Here's another one we've been sitting on for too long. I hope this helps prevent people from wasting increasingly precious time and money. I'm also very eager to hear rebuttals.
www.biorxiv.org/content/10.6...
biorxiv.org
Combinatorial CRISPR/Cas12a excises targeted loci in the mammalian genome at very low efficiency
The CRISPR/Cas12a system has emerged as a highly useful tool for genomic editing due to its ability to process multiple guide RNAs (gRNAs) from a single promoter. In this study, we explored the potent...
![CRISPR as a microbial immune system
In 2003, Mojica wrote the first paper suggesting that CRISPR was an innate microbial immune system. The paper was rejected by a series of high-profile journals, including Nature, Proceedings of the National Academy of Sciences, Molecular Microbiology and Nucleic Acids Research, before finally being accepted by Journal of Molecular Evolution in February, 2005.[3][4]](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:73aicoale2mqwrap63rdzwep/bafkreibu3dburfn527r3sbu2ov6y7gebv6zopaznagnfytjtsn6y4k7cs4)
