zack chiang @zchiang.bsky.social · 11/11/20247 years ago, I met a junior fellow named Jason Buenrostro who blew me away with a vision of futuristic genomic technologies Today, we (Ajay Labade, Caroline Comenho) are excited to share our first steps into that future: Expansion in situ genome sequencing 1/ 714357
zack chiang @zchiang.bsky.social · 11/11/2024In 2020, we (Andrew Payne, Paul Reginato) showed in situ genome sequencing, which we used to reveal the 3D genome at the very first stages of life: www.science.org/doi/10.1126/... However, the resolution of IGS is capped by nuclear volume and the diffraction limit of light 2/science.orgIn situ genome sequencing resolves DNA sequence and structure in intact biological samplesA technical approach allows simultaneous sequencing and imaging of genomes in human fibroblasts and early mouse embryos. 190
zack chiang @zchiang.bsky.social · 11/11/2024Fortunately, we had Fei Chen & Ed Boyden, the inventors of expansion microscopy on board! In ExM, samples are physically enlarged in gels, allowing superresolution imaging without fancy microscopes 3/ 1130