An alkyne-tagged ethanolamine analog (AlkEA) enables bioorthogonal, metabolic labeling of endogenous phosphatidylethanolamine (PE) and its protein modifications. AlkEA is incorporated into PE via the...

Nishimura et al. develop a platform named cell surface liposome binding (CLiB), which allows the high-throughput monitoring of protein–lipid interactions and can be used to design lipid-binding probes...

Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.

Lipid-binding domains, originally isolated from natural proteins, are useful tools essential for analyzing membrane lipids in cells, and their applications are varied. Yet, there is no general strateg...