iiinova — where your digital self keeps evolving

Reposted by Aisha Syeda

Aga Gambus @agagambus.bsky.social · 21/08/2026

New ERC Co-fund MOBILITY fellowships at University of Birmingham, UK. 6 outstanding postdoctoral researchers will be awarded 5-year fellowships to develop their independent future career. All within the umbrella of Molecular Biodiscovery. Details at: www.birmingham.ac.uk/mobility Please share!

birmingham.ac.uk

MOBILITY - University of Birmingham

Retaining Europe's brightest researchers to drive the next generation of life science breakthroughs.

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STCmicrobeblog @stcmicrobeblog.bsky.social · 16/08/2026

#MicroSky how to put GyrB into turbo mode...

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

I thoroughly enjoyed working with the awesome team at York including @bpsiyork.bsky.social , @agnesnoy.bsky.social , @alexpaynedwyer.bsky.social , @jackinatent.bsky.social , Katy Hollands, Lewis Frame, and collaborators at Durham, John Innes Centre, Inspiralis.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

Gyrase is an attractive antimicrobial target. Thus, this new revelation about the behaviour of this enzyme in vivo opens a new angle for developing novel antimicrobials.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

Our observation of a condensate boosting catalytic throughput of an essential enzyme, reveals a mechanism that could explain activities of many enzymes well beyond gyrase.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

We propose that gyrase condensates keep the local gyrase concentration high to facilitate rapid turnover and catalysis at regions of high positive supercoils such as the replication fork. This is the answer to the longstanding question about gyrase processivity in its native cellular environment.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

Structural docking analysis suggests that excess GyrB facilities condensate formation via weak, multivalent interactions between gyrase subunits.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

We treated the cells with gyrase-targeting antibiotics and found that both copy number abundance and the in-vivo dynamics of either or both subunits change upon antibiotic treatment, further allowing us to understand how these antibiotics perturb gyrase function.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

We were in for a bigger surprise when we found that gyrase clusters contain ~150% more GyrB than GyrA, which is well beyond the whole-cell copy number ratio. Our FRAP experiments revealed that these clusters behave like liquid droplets.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

To our suprise we found that cells make ~40% more GyrB than GyrA. Not an equal stoichiometry as expected for the enzyme that was shown to have equal number of both subunits in the functional assembly. GyrB formed clusters as was observed for GyrA in a previous study (Stracy et al 2019)

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

We used high-speed single-molecule imaging to track fluorescently tagged gyrase subunits, GyrA and GyrB, in live E. coli, in real time, in their native cellular context.

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Aisha Syeda @aishasyeda.bsky.social · 16/08/2026

DNA gyrase is an essential enzyme that helps resolve positive supercoils. In vitro estimates indicate that a single gyrase enzyme resolves 1 supercoil/second. So how does it then keep up with replication in living cells that generate ~100 supercoils every second? We think we found why. 🧵

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Michael Eisen @mbeisen.bsky.social · 08/08/2026

need a favor from someone in Sweden

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Physics World @physicsworld.bsky.social · 07/08/2026

Two theoretical physicists – including a Nobel laureate – have solved a mathematical problem that flummoxed them for 10 years with help from Claude, the large language model developed by the US-based #AI firm Anthropic. 🧪⚛️ physicsworld.com/a/ai-model-h...

physicsworld.com

AI model helps physics Nobel laureate out of a decade-old mathematical jam – Physics World

Artificial intelligence is radically changing how researchers in some disciplines work

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Reposted by Aisha Syeda

bioRxivpreprint @biorxivpreprint.bsky.social · 23/06/2026

Single-molecule insights into DNA gyrase in live bacteria www.biorxiv.org/content/10.64898/20…

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Reposted by Aisha Syeda

bioRxiv Biophysics @biorxiv-biophys.bsky.social · 23/06/2026

Single-molecule insights into DNA gyrase in live bacteria www.biorxiv.org/content/10.64898/20…

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