biorxiv.org
MitoTracker transfers from astrocytes to neurons independently of mitochondria
The neuroprotective transfer of mitochondria from astrocytes to neurons has been primarily investigated by labelling astrocytic mitochondria with the dye MitoTracker. Here we report that MitoTracker transfers to neurons from both astrocytes and astrocyte-conditioned media, independently of mitochondrial transfer. Our observations should prompt an essential re-evaluation of the literature concerning astrocyte-neuron mitochondrial transfer and in other systems in which contact-independent transfer has been observed using mitochondrial dyes. ### Competing Interest Statement The authors have declared no competing interest. MSD (United Kingdom), https://ror.org/004nn4n27, PRJ_20446 DEVINE MSD MRC The Francis Crick Institute, https://ror.org/04tnbqb63, CC2206
![According to Brhaspati, thinking is due to the four constitutive
principles of matter, just as the power to intoxicate is due to the
ingredients in the wine. What we call a human body, or a sense
organ, or a physical object, is just a combination (samudāya; an as-
semblage) of earth, fire, air, and water; indeed, these four kinds of
matter are all there is. A person is a human body endued with think-
ing, and individual lives differ one from another as bubbles differ in
water. Recent work on the reconstruction of Br q haspati’s text allows us
to conjecture that it begins as follows:
1.1 Next then we will examine the nature of the reals.
1.2 Earth, fire, air, and water are the reals.
1.3 Their combination is called the ‘body’, ‘senses’, and ‘objects’.
1.4 Consciousness (caitanya) [is formed] out of these [elementary reals].
1.5 As the power to intoxicate [is formed] out of fermenting ingredients.
1.6 A human being (purus q a) is a body qualified by consciousness.
1.7 [Thinking is] from the body alone.
1.8 Because of its presence when there is a body.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:efplwzzow7blwi7kjetemmnn/bafkreib4gfye6g6yet645svqnompybqc2iwd6afuya5uvgcqik3n4wl7by)
