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Lechtenberg Lab @lechtenberglab.bsky.social · 29/07/2025

We are still accepting abstracts for selected short talks and posters. The abstract deadline has been extended to this Friday, 1st Aug. More information and registration here: www.lorneubiquitin.org .

lorneubiquitin.org

The Australian Ubiquitin Summit 2025

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Lechtenberg Lab @lechtenberglab.bsky.social · 07/07/2025

📣📣 Lorne Ubiquitin – The Australian Ubiquitin Summit 📣📣 20-23 Nov 2025 in Lorne, Australia Join us this Nov for the first ubiquitin meeting in Australia. Submit your abstract by 18 July 2025 for oral and poster presentations: www.lorneubiquitin.org. Please share and repost.

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Lechtenberg Lab @lechtenberglab.bsky.social · 10/04/2025

Excited to share our latest work, now out in @lsajournal.org. we show that the E3 ligase HOIL-1 can ubiquitinate many different saccharides and describe a chemoenzymatic platform to generate ubiquitinated sugars as tools for studying non-proteinaceous ubiquitination.

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Lechtenberg Lab @lechtenberglab.bsky.social · 03/12/2024

We hope that our protocol will enable us and others to generate various ubiquitinated saccharides as tools to further develop the non-proteinaceous ubiquitin field. A plasmid for bacterial expression of the HOIL-1 variant will be available at @addgene.bsky.social soon.

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Lechtenberg Lab @lechtenberglab.bsky.social · 03/12/2024

We utilise HOIL's broad saccharide substrate specificity to develop a simple protocol for the chemoenzymatic generation of preparative amounts of ubiquitinated saccharides. Our approach is vastly simplified by our engineered constitutively active HOIL-1 variant.

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Lechtenberg Lab @lechtenberglab.bsky.social · 03/12/2024

We find that HOIL-1 efficiently ubiquitinates hydroxyl groups in Ser and a wide variety of mono- and disaccharides. We show that HOIL-1's His510 residues is critical for this activity and that mutation of His510 to Ala switches HOIL-1 activity from hydroxyl groups to targeting amines.

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Lechtenberg Lab @lechtenberglab.bsky.social · 03/12/2024

HOIL-1 is an E3 ubiquitin ligase that can ubiquitinate non-proteinaceous substrates such as glycogen and maltose as first shown in work by @iankelsall.bsky.social. In our work, we systematically compare HOIL-1's in vitro activity against a number of amino acids (Lys, Ser, Thr) and saccharides.

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Reposted by Lechtenberg Lab

Rune Busk Damgaard @rbdamgaard.bsky.social · 15/11/2024

Check out this list of ubiquitin and Ubl profiles to follow. Repost if you want me to add you to it. Let’s create a good community here ☁️ go.bsky.app/3VPQofn

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