zack chiang @zchiang.bsky.social · 11/11/20247 years ago, I met a junior fellow named Jason Buenrostro who blew me away with a vision of futuristic genomic technologies Today, we (Ajay Labade, Caroline Comenho) are excited to share our first steps into that future: Expansion in situ genome sequencing 1/ 714357
zack chiang @zchiang.bsky.social · 11/11/2024In 2020, we (Andrew Payne, Paul Reginato) showed in situ genome sequencing, which we used to reveal the 3D genome at the very first stages of life: www.science.org/doi/10.1126/... However, the resolution of IGS is capped by nuclear volume and the diffraction limit of light 2/science.orgIn situ genome sequencing resolves DNA sequence and structure in intact biological samplesA technical approach allows simultaneous sequencing and imaging of genomes in human fibroblasts and early mouse embryos. 190
Michelle Franc Ragsac, PhD 🇵🇭🇺🇸 @mragsac.com · 14/11/2024@zchiang.bsky.social ! Wonderful to see you on here ✨ Tagging this post for you to get more visibility on the science 🧪 and computational biology 💻🧬 feeds 🥹 #SciSky folx should definitely check out this #Genomics work! 🙌🏽 021
George Campbell @geobellward.bsky.social · 14/11/2024This project looks really awesome. For my own work, I am interested in how you characterized the re-embedding gel with fluorescence in Fig S1. What fluorescent reagent was used to incorporate into the gel? I may have overlooked it in the methods. Cheers. 100