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tirthabanerjee.bsky.social

@tirthabanerjee.bsky.social
19 followers 19 following 13 posts
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 28/09/2026
My first PCB designed for neuro behavioural assays:D
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 14/07/2026
Our RAMFISH update is live: an open-source spatial transcriptomics ecosystem. Mid-plex (>30 genes) in 2D and whole mount tissues. Aligners for free-floating tissue. Quantification and visual confirmation. Preprint: www.biorxiv.org/content/10.1... GitHub: github.com/tdblab/RAMFISH
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 23/01/2026
Evolution rarely invents from scratch. Our recent study reveals that butterfly eyespots evolved by co-opting an ancient wing vein gene network. New trait, old genes. The study is now published in Communications Biology (featured: Jan26) www.nature.com/articles/s42...
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Shen Tian 田申 @tianshenbio.bsky.social · 24/10/2025
My main PhD work @monteirolab.bsky.social is now in @natecoevo.nature.com! We found a Hox gene promoter that helps butterflies🦋adjust their wing eyespots in response to seasonal temperatures🍃🍂, shedding light on the evolutionary origin of phenotypic plasticity. 1/9 www.nature.com/articles/s41...
Cr. to Shen Tian
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 19/10/2025
Happy to share that the preprint of our updated spatial mRNA multiplexing using RAMFISH (v2.0) is out... www.biorxiv.org/content/10.1... Special thanks to all the collaborators for their efforts.
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 17/08/2025
Excited to share that our Geneplexer and Multiplexer systems can now achieve spatial mRNA signal detection within 4.5 hrs, including hybridization, secondary amplification, and washes. Image: A z-slice (~5 µm) of a whole 14 dpf WT zf larva stained against markers nrp1a, gad2, and neuroD.
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 16/07/2025
Happy to collaborate with Ajay Mathuru's team at NUS. "Role of chrna5 in multi-substance preference and phenotypes comorbid with the development of substance dependence." Congratulations to the co-first authors, Tanisha Goel and Joshua Raine. www.biorxiv.org/content/10.1...
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Shen Tian 田申 @tianshenbio.bsky.social · 24/05/2025
Glad to share the final piece of my PhD thesis. We discovered a novel Hox gene promotor that might have facilitated the evolution of phenotypic plasticity across a butterfly subfamily. www.biorxiv.org/content/10.1...
biorxiv.org
A novel Hox gene promoter fuels the evolution of adaptive phenotypic plasticity
Adaptive phenotypic plasticity allows organisms to display distinct phenotypes in response to variable environments, but little is known about the genomic changes that promote the evolution of plastic...
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 10/04/2025
Happy to share that our recent Bicyclus larval brain multiplex-FISH paper has been featured on the cover of Methods and Protocols. www.mdpi.com/2409-9279/8/2
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Arnaud Martin @evolvwing.bsky.social · 05/04/2025
Preprint! Minos-mediated transgenesis in the pantry moth www.biorxiv.org/content/10.1... By @donyaniyaz.bsky.social
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 05/04/2025
Sharing a user friendly online app (beta phase) to design probes for HCR3.0 tdblab.github.io/hcrprobedesi...
tdblab.github.io
HCR Probe Designer
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 14/03/2025
Happy to share our latest 3D whole-mount multiplex-RNA brain scans of the larval brains of Bicyclus anynana at single-cell resolution... We mapped multiple targets at single-cell resolution and made the raw data open source for anyone who would like to analyse it. www.mdpi.com/2409-9279/8/...
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Arnaud Martin @evolvwing.bsky.social · 17/12/2024
New preprint by butterfly wizard @lucalivraghi.bsky.social www.biorxiv.org/content/10.1... We mapped optix (again?!) as the switch gene of a natural polymorphism, this time controlling silver patches of a mountain butterfly. Gorgeous RNAi validation, evidence of selective sweeps, introgression
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 12/12/2024
Our new manuscript describing gene multiplexing (using RAM-FISH) is finally out... We profiled multiple known and novel mRNA candidates during different developmental stages of Bicyclus and zebrafish using the easy-to-use HCR-based protocol that most labs can adapt. www.biorxiv.org/content/10.1...
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tirthabanerjee.bsky.social @tirthabanerjee.bsky.social · 05/12/2024
Happy to be a part of the work by @tianshenbio.bsky.social published @science.org Excellent work and many more to come🎉
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Shen Tian 田申 @tianshenbio.bsky.social · 05/12/2024
Our miRNA story is now in @science.org ! We found a microRNA, not a protein, that finally solved a long-standing evolutionary mystery of wing coloration in butterflies and moths. (1/n) www.science.org/doi/10.1126/...
science.org
A microRNA is the effector gene of a classic evolutionary hotspot locus
In Lepidoptera (butterflies and moths), the genomic region around the gene cortex is a “hotspot” locus, repeatedly implicated in generating intraspecific melanic wing color polymorphisms across 100 mi...
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