Sign in

Wolfgang Hess

@cyanolab.bsky.social
509 followers 175 following 50 posts

Interested in comparative & functional microbial genomics, RNA biology, native CRISPR systems, cyanobacteria, plant evolution & regulation of photosynthesis

PostsRepliesMedia
Wolfgang Hess @cyanolab.bsky.social · 2h
Bakteriopolis, now in Freiburg. A cool, interactive exhibition about the tiny ones. Mo-Fri 10:00 to 16:00, other times can be arranged.
096
Wolfgang Hess @cyanolab.bsky.social · 07/10/2026
The Megataxonomy of viruses. comptes-rendus.academie-sciences.fr/biologies/ar...
comptes-rendus.academie-sciences.fr
Organization and evolution of the virosphere and the replicator space
010
Wolfgang Hess @cyanolab.bsky.social · 19/08/2026
Cyanobacterial RNA-binding protein Asl3888 is ribosome-associated and involved in C/N metabolism. It is encoded in almost all cyanobacteria, green, red, and brown algae, diatoms, cryptomonads, and pelagophytes: www.biorxiv.org/content/10.6... @mygalperin.bsky.social
biorxiv.org
0111
Wolfgang Hess @cyanolab.bsky.social · 23/07/2026
RNA rules: Guanidine fuels rapid resurrection of desert cyanobacteria, controlled by a guanidine-specific riboswitch: www.pnas.org/doi/10.1073/...
pnas.org
Guanidine fuels rapid resurrection of desert cyanobacteria | PNAS
In desert ecosystems, microbial activity is driven by brief hydration pulses but is severely limited by persistent nutrient scarcity. Cyanobacteria...
0163
Wolfgang Hess @cyanolab.bsky.social · 23/07/2026
New in Nature: The planktonic microbiome of the Great Barrier Reef, showing the composition of the microbiome along a transect of the Great Barrier Reef in the Pacific Ocean. Lots of cyanobacteria and cyanophage genomes, here: www.nature.com/articles/s41...
nature.com
The planktonic microbiome of the Great Barrier Reef - Nature
The Great Barrier Reef Microbial Genomes Database compiles prokaryotic, viral and eukaryotic genomes from seawater collected from the Great Barrier Reef, providing a rich resource for the study of mar...
021
Reposted by Wolfgang Hess
CIBSS – Centre for Integrative Biological Signalling Studies @cibss.bsky.social · 13/07/2026
📣 #JobAlert The Faculty of Biology of @uni-freiburg.de invites applications for a Professorship (W3) for Bioinformatics and Molecular Genetics. ❗ Application Deadline: 15 August, 2026 🔗 kurzlinks.de/roi8 #Biology #Bioinformatics #MolecularGenetics
Job alert text with logo of University of Freiburg.
22331
Reposted by Wolfgang Hess
jensrna.bsky.social @jensrna.bsky.social · 23/06/2026
New breakthrough in @NucleicAcidsRes! Transcriptional Interference (TI) protects Nostoc PCC7120🦠from toxic gene expression. This is the first independent proof that TI can be monitored in vivo via the rifi R-package. doi.org/10.1093/nar/..., doi.org/10.1038/s420...
doi.org
Antisense RNA controls the degradation of phycobilisomes in heterocyst-forming cyanobacteria by a transcriptional interference mechanism
Abstract. Transcriptional interference is rarely documented in bacteria. In cyanobacteria, phycobilisomes are the major light-harvesting antennae, and thei
041
Wolfgang Hess @cyanolab.bsky.social · 23/06/2026
NAR Breakthrough Article: Antisense RNA controls the degradation of phycobilisomes in heterocyst-forming cyanobacteria by a transcriptional interference mechanism: academic.oup.com/nar/article/...
021
Reposted by Wolfgang Hess
PLOS Biology @plosbiology.org · 18/02/2026
Accurate RNA structure prediction remains a challenge, despite recent computational advances. This study presents DRFold2, a #DeepLearning framework that significantly enhances accuracy of de novo #RNAstructure prediction by increasing contact prediction precision @plosbiology.org 🧪 plos.io/4aoOQiX
Top:  A representative modeling example from Chimpanzee CPEB3 HDV-like ribozyme (PDB ID: 7QR3), with models predicted by four better-performing methods (blue cartoons) overlaid on experimental structure (gray cartoons). Left to right: DRFold2, DeepFoldRNA, AlphaFold3, RhoFold. Bottom: Structural visualization of the example from coxsackievirus B3 cloverleaf RNA (PDBID: 8DP3), showing experimental structure (left), AlphaFold3’s best prediction from 100 models (middle), and 5th model of DRfold2 (right), respectively. Structures are rainbow-colored from 5′ (blue) to 3′ (red) end.
0259
Wolfgang Hess @cyanolab.bsky.social · 05/12/2025
A new + interesting paper on the guanidine riboswitch in Synechocystis and other cyanobacteria: "Deciphering guanidine assimilation and ri"boswitch-based gene regulation in cyanobacteria for synthetic biology applications" | PNAS www.pnas.org/doi/10.1073/...
pnas.org
Deciphering guanidine assimilation and riboswitch-based gene regulation in cyanobacteria for synthetic biology applications | PNAS
Guanidine is well known as a denaturing agent. However, recent studies have demonstrated both the widespread synthesis of guanidine, e.g., in plant...
040
Wolfgang Hess @cyanolab.bsky.social · 26/09/2025
Riding a train in China, returning from the ISPP 2025 and the Green Carbon conference.
The train station in Qingdao. A light-flooded palace for high-speed trains.Inside the high-speed train Qingdao-Beijing in China. Riding at more than 300 km/h.
070
Wolfgang Hess @cyanolab.bsky.social · 26/09/2025
„RAPDOR: Using Jensen-Shannon Distance for the computational analysis of complex proteomics dataset“ rdcu.be/eImcr : 11 authors from 3 labs. Many thanks to all for making this possible!
rdcu.be
RAPDOR: Using Jensen-Shannon Distance for the computational analysis of complex proteomics datasets
Nature Communications - RNA-binding proteins play key roles in post-transcriptional gene regulation. Here, Hemm et al. developed RAPDOR, a widely applicable tool based on Jensen-Shannon Distance...
062
Wolfgang Hess @cyanolab.bsky.social · 26/09/2025
To correctly determine a distance there, we used Jensen Shannon Divergence as a metric, which is a symmetrified version of the well-known Kullback–Leibler divergence for comparing distributions. The GradR data can be searched online at synecho-rapdor.biologie.uni-freiburg.de @meinbio.bsky.social
synecho-rapdor.biologie.uni-freiburg.de
Welcome
020
Wolfgang Hess @cyanolab.bsky.social · 26/09/2025
For the intuitive analysis + visualization of such datasets we introduce RAPDOR. In contrast to expression profiles, which are compared e.g. by correlation, the results of GradR and similar approaches are naturally displayed as distributions, as the protein fraction in each compartment is measured.
110
Wolfgang Hess @cyanolab.bsky.social · 26/09/2025
We have been searching for novel RNA-binding proteins and validated, among many candidates, Sll1967 (a possible RlmD homolog), Sll0726 (phosphoglucomutase), Ssl2245 (a putative antitoxin), Slr0711 (possible QueF homolog), and Sll0947 (ribosome-associated inhibitor RaiA/LrtA homolog) as binding RNA.
110
Wolfgang Hess @cyanolab.bsky.social · 26/09/2025
How to search for unknown RNA-binding proteins? We did GradR in the cyanobacterium Synechocystis 6803. And now our paper „RAPDOR: Using Jensen-Shannon Distance for the computational analysis of complex proteomics dataset“ is finally out in Nature Communications, here: rdcu.be/eImcr .
rdcu.be
RAPDOR: Using Jensen-Shannon Distance for the computational analysis of complex proteomics datasets
Nature Communications - RNA-binding proteins play key roles in post-transcriptional gene regulation. Here, Hemm et al. developed RAPDOR, a widely applicable tool based on Jensen-Shannon Distance...
142
Wolfgang Hess @cyanolab.bsky.social · 14/08/2025
Riding a train in Lithuania.
Figure on track 1.The train is made to look nice. Forest items everywhere
050
Wolfgang Hess @cyanolab.bsky.social · 26/06/2025
¡Muchas gracias, José!
010
Wolfgang Hess @cyanolab.bsky.social · 26/06/2025
Many thanks, Jörg! And it has been a pleasure to visit Göttingen recently!
010
Wolfgang Hess @cyanolab.bsky.social · 26/06/2025
RNA-binding proteins and photosynthesis: The RRM domain–containing protein Rbp3 interacts with ribosomes and the 3’ ends of mRNAs encoding photosynthesis proteins | PNAS www.pnas.org/doi/10.1073/...
273
Wolfgang Hess @cyanolab.bsky.social · 26/06/2025
Inorganic carbon levels regulate growth via SigC signaling cascade in cyanobacteria - Kurkela - New Phytologist - Wiley Online Library nph.onlinelibrary.wiley.com/doi/10.1111/...
nph.onlinelibrary.wiley.com
Inorganic carbon levels regulate growth via SigC signaling cascade in cyanobacteria
Cyanobacterial growth depends on inorganic carbon (Ci; CO2 and bicarbonate) concentration, but mechanism(s) adjusting photosynthesis and growth according to Ci remain unclear. ΔrpoZ cells lacking th...
062
Wolfgang Hess @cyanolab.bsky.social · 23/06/2025
Measuring carbon isotope discrimination and crucial gene expression changes indicated that C. braunii expresses a CO2-concentrating mechanism to cope with these challenges. Supported by @watertoland.bsky.social Details here: nph.onlinelibrary.wiley.com/doi/10.1111/...
nph.onlinelibrary.wiley.com
Evidence for a CO2‐concentrating mechanism in the model streptophyte green alga Chara braunii
Oxygenic photosynthesis in streptophytic algae, such as Charophyceae, is often impeded by low CO2 levels in aquatic habitats. Consequently, many algal groups evolved a CO2-concentrating mechanism (C...
0105
Wolfgang Hess @cyanolab.bsky.social · 23/06/2025
They must cope with low CO2 solubility in water, which can limit the CO2 fixation by RubisCO. Moreover, the total pool of inorganic carbon is highly variable in this environment, depending on pH, temperature, and salinity.
120
Wolfgang Hess @cyanolab.bsky.social · 23/06/2025
How are fully submerged algae efficiently fixing carbon? All organisms performing oxygenic photosynthesis fix CO2 in the Calvin–Benson–Bassham cycle using RubisCO. But aquatic organisms have a challenge.
140
Reposted by Wolfgang Hess
Daan C. Swarts @dcswarts.bsky.social · 13/05/2025
Out now: Cyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNA cell.com/molecular-ce... Most long-A pAgos interfere with invading DNA solo. Why then are cyanobacterial pAgos co-encoded with a Cas4-like protein?
13212
Reposted by Wolfgang Hess
Chitrang Dani @danichitrang.bsky.social · 18/03/2025
Just three days left for the deadline for oral abstract submissions for the 15th Workshop on Cyanobacteria at Vanderbilt University (Nashville, TN, USA) - don't miss out! Workshop dates: 4-7th June 2025. More information: web.cvent.com/event/3d0bd3.... Please share widely!
web.cvent.com
15th Workshop on Cyanobacteria. Online registration by Cvent
11110
Wolfgang Hess @cyanolab.bsky.social · 03/03/2025
TIGR-Tas: A new family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses: www.science.org/doi/10.1126/...
science.org
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses
RNA-guided systems provide remarkable versatility, enabling diverse biological functions. Through iterative structural and sequence homology-based mining starting with a guide RNA-interaction domain o...
010
Reposted by Wolfgang Hess
Nucleic Acids Research @narjournal.bsky.social · 27/02/2025
🚨 New #Breakthrough article from @holmqvist-lab.bsky.social: "ProQ prevents mRNA degradation through inhibition of poly(A) polymerase" #RNA #Stability #Degredation #mRNA 📖 Read the full article here: doi.org/10.1093/nar/gkaf103
0247
Wolfgang Hess @cyanolab.bsky.social · 06/02/2025
Our new Opinion article, now out in Trends in Plant Science is the result of a great collaboration with Conrad Mullineaux and Annegret Wilde freecyano.bsky.social @cyanolab.bsky.social
freecyano.bsky.social
Annegret Wilde (@freecyano.bsky.social)
cyanobacteriologist from University Freiburg
051
Wolfgang Hess @cyanolab.bsky.social · 06/02/2025
Why did chloroplasts retain some genes from their free-living ancestors rather than transferring all of them to the nucleus following endosymbiosis? We suggest that mRNA targeting might play a role. www.sciencedirect.com/science/arti...
sciencedirect.com
Does mRNA targeting explain gene retention in chloroplasts?
During their evolution from cyanobacteria, plastids have relinquished most of their genes to the host cell nucleus, but have retained a core set of ge…
1146
Reposted by Wolfgang Hess
Sonja-Verena Albers @archaellum.bsky.social · 03/02/2025
Would you expect that Bacteria use an archaellum for swimming? We didn't, but we found that some Chloroflexota do! Find the story here: www.biorxiv.org/content/10.1... A little thread below 1/n
biorxiv.org
Horizontal gene transfer of the functional archaellum machinery to Bacteria
Motility in Archaea is driven by a nanomachinery called the archaellum. So far, archaella have been exclusively described for the archaeal domain; however, a recent study reported the presence of arch...
112757
Reposted by Wolfgang Hess
ᐯIᑕTOᖇ ᑎIᘔET, ᗰᗪ @nizet.bsky.social · 25/01/2025
🅲🅾🅾🅻 🆃🅴🅲🅷🅽🅾🅻🅾🅶🆈 “Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
science.org
Highly multiplexed spatial transcriptomics in bacteria
Single-cell decisions made in complex environments underlie many bacterial phenomena. Image-based transcriptomics approaches offer an avenue to study such behaviors, yet these approaches have been hin...
19831
Wolfgang Hess @cyanolab.bsky.social · 26/01/2025
This review has been put together by 12 authors working on multicellularity in bacteria. Many thanks to the DFG by supporting this research through the SPP priority program 2389 “Emergent Functions of Bacterial Multicellularity”.
000
Wolfgang Hess @cyanolab.bsky.social · 26/01/2025
Multicellularity is a key feature of complex life, but how to study multicellularity in bacteria and at the single cell level? Use advanced microscopy, MS imaging, flow cytometry, spatial and single-cell transcriptomics, advanced microfluidics and more. See here: journals.asm.org/doi/10.1128/...
journals.asm.org
Resolving spatiotemporal dynamics in bacterial multicellular populations: approaches and challenges | Microbiology and Molecular Biology Reviews
SUMMARYThe development of multicellularity represents a key evolutionary transition that is crucial for the emergence of complex life forms. Although multicellularity has traditionally been studied in...
161
Reposted by Wolfgang Hess
CIBSS – Centre for Integrative Biological Signalling Studies @cibss.bsky.social · 10/01/2025
Mark your calendars for next Tuesday: Tel Aviv University's Prof. Eilon Shani will present a #CRISPR -based toolkit that targets multiple genes at a genome-wide scale, addressing functional overlap in genetic systems and to uncover hidden mechanisms behind hormone transport. kurzlinks.de/fprn
Sharepic showing Date, Time and Place of Prof. Eilon Shani's CIBSS Seminar talk on "A Multi-targeted genome-scale CRISPR Toolbox to Overcome Functional Redundancy and Reveal Hidden Hormone Transport Mechanisms", Tuesday, January 14th 2025, Lecture Hall, Institute of Biology I, Hauptstr. 1, and online per Zoom.
0116
Wolfgang Hess @cyanolab.bsky.social · 20/12/2024
The conformational space of RNase P RNA in solution using atomic force microscopy: www.nature.com/articles/s41...
nature.com
The conformational space of RNase P RNA in solution - Nature
Using a deep neural network and statistical analyses of atomic force microscopy images of individual RNA molecules enables the mapping of RNA conformational space in solution.
030
Wolfgang Hess @cyanolab.bsky.social · 19/12/2024
PatR (Alr1700) is a previously not characterized protein that binds sRNAs and functions as a master regulator of heterocyst patterning and differentiation. academic.oup.com/nar/advance-...
academic.oup.com
R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning
Abstract. RNA-binding proteins (RBPs) are central components of gene regulatory networks. The differentiation of heterocysts in filamentous cyanobacteria i
030
Wolfgang Hess @cyanolab.bsky.social · 19/12/2024
Not all bacteria, even of a single species, are created equal. Nor are they always unicellular. Some cyanobacteria develop heterocysts and fix nitrogen. GradR/R-DeeP analysis identified new RNA-binding proteins. Some were previously known for other functions. Others were completely uncharacterized.
150
Wolfgang Hess @cyanolab.bsky.social · 19/12/2024
Congratulations to our first author, Manuel, that the results are finally out! And we thank all our friends, colleagues and collaborators in Freiburg, Sevilla and Heidelberg. @embl.org @unisevilla.bsky.social @biologyunifreiburg.bsky.social
040
Wolfgang Hess @cyanolab.bsky.social · 19/12/2024
R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning. academic.oup.com/nar/advance-...
academic.oup.com
R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning
Abstract. RNA-binding proteins (RBPs) are central components of gene regulatory networks. The differentiation of heterocysts in filamentous cyanobacteria i
121
Reposted by Wolfgang Hess
KleineVehnLab @kleinevehnlab.bsky.social · 17/12/2024
We are looking for a new colleague :) Please spread the word!
11622
Wolfgang Hess @cyanolab.bsky.social · 14/12/2024
ISPP-18, the "International Symposium on Phototrophic Prokaryotes" will take place September 8-12, 2025. This is the best conference in the world to learn about recent research in photosynthetic prokaryotes. Only every 3 years. Registration for ISPP-18 is now open. ispp2025-qingdao.com
ispp2025-qingdao.com
0115
Wolfgang Hess @cyanolab.bsky.social · 13/12/2024
How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress: onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com
How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress
Cyanobacteria are powered by oxygenic photosynthesis located in extensive internal membrane systems, the thylakoids. Using carboxysomes, they assimilate organic from inorganic carbon. The integration...
0146
Reposted by Wolfgang Hess
Jens Hör @jenshoer.bsky.social · 12/12/2024
The structure of Zorya is mind-blowing 🙀 www.nature.com/articles/s41...
nature.com
Structure and mechanism of the Zorya anti-phage defense system - Nature
Nature - Structure and mechanism of the Zorya anti-phage defense system
075
Reposted by Wolfgang Hess
Cyanotrans @cyanotrans.bsky.social · 11/12/2024
Integration of horizontally acquired light-harvesting genes into an ancestral regulatory network in the cyanobacterium Acaryochloris marina MBIC11017 | mBio journals.asm.org/doi/10.1128/...
journals.asm.org
Integration of horizontally acquired light-harvesting genes into an ancestral regulatory network in the cyanobacterium Acaryochloris marina MBIC11017 | mBio
Horizontal gene transfer, the asymmetric movement of genetic information between donor and recipient organisms, is an important mechanism for acquiring new traits. In order for newly acquired gene content to be retained, it must be integrated into the ...
041
Reposted by Wolfgang Hess
Sophie Zoe Farkas @sophiezoe.bsky.social · 09/12/2024
I love this tradition at @biologyunifreiburg.bsky.social, every December the student representatives visit each floor and sing Christmas carols for us.
092
Wolfgang Hess @cyanolab.bsky.social · 08/12/2024
This work has been a great collaboration with the labs of Martin Hagemann in Rostock, Satoru Watanabe in Tokyo, and Roman Sobotka in Trebon. Many thanks!
000
Wolfgang Hess @cyanolab.bsky.social · 08/12/2024
Cytosine N4-methylation by the DNA methyltransferase M.Ssp6803II is required for the proper expression level of protoporphyrinogen IX oxidase, HemJ. All details in our new paper in DNA Research: academic.oup.com/dnaresearch/...
academic.oup.com
Epigenetic control of tetrapyrrole biosynthesis by m4C DNA methylation in a cyanobacterium
Abstract. Epigenetic DNA modifications are pivotal in eukaryotic gene expression, but their regulatory significance in bacteria is less understood. In Syne
165
Wolfgang Hess @cyanolab.bsky.social · 03/12/2024
Homologs of YlxR/Ssr1238 exist in all cyanobacteria, but also in many other bacteria. The protein in Bacillus subtilis has recently been characterized as a modulator of RNase P activity. Are there more secrets of RNase P? Here the paper by Jörg Stülke & his team: academic.oup.com/nar/article/...
academic.oup.com
The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis in vitro
Abstract. Even though Bacillus subtilis is one of the most studied organisms, no function has been identified for about 20% of its proteins. Among these un
010
Wolfgang Hess @cyanolab.bsky.social · 03/12/2024
The secrets of RNase P. This endoribonuclease is ubiquitous, but only in bacteria it is a ribozyme. We have characterized Ssr1238, a YlxR/DUF448 family protein to interact with the RNase P RNA in cyanobacteria. Details in our new paper: www.tandfonline.com/doi/full/10....
tandfonline.com
Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria
Throughout the tree of life RNA-binding proteins play important roles, but they are poorly characterized in cyanobacteria. Overexpression of the predicted RNA-binding protein Ssr1238 in the cyanoba...
143