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Gozde Demirer

@prof-gozde.bsky.social
702 followers 285 following 24 posts

Assistant Prof @Caltech | Nanotechnology, synthetic biology, plant genetic engineering, plant-microbe interactions 🌱 🍅 🧬🧪 | she/her

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Reposted by Gozde Demirer
North American Arabidopsis Steering Committee @naascarabidopsis.bsky.social · 25/08/2026
Announcing the Genome Diversity, Variation & Engineering plenary session invited speakers for #ICAR2027Madison! 🌱Gozde Demirer- CalTech 🌱Dan Koenig- UC Riverside 🌱Xiaofeng Cao- Chinese Academy of Sciences info & updates www.arabidopsiscommunity.org/icar2027 @prof-gozde.bsky.social
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Reposted by Gozde Demirer
Sarah Guiziou @sarah-guiziou.bsky.social · 15/04/2025
🚨 PhD project alert: Engineering a soil bacteria to sense and record soil health. 🦠 🪴 🥼 🧑‍💻 🌍 Fully funded PhD studenship in my group, open for international students. @earlhaminst.bsky.social Please share widely! Deadline: 14th of May www.earlham.ac.uk/studentship/...
earlham.ac.uk
Sentinel bacteria: engineering a soil bacteria to sense and record soil health
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Gozde Demirer @prof-gozde.bsky.social · 11/07/2026
This is so cool! Thank you for making this @kiranrajrki.bsky.social
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Reposted by Gozde Demirer
Nature Biotechnology @natbiotech.nature.com · 19/06/2026
Optimized R2 retroelement complexes for DNA insertion into plant genomes - @prof-gozde.bsky.social @caltech.edu www.nature.com/articles/s41...
nature.com
Optimized R2 retroelement complexes for DNA insertion into plant genomes - Nature Biotechnology
R2 retrotransposons are used to integrate DNA into plant and crop 25S ribosomal DNA sites.
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
The R2 system is designed to slot into your existing pipeline: swap in the R2 protein + payload plasmid in place of your Cas9/donor and give it a try for targeted, multi-kb insertion at the rDNA safe harbor in plants. 🌱 If you are interested in testing the system, feel free to reach out.📩
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
We inserted a 5kb, 3-enzyme RUBY pathway: visible red pigment at ~1 copy/genome. Since efficiency didn't drop from 2 to 5kb, there's likely room for larger DNA. It worked across 3 species: Arabidopsis, N. benthamiana, and tomato. Bonus: inserted payloads stayed unmethylated and expression persisted.
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
1 integrated copy per genome on average, with> 90% being full-length insertions. At the same rDNA site, R2 is 30× more efficient than Cas9 HDR. >96% of recovered insertions mapped to 25S rDNA sites. 5′ junctions were seamless “anneal-type” (97.7%), confirmed by Nanopore long-read mapping.
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
Getting it to work in plants took geminiviral replicons and introns to boost R2 levels, a minimal-homology RNA payload, optimized 5′ and 3′ UTRs, and an HDV ribozyme for a clean 3′ end. The payoff: precise mCherry insertion went from ~0.5% of cells in the first screen to ~24% of transformed cells.
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
The mechanism is double-strand break-free. The R2 ribonucleoprotein nicks one strand, primes off it, and reverse-transcribes the RNA payload directly into the genome, sidestepping the double-strand breaks (and the deletions/rearrangements they can cause) that Cas9-based insertion depends on.
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
25S rDNA is a natural genomic "safe harbor": many copies, highly transcribed, no essential genes nearby. As inserted genes sit away from regulatory elements, they get predictable, high expression. 👀Spoiler: we're making the R2 insertion site reprogrammable to target any locus. Stay tuned! 🔮
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
Our approach: the R2 protein from the zebra finch (Taeniopygia guttata) is a natural retroelement that inserts itself into the 28S/25S ribosomal DNA using its own RNA as the template, via target-primed reverse transcription. We adapted it to do the same in plants but to insert our cargoes.
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
The problem: most plant engineering relies on Agrobacterium, which drops DNA in at random. That means variable expression, silencing, and generations of screening to find a good line. Targeted methods like Cas9 HDR and prime editing help but are limited in efficiency or payload size.🧬
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Gozde Demirer @prof-gozde.bsky.social · 19/06/2026
Targeted DNA insertion remains a major bottleneck in plant engineering. We developed an avian R2 retrotransposon as an efficient plant genome editor to precisely insert large DNA payloads at targeted sites: www.nature.com/articles/s41... Congratulations to @kimuchenje03.bsky.social and the team! 🧵👇
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Gozde Demirer @prof-gozde.bsky.social · 16/05/2026
Caltech Chemical Engineering is recruiting a long-term lecturer! Reach out if you’re interested and have any questions. applications.caltech.edu/job/chemeng
applications.caltech.edu
Long-Term Lecturer in Chemical Engineering | Caltech Applications
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Natanella Illouz-Eliaz @natanellae.bsky.social · 12/05/2026
Excited to share that I will be opening my lab this fall at the @weizmanninstitute.bsky.social in the Department of Plant & Environmental Sciences! I am looking for postdocs interested in plant biology, stress biology, and single-cell technologies. #PlantBiology #SingleCell #Epigenomics #Postdoc
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Evan Groover @evangroover.bsky.social · 31/03/2026
Excited to see the major chapter of my PhD in print! We present a massively-parallel approach for screening CRISPR edits in plants to speed up agricultural test cycles + benchmark genomic LMs. Thanks to my fantastic co-authors and advisors. Link below!
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Gozde Demirer @prof-gozde.bsky.social · 18/03/2026
Hi, no, it is not specific to those topics but they are welcome.
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Gozde Demirer @prof-gozde.bsky.social · 17/03/2026
We're hiring a postdoctoral researcher in plant genome engineering with expertise in synthetic biology, gene editing, and omics, with an interest in developing next-generation technologies for plants.🪴🧬 Available immediately. If interested, send your CV! Feel free to share with your networks.
demirerlab.com
Home | Demirer Lab
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Reposted by Gozde Demirer
Tatsuya Nobori @tatsuyanobori.bsky.social · 15/01/2026
ECRs in plant–microbe interactions: Join us in Norwich for an intensive, interactive 1.5-week Summer Conference at TSL (20–31 July). Lots of opportunities for networking and deep discussions. (We also plan to offer hands-on practical sessions using our technologies 🔬🧬)
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Reposted by Gozde Demirer
Brady Lab @bradylabs.bsky.social · 06/11/2025
Interested in learning about the latest and greatest research in how plants respond to excess salt, too little or too much water? Come to the GRC in Salt and Water Stress in Switzerland. Lots of opportunities for all career levels. www.grc.org/salt-and-wat.... Please repost!
grc.org
2026 Salt and Water Stress in Plants Conference GRC
The 2026 Gordon Research Conference on Salt and Water Stress in Plants will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.
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Trevor Nolan @trevormnolan.bsky.social · 07/11/2025
Our new review in Science @science.org is out with @jennyrussinova.bsky.social and @nvukas.bsky.social. We discuss how precise modulation of brassinosteroid signaling can enable more targeted, predictable improvements in plant growth and resilience. science.org/doi/10.1126/science.adu9798
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Trevor Nolan @trevormnolan.bsky.social · 07/11/2025
It was great to talk science and show @billgates.bsky.social around our lab at @caltech.edu . Nolan and Meyerowitz lab postdoc Donovan Phua's regenerating callus is featured 🌱 www.instagram.com/reel/DQo2pen...
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Nature Reviews Molecular Cell Biology @natrevmcb.nature.com · 25/09/2025
Our cover indicates that while we celebrate the past we're looking ahead to the future - with the help of a Viewpoint! 13 researchers offer a glimpse of what their research field might look like in 2050 🔮 👉 go.nature.com/4neNdYU
go.nature.com
Visions of the future of molecular cell biology - Nature Reviews Molecular Cell Biology
To celebrate the journal’s 25th anniversary, we asked 13 researchers to offer a glimpse of what their research field might look like in 2050. They consider how technological breakthroughs — for example, artificial intelligence-powered virtual cells — could transform our understanding of how molecules, organelles and cells behave in different contexts, revolutionize therapies and enable the design of resilient crops.
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Gozde Demirer @prof-gozde.bsky.social · 26/08/2025
4️⃣ A 3D-Printed Capillary Tube Holder for High-Throughput Chemotaxis Assays: We developed a 3D-printed capillary tube holder that enables 384 simultaneous chemotaxis assays, making it possible to rapidly profile bacterial responses to root exudates and other chemoeffectors. tinyurl.com/3nw9haes
tinyurl.com
A 3D-Printed Capillary Tube Holder for High-Throughput Chemotaxis Assays
Bacterial chemotaxis is an important behavior to study to understand spatial segregation of species in mixed communities and the assembly of host microbiomes. This is particularly relevant in the rhiz...
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Gozde Demirer @prof-gozde.bsky.social · 26/08/2025
3️⃣ Vacuum and sonication treatment (VAST) enables efficient transient gene expression in various monocot and eudicot plant seedlings: A transient expression method that dramatically improves gene expression efficiency and uniformity across several monocot and eudicot seedlings. tinyurl.com/38p2ud6w
doi.org
Vacuum and sonication treatment enables efficient transient gene expression in various monocot and eudicot plant seedlings
Transient gene expression in intact plants is essential for rapidly addressing biological questions, where the current toolkit can be improved for higher efficiencies and broader plant species range. ...
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Gozde Demirer @prof-gozde.bsky.social · 26/08/2025
2️⃣ Optimized R2 Retroelement Complexes Enable Precise and Efficient DNA Insertion into Plant Genomes: We engineered R2 protein to insert multi-kb DNA precisely into a safe harbor site in plants achieving 24% efficiency and 1 copy insertion per genome. Compact, precise, powerful! tinyurl.com/5n73za6h
doi.org
Optimized R2 Retroelement Complexes Enable Precise and Efficient DNA Insertion into Plant Genomes
Precise, targeted insertion of multi-kilobase DNA sequences is critical for studying gene function, ensuring robust transgene expression, and stacking traits in crops, but remains difficult to achieve...
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Gozde Demirer @prof-gozde.bsky.social · 26/08/2025
1️⃣ Extracellular contractile injection systems for high efficiency protein delivery to plants: We adapted bacterial PVC nanomachines to deliver proteins (Cre recombinase) to plants with 40% efficiency. DNA-free genome engineering with potential for cell-type specific targeting. tinyurl.com/mrx4d878
tinyurl.com
Extracellular contractile injection systems for high efficiency protein delivery to plants
Efficient delivery of functional proteins into plant cells remains a major barrier in plant biotechnology. Extracellular contractile injection systems (eCISs) are phage tail-like nanomachines evolved ...
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Gozde Demirer @prof-gozde.bsky.social · 26/08/2025
Big news from the Demirer lab: first 4 research papers are preprinted! Check them out, feedback welcomed: eCIS for plant protein delivery: tinyurl.com/mrx4d878 R2 for targeted insertion: tinyurl.com/5n73za6h Transient expression: tinyurl.com/38p2ud6w Chemotaxis assay: tinyurl.com/3nw9haes More ⬇️
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Gozde Demirer @prof-gozde.bsky.social · 26/07/2025
Excited to be at #PlantBiology2025 🌱 this week and deeply honored to receive the Jane Silverthorne Early Career Award this year! aspb.org/awards-fundi... Let me know if you’re around and want to catch up at the conference! #ASPB #PlantBiology2025
aspb.org
Early Career Award | American Society of Plant Biologists
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Gozde Demirer @prof-gozde.bsky.social · 18/07/2025
Excited to be speaking at Synthetic Biology Australasia 2025 in Wellington, New Zealand, 19–21 November! I'll be sharing my group's recent advances on plant synthetic biology and genome engineering 🌱🧬 Join us: 🔗 synbioaustralasia.org/upcoming-conference #SBA2025 #SyntheticBiology #ScienceInAction
synbioaustralasia.org
SBA 2025 conference
SBA 2025 conference Synthetic Biology Australasia New Zealand synbio conference
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Reposted by Gozde Demirer
Jean-Michel Ané @jeanmichelane.bsky.social · 04/03/2025
Our latest article on biological nitrogen fixation on the aerial roots of sorghum has been published in @plosbiology.org. We thank the @energygov.bsky.social for supporting this exciting project. journals.plos.org/plosbiology/...
Sorghum aerial roots producing mucilage and hosting bacteria that fix nitrogen from the air
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Gozde Demirer @prof-gozde.bsky.social · 11/12/2024
Join us at the 2025 International Conference on Biomolecular Engineering (ICBE) for cutting-edge discussions and an incredible speaker lineup! 🌟 Last day to register (with hotel room) is this Friday, Dec 13! #ICBE2025 🔗Register here: www.aiche.org/sbe/conferen...
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Gozde Demirer @prof-gozde.bsky.social · 26/11/2024
🌱 Demirer Lab 🌱 @caltech.edu is looking for passionate graduate students to join us! Explore our exciting research for a sustainable future: demirerlab.com 🌏 🌱 🦠 📢 Apply here to CCE or BBE: t.co/cIv8Hbfy0Z and mention your interest in our lab in your statement. 🗓️ Application deadline: Dec 1
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Gozde Demirer @prof-gozde.bsky.social · 26/11/2024
We’re looking for talented, driven graduate students to join our growing Chemical Engineering Ph.D. program at @caltech.edu! ⏰ Applications are due December 1, 2024. ➡️ Apply Here: t.co/cIv8Hbfy0Z
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Marc Somssich @somssich.bsky.social · 11/11/2024
Hello new Plant Scientists, 2 Things: 👉 To connect to 150 Plant Science Accounts and 3 relevent feeds, here's a #StarterPack for you: go.bsky.app/8Zfmwf7 👉 To get your #PlantScience posts into the Plant Science Research Feed (here: bsky.app/profile/did:...), always use the Hashtag #PlantScience
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Gozde Demirer @prof-gozde.bsky.social · 05/11/2023
We made it to Orlando for the #AIChE2023! This is Mark’s @mlegendr.bsky.social debut to the AIChE community!! Check out his poster (#111) on Monday 3:30 pm discussing our recent work on genetically encoded delivery vehicles for plants 🌱
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