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Lachlan Coin

@lachlanjmc.bsky.social
324 followers 632 following 24 posts

Computational biologist working on infectious disease

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Reposted by Lachlan Coin
Nucleic Acids Research @narjournal.bsky.social · 22/09/2026
✨ #NARGenomicsandBioinformatics✨ 🎯 What if you could enrich #RNAtranscripts by up to 60-fold without losing relative abundance information? Researchers @lachlanjmc.bsky.social combine RNA-guided CRISPR-Cas9 with long-read sequencing for customizable, targeted transcript enrichment. 🧬
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Lachlan Coin @lachlanjmc.bsky.social · 22/09/2026
New paper from the lab using CRISPRi and native RNA sequencing to study bacterial RNA modifications dx.doi.org/10.1002/mbo3... .. great work from @mirandapitt.bsky.social
dx.doi.org
Interrogating the <i>Escherichia coli</i> Epitranscriptome Via CRISPR Interference and Nanopore Native RNA Sequencing
Epitranscriptomics is understudied in bacteria but has the ability to modulate antimicrobial resistance, virulence and pathogenicity. Pitt et al. implement two cutting-edge techniques: CRISPR interfe...
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Lachlan Coin @lachlanjmc.bsky.social · 20/09/2026
Our article on customisable host and viral transcript enrichment using CRISPR-Cas9 and long-read sequencing is out at NAR genomics and bioinformatics: lnkd.in/gJpAjz2G . We show that we can use CRISPR-Cas9 to achieve ~60 fold enrichment of targets from a cDNA library. doi.org/10.1093/narg...
doi.org
Customizable host and viral transcript enrichment using CRISPR–Cas9 long-read sequencing for characterization of low-to-moderate abundance isoforms
Abstract. One of the main challenges of whole transcriptome sequencing is the difficulty in detecting and quantifying low-to-moderate abundance transcripts
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Reposted by Lachlan Coin
Jovana Maksimovic @jovmaksimovic.bsky.social · 26/11/2025
media.tenor.com
a woman in a black jacket is giving a peace sign
ALT: a woman in a black jacket is giving a peace sign
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Reposted by Lachlan Coin
Nikeisha Caruana @nikeishacaruana.bsky.social · 24/11/2025
Global equity of data is only possible when countries can have ownership and generate their own data. Data equity and access to infrastructure is so important. A great presentation by @ebi.embl.org Interim Director Dr. Joanna McEntyre @abacbs.bsky.social #ABACBS2025
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Reposted by Lachlan Coin
Michael Hall @mbhall88.bsky.social · 20/11/2025
🚨 Update to nohuman 🚨 While testing against the standard Kraken DB, I noticed Kraken was detecting far more human reads than nohuman. I realised Kraken masks low-complexity regions by default during DB construction and that setting had been left on in nohuman, leading to missing human reads.
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Lachlan Coin @lachlanjmc.bsky.social · 07/11/2025
Huge thanks to Chenxi Zhou for helping scale this approach to human sized genomes!
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Albert Vilella, PhD. @albertvilella.bsky.social · 04/09/2025
A paper from @lachlanjmc.bsky.social Lachlan Coin, not active here for the past month, on Using synthetic RNA to benchmark poly(A) length inference from direct RNA sequencing academic.oup.com/gigascience/...
academic.oup.com
Using synthetic RNA to benchmark poly(A) length inference from direct RNA sequencing
Abstract. Polyadenylation is a dynamic process that is important in cellular physiology, which has implications in messenger RNA decay rates, translation e
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Dr. Damien P. Williams, dread portent down from a mountain cave @wolven.blacksky.app · 22/08/2025
Louisiana has to build three new natural gas power plants to accommodate the "AI" data center that Meta just crammed through because said center will use *Three Times* as much electricity (and, thus, attendant resources) as the *Entire City Of New Orleans*, every year. Y'all this isn't sustainable.
kplctv.com
La. regulators approve Entergy power plants for Meta’s AI data center
Louisiana Public Service Commissioners voted four to one to approve Entergy’s three new gas plants to power Meta’s largest-ever data center.
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Reposted by Lachlan Coin
Reese Richardson @reeserichardson.bsky.social · 04/08/2025
Today, our article "The entities enabling scientific fraud at scale are large, resilient, and growing rapidly" is finally published in PNAS. I hope that it proves to be a wake-up-call for the whole scientific community. reeserichardson.blog/2025/08/04/a...
reeserichardson.blog
A do-or-die moment for the scientific enterprise
Reflecting on our paper “The entities enabling scientific fraud at scale are large, resilient, and growing rapidly”
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Lachlan Coin @lachlanjmc.bsky.social · 24/07/2025
Our latest preprint using long read nanopore sequencing to study Ferret transcriptomic response to infection and interferon stimulation, lead by Rubaiyea Farrukee and Jessie Chang www.biorxiv.org/content/10.1...
biorxiv.org
Characterizing the transcriptomic response to interferon and infection in European Domestic Ferret respiratory tissues using long-read RNA sequencing
The European domestic ferret (Mustela putorius furo) is considered the gold-standard small animal model for studying human and avian influenza virus infections. However, experimental characterization ...
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Lachlan Coin @lachlanjmc.bsky.social · 26/06/2025
New paper from our lab in collaboration with Blaskovich lab led by former Phd student Anggia Prasetyoputri using in vitro selection to identify drug resistance conferring murations in S. aureus academic.oup.com/jacamr/artic...
academic.oup.com
Characterisation of in vitro resistance selection against second-/last-line antibiotics in methicillin-resistant Staphylococcus aureus ATCC 43300 strain
AbstractBackground and objectives. The increasing occurrence of MRSA clinical isolates harbouring reduced susceptibility to mainstay antibiotics has escala
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Lachlan Coin @lachlanjmc.bsky.social · 09/05/2025
New preprint from @aleixcanalda.bsky.social on structural variation in TB and its role in evolution and drug resistance www.biorxiv.org/content/10.1...
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Lachlan Coin @lachlanjmc.bsky.social · 15/04/2025
New preprint from the lab, describing an approach to enrichment of transcript isoforms for nanopore cDNA sequencing, using CRISPR-Cas9 www.biorxiv.org/content/10.1...
biorxiv.org
Customizable host and viral transcript enrichment using CRISPR-Cas9 long-read sequencing for isoform discovery and validation
Long-read RNA sequencing has been broadly utilized to examine the diversity of transcriptomes, understand differential expression and discover novel transcript isoforms. One of the major limitations o...
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bioRxiv Genomics @biorxiv-genomic.bsky.social · 15/04/2025
Customizable host and viral transcript enrichment using CRISPR-Cas9 long-read sequencing for isoform discovery and validation www.biorxiv.org/content/10.1101/202…
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Ewan Birney @ewanbirney.bsky.social · 19/12/2024
I want to provide an overview and perspective of rare disease genomic testing, which is a real success story of science impacting health - a science which goes back over 100 years and yet is still striding forward and much more to come.
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Lachlan Coin @lachlanjmc.bsky.social · 15/12/2024
Latest preprint from the lab: we asked whether native RNA sequencing (using nanopore sequencing) might be a successor to traditional cDNA sequencing for biomarker discovery in blood.
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Robert Aboukhalil @robert.bio · 04/12/2024
“We don’t value software, data, and methods in the same way we value papers, even though those resources empower millions of scientists” 💯 www.statnews.com/sponsor/2024...
statnews.com
New report highlights the scientific impact of open source software
Two of the scientists who won this year’s Nobel Prize for cracking the code of proteins’ intricate structures relied, in part, on a series of computing
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bioRxiv Bioinfo @biorxiv-bioinfo.bsky.social · 02/12/2024
Genome size estimation from long read overlaps www.biorxiv.org/content/10.1101/202…
biorxiv.org
Genome size estimation from long read overlaps https://www.biorxiv.org/content/10.1101/2024.11.27.625777v1
Summary: Accurate genome size estimation is an important component of genomic analyses, though exist
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Michael Hall @mbhall88.bsky.social · 03/12/2024
🌟 Excited to share my latest preprint with @lachlanjmc.bsky.social on @biorxivpreprint.bsky.social: "Genome size estimation from long read overlaps”! 🚀 Check it out here: doi.org/10.1101/2024... And find the code here: github.com/mbhall88/lrge 🧵👇
doi.org
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Lachlan Coin @lachlanjmc.bsky.social · 18/11/2024
Was inspiring to attend the TBunion conference in Bali last week and learn about the great TB research happening all over the world. Probably one of the most international conferences I have been to (in terms of number of countries represented)
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