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Robin Journot 🔬| 🎾

@robinjournot.bsky.social
411 followers 134 following 35 posts

EMBO postdoc fellow at Institute of Human Biology, Basel. Liver organoids and niche perturbation. Alumni Institut Curie, AgroParisTech & ENS Ulm

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Reposted by Robin Journot 🔬| 🎾
Louise Dagher @louise-dagher.bsky.social · 13/10/2026
From my M1 internship to my PhD thesis viva, it’s been quite a journey! 👩🏻‍🔬🎉 I’m excited to share our new preprint! An interdisciplinary adventure, spanning microfluidics, biophysics, machine learning and modelling, to explore the mechanisms underlying the expansion of the first lumen.🔬 👩🏻‍💻 Check it out
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Reposted by Robin Journot 🔬| 🎾
Silvia Fre lab @frelab.bsky.social · 01/07/2026
So happy to be part of this distinguished community! Thank you to all colleagues @embo.org who voted for me!
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Silvia Fre lab @frelab.bsky.social · 26/06/2026
Week-end reading? Check out our new review on development and regeneration of glandular epithelia! With two fantastic co-authors: @robinjournot.bsky.social and @candice-merle.bsky.social ! 🤗 authors.elsevier.com/sd/article/S...
authors.elsevier.com
Shared molecular and cellular programs during regeneration of glandular epithelia
An emerging concept across multiple adult tissues implies that regeneration involves the transient reactivation of developmental programs, rather than…
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 26/06/2026
I’m happy to receive this prize, and I thank @gensocuk.bsky.social for the invitation. This achievement belongs to the entire @frelab.bsky.social at @institutcurie.bsky.social Grateful to all team members, collaborators and funding bodies who made it happen!
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 26/01/2026
Glad to receive a PhD prize from the @gensocuk.bsky.social for the work I lead on the origin and diversification of stratified epithelia. Thanks to @frelab.bsky.social , the lab, and all collaborators involved. Special thanks to @candice-merle.bsky.social for her help throughout the project.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 14/01/2026
I recently moved to Basel and was awarded an @embo.org Postdoctoral Fellowship to join the lab of Matthias Lutolf at the Institute of Human Biology. I will use data-driven approaches to investigate how micro-environmental cues shape human epithelial organoid maturation.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 14/01/2026
Many thanks to @frelab.bsky.social, all collaborators, interns, and researchers involved in these five years!
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 14/01/2026
My time at @institutcurie.bsky.social is done! Over the past months, I completed my PhD, published my first first-author paper in DevCell (www.cell.com/developmenta..., and submitted my first last-author manuscript to bioRxiv (www.biorxiv.org/content/10.1...).
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Reposted by Robin Journot 🔬| 🎾
Zev Gartner @zevgartner.bsky.social · 13/01/2026
1. Excited to share CONCORD, out in @NatureBiotech, an ML framework for single-cell analysis addressing integration, dimensionality reduction, and denoising in one go by @qinzhu1.🔗 www.nature.com/articles/s41... Check out this CONCORD model of worm development resolving differentiation trajectories:
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Alex Hughes Lab @hugheslabpenn.bsky.social · 13/12/2025
*New preprint alert* uncovering a mechanical pacemaker that synchronizes nephron formation with branching of the kidney's epithelial tubule tree. Read below to learn about this twisty journey lead by Sam Grindel and Sachin Davis in the lab. [Movie by Nils Lindstrom] www.biorxiv.org/content/10.1...
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Silvia Fre lab @frelab.bsky.social · 03/12/2025
Check out our beautiful story on tumour clonal dynamics with barcodes, performed in the @frelab.bsky.social by my super talented post-doc @candice-merle.bsky.social and @ivanditerlizzi.bsky.social from @rulands.bsky.social lab! #barcodes #plasticity
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Hao Yin @haoyin.bsky.social · 03/12/2025
An amazing preprint on molecular evolution (p63-Notch) of Epithelial Multilayering Cross-organ (14 in🐭), Cross-species (7🪰🐟x2🐸🐥🐭👤) single-cell transcriptomics p63+Jag2+ basal signal sendor▶️ p63-Hes1+ suprabasal receivers🐭 bioRxiv 2025 www.biorxiv.org/content/10.1...
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
Supported by: @agencerecherche.bsky.social @frm-officiel.bsky.social @fondationarc.bsky.social @cerclefser.bsky.social @worldwidecancer.bsky.social @institutcurie.bsky.social
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
This work was conducted at @institutcurie.bsky.social in @frelab.bsky.social. Thank you for supporting my work over the last five years! Big thank also to Candice Merle, the first author of this story, for her help in the experimental work and to our collaborators at @lbmcinlyon.bsky.social.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
Our work unifies decades of organ-specific studies into a single evo-devo framework. It argues for moving beyond organ-centric approaches toward cross-tissue comparisons to reveal conserved mechanisms of epithelial development, homeostasis, and disease. www.biorxiv.org/content/10.1...
biorxiv.org
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
Taken together, multilayered epithelia development follows a spatial and evolutionary hourglass: basal layers reactivate an ancestral ectodermal program, while suprabasal compartments diversify through modular, lineage-specific innovations.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
Lineage-tracing experiments across all 14 tissues show that activating Notch in basal cells consistently triggers suprabasal commitment, confirming the universality of this regulatory connection in stratified tissues.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
Basal and suprabasal layers are linked by a conserved p63–Notch axis. p63 maintains basal identity and induces Notch ligands, whereas Notch signaling drives suprabasal commitment.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
In contrast, suprabasal compartments show strong enrichment for tissue-specific and architecture-specific transcriptional modules. Functional diversification emerges primarily in suprabasal layers and follow a Russian-doll organization.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
This basal program has been repeatedly redeployed (heterotopy) in endoderm- and mesoderm-derived epithelia, allowing these tissues to initiate multilayering outside their original lineage of origin.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
We find that this conserved GRN is evolutionarily compartmentalized. Basal cells consistently deploy an ancestral ectodermal regulatory module centered on p63.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
Comparative analyses across lamprey, zebrafish, xenopus, chicken, mouse, and human reveal that multilayered epithelia rely on a deeply conserved set of genes. This suggests that the molecular foundations of epithelial stratification were established early in vertebrate evolution.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
Multilayered epithelia emerge from ectoderm, endoderm, and mesoderm, yet all adopt the same basic architecture: a basal compartment supporting one or more differentiated suprabasal layers. Do these different organs use distinct mechanisms, or a shared regulatory program?
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 17/11/2025
🚨 New preprint! We built a single-cell atlas of 14 multilayered epithelia and revealed a conserved transcriptomic program guiding tissue architecture and fate composition. Our work brings decades of tissue-specific studies together into a unified evo-devo framework. www.biorxiv.org/content/10.1...
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 16/11/2025
Supported by: @agencerecherche.bsky.social @frm-officiel.bsky.social @fondationarc.bsky.social @cerclefser.bsky.social @worldwidecancer.bsky.social @institutcurie.bsky.social
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Bardin Lab @bardinlab.bsky.social · 07/10/2025
The PhD work of @bboumard.bsky.social officially out in print! Happy to share this issue with our downstairs colleagues in the BDD @frelab.bsky.social @robinjournot.bsky.social www.cell.com/developmenta...
cell.com
Cell-type-specific nucleotide sharing through gap junctions impacts sensitivity to replication stress in Drosophila
Boumard et al. demonstrate gap-junction-dependent tissue-scale nucleotide sharing, which impacts cellular sensitivity to perturbation of nucleotide homeostasis and replication stress. Drosophila wing ...
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 06/10/2025
Our paper is on the cover of @cp-devcell.bsky.social . Image: embryonic murine salivary-gland explants stained for fate determinants; p63 (cyan) and HES1 (yellow). Thanks to everyone involved. doi.org/10.1016/j.de...
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Reposted by Robin Journot 🔬| 🎾
Bart Deplancke @bartdeplancke.bsky.social · 06/10/2025
🧵1/ Excited to share our new paper introducing a new #singlecell assay: scTF-seq, a high-throughput single-cell approach to explore how transcription factor (TF) dose shapes cell identity and reprogramming outcomes. 🔗 www.nature.com/articles/s41... Big congrats to the entire team @EPFL & @SIAT_China
nature.com
Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq - Nature Genetics
This study introduces single-cell transcription factor (TF) sequencing, a single-cell barcoded and doxycycline-inducible TF overexpression approach that reveals dose-sensitive functional classes of TFs and cellular heterogeneity by mapping TF dose-dependent transcriptomic changes during the reprogramming of mouse embryonic multipotent stromal cells.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 30/09/2025
"Contractile fibroblasts form a transient niche for the branching mammary epithelium." Now out: rdcu.be/eIIKD A great contribution from Jakub Sumbal, showing how stromal cells, and diverse fibroblast subsets, regulate branching morphogenesis. @sumbalovakoledova.bsky.social @frelab.bsky.social
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 08/09/2025
From a FACS-contaminating cell population to a multi-organ project — learn the Behind the Story of our work on conserved signals in epithelial organogenesis 👉 www.sciencedirect.com/science/arti...
sciencedirect.com
Conserved signals control self-organization and symmetry breaking of murine bilayered epithelia during development and regeneration
Organ development relies on molecular cues that guide stem cells to differentiate within precise spatial arrangements. After injury, restoring these p…
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 04/07/2025
Yes! We think this mechanism goes way beyond glands. We're now exploring a broader range of tissues, stay tuned for what's coming next!
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 04/07/2025
Great question! We haven’t inferred or directly measured mechanical forces yet, but in a follow-up project we’re actively investigating the mechanical component of the symmetry-breaking event. Very curious to see how it ties into YAP localization and happy to discuss more!
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Reposted by Robin Journot 🔬| 🎾
Bardin Lab @bardinlab.bsky.social · 13/06/2025
Students (Master's/PhD), do not miss this fantastic Developmental Biology course of @sorbonne-universite.fr and @institutcurie.bsky.social - It is FREE and open to international students, however, room and board + travel are not included. training.institut-curie.org/courses/deve...
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
This work was funded by @institutcurie.bsky.social, ‪‪‪@cnrsbiologie.bsky.social, @psl-univ.bsky.social, @agencerecherche.bsky.social , @frm-officiel.bsky.social, @cerclefser.bsky.social, @fondationarc.bsky.social and @worldwidecancer.bsky.social.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
Full publication in @cp-devcell.bsky.social : authors.elsevier.com/c/1lM285Sx5g...
authors.elsevier.com
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
Despite distinct germ layer origins and divergent adult functions, all 4 glands rely on the same YAP–Notch–p63 circuit to specify fate and pattern. We propose an updated hourglass model where this conserved module acts as a developmental bottleneck in organogenesis.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
In regeneration, we saw the same transitional state: YAP⁺p63⁺HES1⁺. → Developmental programs are reused for repair.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
YAP acts then as a capacitor, counting the number of cell within the tissue before allowing cells to differentiate.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
We found the key: YAP. Initially nuclear in all cells, it becomes cytoplasmic in internal cells—coinciding with symmetry breaking and fate commitment. When we forced YAP activity, organoids didn’t break symmetry—cells stayed in a stem-like state, co-expressing p63 & HES1. Same results in embryos!
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
But why do organoids stay homogeneous until ~13 cells? Theoretical models say symmetry can break in 2 cells. Here, an active mechanism seems to delay fate decisions early on.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
We then asked: what regulates this decision? Notch activation (in vivo or organoids) forced luminal fate. Notch inhibition blocked it. → Notch is a gatekeeper of luminal identity. We further demonstrated that symmetry breaking appeared through lateral inhibition.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
We observed that fate segregation (K5 outside, K8 inside) happened not over time, but when organoids reached 13–21 cells. → This suggests size-dependent symmetry breaking drives fate commitment.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
All 4 glands form 2-layered epithelia: K5⁺ basal cells on the outside, K8⁺ luminal facing the lumen. But where does this structure come from during development? We sorted basal stem cells and grew organoids from all 4 tissues. They reactivated multipotency and gave rise to both cell types in vitro.
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 01/07/2025
🧵Just out ! We reveal how 4 branched epithelia—mammary, lacrimal, salivary & prostate—use a conserved YAP–Notch–p63 circuit to self-organize during development & regeneration. Here’s the story👇 authors.elsevier.com/c/1lM285Sx5g... Work done in @frelab.bsky.social at ‪ ‪ @institutcurie.bsky.social
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The EMBO Journal @embojournal.org · 07/04/2025
Notch1 activation in basal cells triggers a progressive basal-to-luminal cell-fate switch in the mouse mammary gland but also in adult cells of the lacrimal, salivary and prostatic glands Silvia Fre and coworkers @frelab.bsky.social @institutcurie.bsky.social www.embopress.org/doi/full/10....
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Cornelia Schwayer @cschwayer.bsky.social · 15/01/2025
I am very excited to share our most recent work on how ‘Cell heterogeneity and fate bistability drive tissue patterning during intestinal regeneration’ ☺️ Thanks a lot to @silviabarbiero.bsky.social @davidbrueckner.bsky.social @priscaliberali.bsky.social @ehannezo.bsky.social and all the co-authors!
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Hani Goodarzi @genophoria.bsky.social · 05/12/2024
@thejohnnyyu.bsky.social, @therealnima.bsky.social , Kevan Shokat, and I are excited to announce a historic achievement by our team at @vevotherapeutics.bsky.social: tinyurl.com/4rrvap3t
prnewswire.com
Vevo Therapeutics to Release World's Largest Atlas of Single-Cell Transcriptomic Data, Tahoe-100M, to Map How Drugs Impact Patient Cells and Accelerate Discovery of New Drugs
/PRNewswire/ -- Vevo Therapeutics, a biotechnology company using its Mosaic technology and next generation AI to uncover better drugs for more patients,...
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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 19/11/2024
A new platform, a new post! Our #Stemcell #DevBio and #Regeneration story by Silvia Fre's lab at InstitutCurie is on biorXiv. Link: tinyurl.com/2uspcd3u We reveal how 4 epithelia self-organize during embryonic development and regeneration.
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