Nature Protocols - An approach for combining expansion microscopy with fluctuation-based super-resolution analysis enables the visualization of individual proteins via conventional fluorescence...

An approach for combining expansion microscopy with fluctuation-based super-resolution analysis enables the visualization of individual proteins via conventional fluorescence microscopy.

Expansion microscopy (ExM) is advancing, but lacks consensus on validation structures. Microtubules are an ideal ExM validation tool, due to their size, which is optimal for high-resolution techniques...

Combining expansion microscopy with super-resolution radial fluctuations captures the morphology of single proteins.

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Stretching protein samples in all directions pulls molecules farther apart, allowing them to be visualized using only light microscopy.

From cells to amino acids: 1000ExM pushes expansion microscopy beyond 1000×, potentially resolving individual protein residues on standard microscopes. Bigger volume expansion opens a new frontier for...

𝗘𝘅𝗽𝗮𝗻𝘀𝗶𝗼𝗻 𝗺𝗶𝗰𝗿𝗼𝘀𝗰𝗼𝗽𝘆 physically enlarges the sample, but it also dilutes fluorophores, exposes them to harsh chemistry, and leaves them in a water-rich gel. Not every dye survives that. Ali Shaib and...

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