.bsky.social · 07/10/2026
When imaging at high mag using cryo-ET, field of view reduces. How to overcome this limit? How do we get a "bird’s-eye view" without compromising resolution? Our very own @HamishBrown has a solution to this: “montage tomography with a rectangular beam!” www.biorxiv.org/content/10.6...
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
Heroic effort from #AnanyaPadmakumar! Also, amazing collaboration involving #EricReynolds, #PaulVeith, #PaulMcMillan, #StuartDashper! Thanks to all the support from @unimelb.edu.au, @ccemmp-outreach.bsky.social, @unimelbMDS, @CGCPT, @bio21.bsky.social. .
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
The big idea: We show disease-associated microbiomes form reproducible, distinct biofilms that may provide a new axis for predicting, diagnosing & targeting microbial disease. So in microbiome, - it is not just about WHO is there! - it is about WHERE they are & HOW they organise!
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
🧩 We followed a subset of patients. Longitudinal study showed that after therapy, the “test-tube brush” biofilms disappeared, while “corncob” biofilms emerged, alongside resolution of disease. So, biofilm architectures and compositions changed with the disease state. 🔄
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
Now the really interesting part! The "test-tube brush" biofilms were markedly enriched in severe periodontal disease, and their burden increased with disease severity/pocket depth. Notably, their presence was dramatically increased in smoking-associated disease sites.
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
We resolved the architecture and taxonomic composition of these biofilms using specific FISH probes. Most of these biofilm architectures were not described previously, and they form in OUR mouth during oral microbiome dysbiosis!!
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
What we found was remarkable 🤯🦠 These bacteria don't simply pile up. They assemble into reproducible multicellular architectures, resembling: 🌽 Corncobs, 🧪 Test-tube brushes, 🦔 Echidnas, 🌿 Achillea, 🌊 Flowing streams, etc.
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
Are they a chaotic mass of mixed bacteria? We went looking. 🔬🦠 We collected dense polymicrobial biofilms from periodontal pockets across 174 sites from 36 patients. Using multiplex FISH/CLSM/spectral imaging, we mapped the architectures & compositions of these complex biofilms!
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
Our mouth harbours 700+ bacterial species & billions of them. When the oral microbiome becomes dysbiotic, microbes can go rogue & form dense, pathogenic biofilms, causing periodontitis & contributing to many systemic diseases (oral cancer, diabetes, cognitive disorders etc).
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Debnath Ghosal @debnathghosal.bsky.social · 02/10/2026
New preprint alert! 🦠🔬 We show how pathogenic bacteria form diverse, previously unknown biofilms within a dysregulated oral microbiome. A fascinating glimpse into how microbial communities may contribute to disease! www.biorxiv.org/content/10.6...
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Debnath Ghosal @debnathghosal.bsky.social · 23/09/2026
Huge congratulations to @ShubhaUdupa on her brilliant thesis oration! 🎓 Shubha is a joint PhD student between my lab and Prof Marc Erhardt’s lab at Humboldt Universität. Shubha resolved a ~80-year-old, clinically highly relevant problem in bacterial cell biology. 🦠🔬. Onwards and upwards, Shubha!
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Debnath Ghosal @debnathghosal.bsky.social · 16/09/2026
Very pleased to see our recent work on the evolution of complex eukaryotic cells from simple microbial ancestors is featured in this @nature.com News article. www.nature.com/articles/d41....
nature.com
These bizarre, much-coveted microbes are revealing the origins of complex life
Researchers are racing to culture a menagerie of exotic organisms and finding lots of surprises along the way.
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Debnath Ghosal @debnathghosal.bsky.social · 15/08/2026
Also, yay! Now we have imaged viruses from all three domains of life!
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Debnath Ghosal @debnathghosal.bsky.social · 15/08/2026
Amazing to be a part of this fantastic work led by the @brendanburns999.bsky.social lab! Here, for the first time, we visualized unique viruses associated with Asgard archaea using cryoET. You can read it here: lnkd.in/gs7mztKw
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Reposted by Debnath Ghosal
Bronte Johnstone @bjohnstone.bsky.social · 02/08/2026
I am delighted and incredibly grateful to share that I have been awarded a NHMRC Emerging Leadership (EL1) Investigator Grant!
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Debnath Ghosal @debnathghosal.bsky.social · 06/07/2026
Had an amazing day at the @Sydney_Uni, presenting our work at the SABA BacChats Seminar Series! Great scientific discussions throughout the day. Thanks again for the warm hospitality, I thoroughly enjoyed the visit! @doylemt1.bsky.social and @BilgeErcan, thank you both for everything.
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Reposted by Debnath Ghosal
Alex Crits-Christoph @acritschristoph.bsky.social · 13/06/2026
This Visual-omics approach is kind of amazing: "We resolved each to sufficient resolution (2.8 and 3.6 Å) to predict the amino acid composition directly from the 3D electrostatic potential map"
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Reposted by Debnath Ghosal
Luca Troman @lucatroman.bsky.social · 13/06/2026
Excited to be presenting this work today at the 3DEM Gordon Research Seminar and later this week at the Gordon Research Conference. Looking forward to the discussions and feedback! www.grc.org/three-dimens...
grc.org
2026 Three Dimensional Electron Microscopy Conference GRC
The 2026 Gordon Research Conference on Three Dimensional Electron Microscopy will be held in Castelldefels, Barcelona Spain. Apply today to reserve your spot.
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Reposted by Debnath Ghosal
STCmicrobeblog @stcmicrobeblog.bsky.social · 13/06/2026
including state-of-the-art #CryoET #CryoEM #teamtomo
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Debnath Ghosal @debnathghosal.bsky.social · 14/06/2026
The CPR has a very high host specificity! We have not tested just the filament! These are all great experiments!
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
Terrific effort led by @lucatroman.bsky.social with support from #JackKim, @tiltedscientist.bsky.social, #JaysonRose. Amazing collaboration with @PetrovskiLab and @banfieldlab.bsky.social. @unimelb.edu.au, @FMDHS, @ccemmp-outreach.bsky.social, @bio21director.bsky.social
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
This study further highlights the power of integrating advanced cryo-EM with computational structural biology to assign functions to the vast repertoire of proteins encoded within microbial “dark matter,” where conventional annotation approaches often fall short.
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
Phylogenetic analysis revealed that the Pam system is widespread exclusively within CPR bacteria, with no homologs identified in non-CPR lineages, indicating that Pam pili are specific to and unique within this superphylum.
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
The pilins have Ig-like folds and assemble via donor-strand exchange. The filaments show some similarities to the Pap/Fim pilus systems, so we named them “Pam” filaments (remember the Melbourne “bird”?).
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
By using visual-omics, we managed to assign the molecular identity of these filaments directly from structure, without relying on sequence homology. It turned out to be a completely novel filament system, never before described in any bacteria.
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
What remained cryptic was the identity of the filaments that JR1 uses to lock onto their prey. Using cryo-EM, we solved the structure of the filament at high resolution, but most CPR proteins lack obvious sequence homologs and have very poor functional annotation.
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
CPR bacteria form a superphylum that may account for 15–50% of all bacterial diversity (!!), yet we know remarkably little about them. Recently, we showed that the dwarf CPR bacterium JR1 entangles its host with specialized filaments and kills using a lance-like structure. 🦠⚔️
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Debnath Ghosal @debnathghosal.bsky.social · 12/06/2026
New paper alert!! We just discovered a new filament system that is widespread exclusively within CPR bacteria! www.biorxiv.org/content/10.6.... Short thread below. 🦠🔬🚀
biorxiv.org
A Novel Pilus System in Candidate Phyla Radiation Bacteria
The Candidate Phyla Radiation (CPR) represents a bacterial superphylum estimated to include between 15–50% of all bacterial species, yet CPR bacteria remain challenging to culture and have been primar...
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Debnath Ghosal @debnathghosal.bsky.social · 10/06/2026
Amazing news @gautamdey.bsky.social. Congratulations!!
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Reposted by Debnath Ghosal
Harris Wang @harriswang.bsky.social · 01/05/2026
1/ Excited to share our new paper in Science @science.org: “Toward life with a 19-amino acid alphabet through generative artificial intelligence design.” A great collab w/ Sergey's group @sokrypton.org at MIT @columbiasysbio.bsky.social science.org/doi/10.1126/... 🦠🧬🛠️🖥️💥
science.org
Toward life with a 19–amino acid alphabet through generative artificial intelligence design
Because all known living organisms are made from at least 20 canonical amino acids, the feasibility of life using a more simplified alphabet remains unclear. In this work, we leveraged computational d...
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Debnath Ghosal @debnathghosal.bsky.social · 15/04/2026
This was amazing team effort!
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Debnath Ghosal @debnathghosal.bsky.social · 14/04/2026
Many thanks Craig
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Debnath Ghosal @debnathghosal.bsky.social · 14/04/2026
Indeed, this is very exciting
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Debnath Ghosal @debnathghosal.bsky.social · 14/04/2026
We would love to know this. We donot know how these microbes sense their environment and partners.
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Debnath Ghosal @debnathghosal.bsky.social · 12/04/2026
Great effort from @tiltedscientist.bsky.social @dcshepherd.bsky.social @bindusmitapaul.bsky.social. Thanks to @unimelb.edu.au @FMDHS @ccemmp-outreach.bsky.social @CGCPT @bio21director.bsky.social
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Debnath Ghosal @debnathghosal.bsky.social · 12/04/2026
Really excited about this work, you can read more here: www.cell.com/current-biol.... Wonderful collaboration with Brendan Burns, Iain Duggin and Katharine Michie labs.
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Debnath Ghosal @debnathghosal.bsky.social · 12/04/2026
From these cultures, after years of effort, we captured the first direct physical interaction between an Asgard archaeon and a bacterium via nanotubes, offering a rare glimpse into an event that may have led to the emergence of the first eukaryotic cell billions of years ago.
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Debnath Ghosal @debnathghosal.bsky.social · 12/04/2026
We analysed microbial mats from stromatolites in Shark Bay, Australia – environments that resemble early Earth and act like ~living fossils or time capsules of evolutionary history. These environments host diverse microbial communities akin to those that thrived in ancient times.🔬❄️🧬 🦠
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Debnath Ghosal @debnathghosal.bsky.social · 12/04/2026
One of biggest mysteries in biology: how did complex eukaryotic cells evolve from simple microbes? ~1.8 billion years ago, an archaeal cell likely merged with a bacterium to form the first eukaryotic cell, but can we ever find direct evidence of this transformative event? 🦠 🚶‍♂️
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Debnath Ghosal @debnathghosal.bsky.social · 14/03/2026
This is amazing work! Congratulations.
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Reposted by Debnath Ghosal
Tracy Palmer @proftracypalmer.bsky.social · 12/03/2026
2 year fellowship available in AMR research www.medicalresearchfoundation.org.uk/grants/antim...
medicalresearchfoundation.org.uk
Medical Research Foundation | Antimicrobial Resistance Research
Antimicrobial Resistance Research
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Reposted by Debnath Ghosal
Ben Long @botbml.bsky.social · 10/03/2026
Emma Johnston (1973–2025) | Nature Ecology & Evolution share.google/8C3YzLRFM9QC...
share.google
Emma Johnston (1973–2025) - Nature Ecology & Evolution
Innovative marine ecologist, passionate science communicator and visionary leader in higher education.
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Debnath Ghosal @debnathghosal.bsky.social · 13/02/2026
Thank you so much Kate
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Reposted by Debnath Ghosal
Kate Michie @kmichie.bsky.social · 12/02/2026
Beautiful work Deb! Check out these structures? Biofilms are very interesting. 🧶🧬
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Debnath Ghosal @debnathghosal.bsky.social · 12/02/2026
Great collaboration with #ZavialovLab, #UhlinLab and #KnightLab. And terrific effort from our PhD student @bindusmitapaul.bsky.social @UniMelb @CCeMMP, @UniMelbMDHS, @CGCPT. Of course, #HenriMalmi and #NataliaPakharukova are two superstars who led this project!
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Debnath Ghosal @debnathghosal.bsky.social · 12/02/2026
Cryo-EM structures of individual pilus, pilus pairs, and multi-pilus stacks revealed the structural framework of 3D biofilms, informing novel therapeutic strategies. 🦠🔬
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Debnath Ghosal @debnathghosal.bsky.social · 12/02/2026
We used electron cryo-tomography to reveal that an extensive network of thin bacterial filaments (Csu pilus/pili) forms a flat, stacked meshwork that mediates cell–cell connections and assembles drug-resistant biofilms.
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Debnath Ghosal @debnathghosal.bsky.social · 12/02/2026
A. baumannii is one of the most notorious multidrug-resistant pathogens, capable of forming drug-tolerant biofilms that make infections exceptionally difficult to treat. But how are these complex microbial fortresses built? Just published in Nat Comms. pubmed.ncbi.nlm.nih.gov/41654547/
pubmed.ncbi.nlm.nih.gov
Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly - PubMed
Many Gram-negative nosocomial pathogens rely on adhesive filaments, known as archaic chaperone-usher pili, to establish stress- and drug-resistant, multi-layered biofilms. Here, we uncover the mechanism by which these pili build three-dimensional (3D) biofilm architectures. In situ analyses of Acine …
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Debnath Ghosal @debnathghosal.bsky.social · 06/02/2026
Many thanks @arianebriegel.bsky.social :-)
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