Reposted by Basil GreberDon Moynihan @donmoyn.bsky.social · 2hSelling out Ukraine to side with Russia in order to marginally reduce the negative impacts of his dumb war with Iran 21459113
Reposted by Basil GreberGerry Doyle @mgerrydoyle.bsky.social · 13hcredit where credit is due, this is straight ahead factual and well written 261728419
Reposted by Basil GreberKit Yates @kityates.bsky.social · 9hWho doesn't love a good spreadsheet error. Especially when it informs policy. Wonderful.theguardian.comTories overstate net zero cost by nearly £500bn in apparent spreadsheet errorExclusive: Badenoch’s flagship conference policy document cites £957bn estimate, twice the total in climate advisers’ data 1417
Reposted by Basil GreberVytaute Boreikaite @vytaute.bsky.social · 08/10/2026I’m very excited to share my postdoc work in the @plaschkalab.bsky.social, where we investigated the very final act of splicing – spliceosome termination, revealing the stepwise dismantling of the spent spliceosome www.nature.com/articles/s41... (1/11)nature.comMechanism of spliceosome termination - NatureA coordinated sequence of RNA helicases and associated factors dismantles normal and defective spliceosome complexes to enable intron degradation and ensure accurate gene expression. 27626
Reposted by Basil GrebercryoEM papers @cryoempapers.bsky.social · 06/10/2026Structural basis of PARP2 recognition of nucleosomal DNA breaks and inhibitor trapping pubmed.ncbi.nlm.nih.gov/42831429/ #cryoEM 032
Reposted by Basil GreberAlex Merz 🇺🇸🇨🇦🇺🇦 @merz.bsky.social · 05/10/2026"Photograph 51 was not the result of an experiment in need of analysis, but was refined documentation for calculations that she had already performed on the earlier photograph." link.springer.com/content/pdf/... 33518
Basil Greber @bjgreber.bsky.social · 05/10/2026Travelling through Lisbon without having a go at taking a photo of Vasco da Gama Bridge at sunrise? Impossible! 0130
Basil Greber @bjgreber.bsky.social · 05/10/2026Also happened when I applied for faculty positions in Europe, and as far as I can tell it still happens. 010
Reposted by Basil GreberTim Carmody @tcarmody.bsky.social · 02/10/2026This is the funniest thing I've seen today, possibly ever 41120453122
Reposted by Basil GreberJulian Sale @juliansalelab.bsky.social · 02/10/2026Congratulations Tanmay, on receiving the @embo.org Gold Medal! @mrclmb.ac.uk @embl.org @dunnschool.bsky.social 2394
Basil Greber @bjgreber.bsky.social · 01/10/2026BH is the one we use most, yes. We did combine datasets but did not mix peptides. That is certainly another way to tailor the “interface coating” to your needs. 010
Basil Greber @bjgreber.bsky.social · 01/10/2026... and they do not reduce particle numbers in the way many detergents do. Have a look at our examples, and give it a shot if you think it might help with your specimens! 130
Basil Greber @bjgreber.bsky.social · 01/10/2026We do not claim that cryo-tides will solve all your #cryoEM sample prep issues, but it certainly helped us in several instances. The advantage of this approach is that it is trivial to obtain the peptides, they induce negligible background and are compatible with small targets... 130
Basil Greber @bjgreber.bsky.social · 01/10/2026During this time, @amymcgeoch.bsky.social in my lab started working with the CDK11 complex, which showed strong preferred orientation. Addition of the original (BH) cryo-tide induced additional particle views, which eventually helped us determine the 2.3 Å-resolution structure of the complex. 151
Basil Greber @bjgreber.bsky.social · 01/10/2026Strikingly, @claudioalfieri.bsky.social and his team demonstrate that using cryo-tides, they can obtain high-resolution structures of APC/C, which otherwise requires carbon support. 162
Basil Greber @bjgreber.bsky.social · 01/10/2026We show that the addition of synthetic peptides - which we refer to as cryo-tides - to #cryoEM samples can protect them from denaturation and can mitigate preferred orientation issues. 131
Basil Greber @bjgreber.bsky.social · 01/10/2026... check out the latest pre-print by @claudioalfieri.bsky.social & his team! www.biorxiv.org/content/10.6...biorxiv.orgCryo-tides: synthetic peptides for cryo-EM grid preparation of multi-subunit protein complexes sensitive to the air-water interfaceCryogenic electron microscopy (cryo-EM) has enabled rapid progress in structural biology throughout the last decade. However, cryo-EM sample preparation still represents a major bottleneck in high-res... 1195
Basil Greber @bjgreber.bsky.social · 01/10/2026Those who had a careful look at the methods section of our work on the #cryoEM structure of the CDK11-cyclin L-SAP30BP complex will have noticed that we added a short peptide to our sample before freezing. If you wonder why... 14220
Basil Greber @bjgreber.bsky.social · 01/10/2026Thanks! But it is actually work from @claudioalfieri.bsky.social’s lab. Due to some quirk, bioRxiv lists him as first author rather than last. 010
Reposted by Basil GreberCarl T. Bergstrom @carlbergstrom.com · 01/10/2026"An ambulance was seen arriving at the prison where Pike was to be executed and then leaving. Medical aid was being administered, according to online federal court records." The utter absurdity of the state trying twice to kill someone and then calling an ambulance. We must end the death penalty.nbcnews.comTennessee inmate still alive after being administered two lethal injections, her lawyer saysChrista Pike, 50, was 18 when she murdered a classmate. She would be the first woman the state has executed in more than 200 years. 25647158
Reposted by Basil GreberMelanie D. White @melaniedwhite.bsky.social · 01/10/2026I spoke with @nature.com about the Nikon Small World controversy. As AI becomes part of scientific imaging, we need greater transparency about what was measured, inferred or generated. It's not about excluding AI. It’s about preserving trust in scientific evidence. www.nature.com/articles/d41...nature.comThis award-winning microscopy image used AI — igniting controversy in a prestigious competitionResearchers say that using artificial-intelligence tools to visualize scientific images can become problematic when models misrepresent the underlying data. 44514
Basil Greber @bjgreber.bsky.social · 28/09/2026This further highlights the risks of using tools that were created by companies who were, well, "not very selective" with how they sourced the information required to develop their models. It is becoming increasingly unlikely that users of these tools are being treated any differently. 072
Reposted by Basil Greberc0nc0rdance @c0nc0rdance.bsky.social · 27/09/2026The obvious conclusion (to me): if you have proprietary research & you upload it into the Plagiarism Nexus, it's not YOUR proprietary research anymore. Behave accordingly. 728193
Reposted by Basil GreberCarl T. Bergstrom @carlbergstrom.com · 27/09/2026Ignore for a moment the question about whether Anthropic trains on user sessions. I am I right in reading this as saying that Anthropic's "never-before-discovered" reverse transcriptase was *patented* three years ago? 10380112
Reposted by Basil GreberMicah @rincewind.run · 26/09/2026I think it is very important that Big Balls, personally, goes to jail there are a lot of young goons who think they are invincible and it is important to make it clear that they are not 7065721333
Reposted by Basil GreberMike Masnick @masnick.com · 26/09/2026Yes, yes, I'm sure that will stop all interest in the film. 1346163
Reposted by Basil GreberSebastian S. Cocioba @atinygreencell.bsky.social · 25/09/2026IPTG >>> ChatGPT More wetlab for everyone! 2201
Reposted by Basil GreberCraig Kaplan @triggerloop.bsky.social · 25/09/2026Cryo-EM peeps are living the dream www.science.org/doi/10.1126/...science.orgIn-cell structures visualize human pre-ribosome assembly in the nucleolusIn eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly p... 16732
Reposted by Basil GreberArnaud Vanden Broeck @avbroeck.bsky.social · 23/09/2026Very happy to share the first paper from our lab, out now in Nature Communications!🎉🥳 Structural basis of step II Spliced Leader RNA trans-splicing in trypanosomatid parasites 🧬🔬 🔗 www.nature.com/articles/s41... @natcomms.nature.com @universitedeliege.bsky.social @rockefeller.edu #cryoEM #cryo-EM 33410
Reposted by Basil GreberMaya Voichek @mayavoichek.bsky.social · 24/09/2026Super excited to share my postdoctoral work at @imbavienna.bsky.social @viennabiocenter.bsky.social - We discovered that some retrotransposons, or "jumping genes" 🧬, are able to spread from cell to cell via a new viral infectivity route. A short thread: 🧵👇 (1/7) 20258148
Reposted by Basil GreberBeijingPalmer @beijingpalmer.bsky.social · 23/09/2026the priority here being the migrants and not the vigilantes is *insane* and also more evidence of my Belphegor theory. 744168
Reposted by Basil GreberSwiss National Science Foundation (SNSF) @snsf.ch · 22/09/2026🏆 Biochemist Martin Jinek is the winner of the Swiss Science Prize #MarcelBenoist 2026, considered the Swiss #Nobel Prize. He contributed to the development of molecular scissors, namely CRISPR-Cas9 technology. Read more ➡️ sohub.io/3hs4 66321
Reposted by Basil GreberChristophe 🔬 Leterrier @christlet.bsky.social · 22/09/2026This is the updated part. Looks like the movie is an AI-generated fluorescence image from a non-labeled acquisition technique. No indication that the Small World jury and organizers have requested and verified the raw data (as mentioned as a possibility in the competition rules) 155023
Reposted by Basil GreberRichard Sever @richardsever.bsky.social · 27/08/2026$3.7 billion spent on APCs in 2025. That's ~1000X more than it costs to run bioRxiv/medRxiv. Worth thinking about as we consider how to fund infrastructure... arxiv.org/pdf/2608.16322arxiv.org 813379
Reposted by Basil GreberYuan He @yuanhejhu.bsky.social · 22/09/2026A big week for the lab! Two new papers on how cells repair DNA double-strand breaks: one on getting NHEJ started in chromatin, one on processing the ends before ligation. Years of work by a fantastic team. 410940
Basil Greber @bjgreber.bsky.social · 21/09/2026Maybe pressure Putin to leave Ukraine alone. How about that? 040
Basil Greber @bjgreber.bsky.social · 19/09/2026That may be the case, yes. I suspect some journals might be replacing all instances of movie with video when they create the proofs. I have had to undo such changes in some of my papers. 021
Reposted by Basil GreberProf. Stefan Rahmstorf @rahmstorf.bsky.social · 19/09/2026Sommer 2026: 16.000 Hitzetote 🥵 alleine in 🇩🇪. Dürre. Brände. 🤯 Die Bundesregierung subventioniert jetzt mit 2.500.000.000 € von unseren Steuergeldern die Ursache der Hitze: fossile Brennstoffe. Unfassbar. 🤯🤯🤯 Das meiste Geld landet dann wieder bei den Ölkonzernen, der Lobby gegen Klimaschutz. 832119932
Basil Greber @bjgreber.bsky.social · 18/09/2026I have now read a fair few #cryoEM papers in which authors write "raw videos" instead of "raw movies" when referring to dose-fractionated exposures. I know that journal editors/copyediting staff often suggest this change. But nobody in the field says "videos", so maybe journals need to be told that? 1110
Basil Greber @bjgreber.bsky.social · 18/09/2026They openly disclose it in the figure legend, so seems ok. Also, for those wondering why the two cells in panels b, c look the same, it is because it is the same cell imaged with different methods. 010
Basil Greber @bjgreber.bsky.social · 18/09/2026More could have been done, with more urgency, to prevent this calamity by legal means, but this did not happen. So, to reiterate, if you believe that everything will be different this time around, I hope you are right. All of that said, I apologise for the flippant wording of my initial response. 000
Basil Greber @bjgreber.bsky.social · 18/09/2026There is well-known precedent for a criminal president being pardoned for the sake of “ending divisions” (though admittedly not by a Dem president). As for the rest, people spent years giddily posting about impending accountability (Muller! Impeachment! Garland! Smith!), but we got Trump II instead. 100
Basil Greber @bjgreber.bsky.social · 17/09/2026Jack Smith’s cases were dismissed because Trump managed to run out the clock after it took almost two years until a special counsel was appointed. My view that this was too long & that it was stupid to bring the classified documents case in FL was common at the time. Not sure how this is “bizarre”. 110