Sequencing of liver tissue from a patient treated with the rAAV gene therapy Zolgensma for spinal muscular atrophy recently revealed contaminating plasmid sequences derived from rAAV manufacturing wit...

Neuropsychopharmacology - Splice isoforms of the histone variant macroH2A1 differentially regulate hippocampal gene expression and memory formation

Pouget et al. identified distinct CA1 neuron ensembles active during specific moments of fear learning and uncovered the core engram essential for memory formation.

Pouget et al. identified distinct CA1 neuron ensembles active during specific moments of fear learning and uncovered the core engram essential for memory formation.

During recombinant adeno-associated virus (rAAV) production, certain components of the manufacturing system can be encapsidated as unwanted nucleic acid contaminants. Prior work has established that t...

During recombinant adeno-associated virus (rAAV) production, certain components of the manufacturing system can be encapsidated as unwanted nucleic acid contaminants. Prior work has established that the p5 promoter is critical for efficient vector production and is responsible for a significant portion of this aberrant cross-packaging. The Rep binding element (RBE) and terminal resolution site (TRS)-mimic loop on p5 putatively facilitate off-target packaging of DNA directly adjacent to p5 into rAAV particles.

How does the brain balance innate responses with adaptive learning when reacting to threats? This study shows that repeated exposure to looming stimuli reduces innate defensive responses via hippocamp...

TIRTL-seq is a high-throughput method for paired T cell receptor sequencing at the cohort scale.

α/β T cells are key players in adaptive immunity. The specificity of T cells is determined by the sequences of the hypervariable T cell receptor (TCR) α and β chains. Although bulk TCR sequencing offe...

Adeno-associated virus (AAV) has been in clinical use for gene therapy applications for over a decade, with 6 FDA-approved drug products at the time of writing.1 However, this vector is manufactured i...

Neuropsychopharmacology - Role of the histone variant H2A.Z.1 in memory, transcription, and alternative splicing is mediated by lysine modification