iiinova — where your digital self keeps evolving

At a time when centralised public scientific services cannot always be taken for granted, new efforts of this kind are worth paying attention to as part of a broader discussion about resilience, continuity and future development in open science infrastructure langtaosha.org.cn/lts/en/about

langtaosha.org.cn

020

So, overall, it will incorporate new technologies, and provide long term support and timely response to the growing needs of the international research community for science exchange 3/4

100

Langtaosha is not intended as another preprint server, but as platform that could develop across broad part of the research lifecycle including protocols, negative results, open commenting and AI-assisted review. The idea is to contribute to stable and transparent ecosystem for research exchange 2/4

100

Since our preprint on the ETC-ATP synthase supercomplex has attracted attention, I would like to introduce the platform we used, Langtaosha. It was launched by SMART together with several other institutions with support from Open Life Science Alliance, aiming for long-term open infrastructure 1/4

183

Happy to contribute to this fantastic collaboration. Bravo to students Mengchen Wu @mengchenwu.bsky.social and Yiqi Hu @huyiqi.bsky.social for leading these projects. Huge thanks to Yue Liu and his lab for tomo and all the collaborators!

020

High-res cryo-EM structures of different pieces from such long-range array in Leishmania provide details of the interfaces involved. The findings validate the single particle prequel and shed light on the divergent bioenergetic and cristae shaping strategies employed by different discobids. 3/4

110

In situ, the ETC–ATP synthase supercomplexes sit at both ends of ATP synthase rows. Units of (CIICIV2CV)(CV2)4(CIICIV2CV) are formed to tile the discoidal crista rim with defined register. With the help of cryo-ET and STA, molecular interactions scale up into a repeating membrane architecture. 2/4

100

In a followup with @longzhou88.bsky.social and Yue Liu we move from molecular mechanism to membrane architecture. A giant ETC-ATP synthase assembly containing 10 ATP synthases and 6 respiratory complexes builds long-range periodic OXPHOS arrays on native cristae langtaosha.org.cn/lts/en/prepr... 1/4

26613

Conceptually, this work extends Mitchell’s chemiosmotic theory. Beyond establishing proton motive force, oxidative phosphorylation depends on the geometry of the hosting membrane and the supramolecular organization that make the proton circuit optimal 6/6

060

Evolutionary analysis by Martijn Huynen further suggests that this architecture emerged in lineages where canonical complex I was lost or extensively modified, pointing to a new solution for spatial organization of oxidative phosphorylation 5/6

131

Biochemical, functional, and EM analyses by the Ondřej Gahura and Hassan Hashimi labs showed that disrupting the unique g′/g″ module selectively destabilizes the supercomplex, reshapes mitochondrial cristae, and lowers maximal membrane polarization capacity 4/6

120

Atomistic simulations by Vivek Sharma, showed induction of a shallow positive membrane curvature. By contrast, CIICIV₂ assembly lacking ATP synthase relaxed toward a flatter membrane conformation, suggesting that stable curvature is specifically sustained by incorporation of ATP synthase 3/6

110

CryoEM revealed 2MDa respiratory chain-ATP synthase supercomplex, organized around ATP synthase monomer together with complex II, dimer of complex IV A key organizing principle is a non-canonical subunit g′/g″ module repurposing ATP synthase dimerization interface to couple the respiratory chain 2/6

194