iiinova — where your digital self keeps evolving

kuba sędziński @kuba-sedzinski.bsky.social · 17/08/2026

Pleasure is ours! Congratulations on such an amazing work!

Reposted by kuba sędziński

Journal of Cell Biology @jcb.org · 17/08/2026

Spotlight: @babliadhikary.bsky.social et al. (rupress.org/jcb/article/...) describe the role of PAK4 in vertex remodeling, thereby maintaining epithelial integrity and barrier function. @raghavan-thiaga.bsky.social and @kuba-sedzinski.bsky.social discuss the study: rupress.org/jcb/article/...

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Reposted by kuba sędziński

Rockefeller University Press @rupress.org · 17/08/2026

Spotlight @jcb.org: @babliadhikary.bsky.social et al. (rupress.org/jcb/article/...) describe the role of PAK4 in vertex remodeling, thereby maintaining epithelial integrity & barrier function. @raghavan-thiaga.bsky.social and @kuba-sedzinski.bsky.social discuss the study: rupress.org/jcb/article/...

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kuba sędziński @kuba-sedzinski.bsky.social · 14/08/2026

We managed! Just one ikea drawer died 🫠

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kuba sędziński @kuba-sedzinski.bsky.social · 11/08/2026

Indeed! Quite some years! Hope to catch up with you💪

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kuba sędziński @kuba-sedzinski.bsky.social · 11/08/2026

Thanks! Yes, it is going to be a long day!

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kuba sędziński @kuba-sedzinski.bsky.social · 11/08/2026

🐸❤️! Thank you! Hope we find our way😎

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kuba sędziński @kuba-sedzinski.bsky.social · 11/08/2026

We have it all! Got all logistic tools 😎

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kuba sędziński @kuba-sedzinski.bsky.social · 11/08/2026

Lab packed. Home packed. Somehow, everything fits in a truck. 📦🧪🚚 After 9.5 amazing years in Copenhagen, it’s time for the next adventure. Germany, here we come! 🇩🇪

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kuba sędziński @kuba-sedzinski.bsky.social · 11/08/2026

Lab packed. Home packed. Somehow, everything fits in a truck. 📦🧪🚚 After 9 amazing years in Copenhagen, it’s time for the next adventure. Germany, here we come! 🇩🇪

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kuba sędziński @kuba-sedzinski.bsky.social · 09/07/2026

Beautiful overview on basal body patterning by @raghavan-thiaga.bsky.social and of course please check his latest work on dynamics of basal body patterning in 🐸 multiciliated cells!

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Reposted by kuba sędziński

epithelial mechanics fan club @epimechfc.bsky.social · 30/06/2026

Thiagarajan, R., Barooji, Y. F., Bendix, P. M., Inamdar, M. M., & Sedzinski, J. (2026). Actin cross-linking organizes basal body patterning through anomalous diffusion transitions. arXiv preprint arXiv:2605.15912. #EpithelialMechanics www.biorxiv.org/content/10.6...

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kuba sędziński @kuba-sedzinski.bsky.social · 05/06/2026

We always deliver our thesis by hand as we do not trust the Danish post:) Seriously, we are just happy to have you and your team as friends ❤️ @MariTolonen, 🙏

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Reposted by kuba sędziński

John Wallingford @jbwallingford.bsky.social · 26/05/2026

Only FIVE DAYS LEFT to register for the 2026 Santa Cruz Dev Biol meeting! Great speakers! Tons of talks selected from abstracts! Workshop with Scott Fraser! Workshop with Rashmi Priya! Redwoods trees! 🧬🐸🐟🪰🐭🪱🍃🔥🔬. @devbiol.bsky.social @socdevbio.bsky.social scdb2026.sites.ucsc.edu

scdb2026.sites.ucsc.edu

Santa Cruz Developmental Biology Meeting

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kuba sędziński @kuba-sedzinski.bsky.social · 26/05/2026

Great new tool to infer forces from static and dynamic images💪😎

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kuba sędziński @kuba-sedzinski.bsky.social · 20/05/2026

Thank you for shout out! Much appreciated!

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kuba sędziński @kuba-sedzinski.bsky.social · 20/05/2026

In fact, in a-actinin 1 KD, there are no cages around BBs, which itself suggests that it is the apical actin network that gets reorganised through x-linking, thereby creating the pockets in which BBs get stuck.

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kuba sędziński @kuba-sedzinski.bsky.social · 20/05/2026

This is difficult to say, but indeed it is more likely that the actin network acts as a surrounding viscoelastic meshwork that constrains basal body motion through steric hindrance, frictional coupling, and transient physical interactions that ultimately generate cages around BBs.

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kuba sędziński @kuba-sedzinski.bsky.social · 20/05/2026

We propose that the (hyper-)regularity emerges dynamically from actin-mediated constrained basal body dynamics. Progressive confinement and steric interactions suppress large density fluctuations and promote homogeneous basal body spacing during apical expansion.

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kuba sędziński @kuba-sedzinski.bsky.social · 20/05/2026

Yes, BBs do bring actin regulators with them that could help in making the cushion.

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kuba sędziński @kuba-sedzinski.bsky.social · 20/05/2026

In our system, fate and shape seem to go hand in hand.

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kuba sędziński @kuba-sedzinski.bsky.social · 20/05/2026

Interesting! Let us check! Cheers!

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Actin network undergoes cross-linking which changes the dynamics of BB from diffusive to subdiffusive. So, actin largely fine-tunes the movement of BBs. But also it is important for their initial insertion at the apical domain.

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Thanks to @Younes Farhangi Barooji, @Poul Martin Bendix, @Mandar Inamdar, and most importantly to @raghavan-thiaga.bsky.social, who drove this entire project most professionally!

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

So in the end, actin is the hero 🦸. It is actively sculpting BB dynamics, progressively guiding the system from disorder to order. More broadly, the cytoskeleton can actively tune organelle dynamics to build order. Hope you enjoy the preprint! 🚀 (17/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Even more striking: With alpha-actinin perturbation, BBs clustered to one side 😮 Why? Because actin becomes heterogeneous - and BBs follow this heterogeneity. They accumulate where actin is enriched 😎. (16/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

So we broke this. When we disrupt actin crosslinking (alpha-actinin), everything falls apart: ❌ kinematics ❌ diffusion transitions ❌ uncaging → BB organization collapses. 😇 Confirming that actin crosslinking tunes BB dynamics toward order 🍪 (15/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

So is actin really driving this? Yes 🎉 - and here’s the key: The moment actin starts organizing into a crosslinked meshwork is exactly when BB dynamics change 🙌. Same transition at the same area expansion range 🥂 . (14/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

When we looked closely, it was all there. High-resolution imaging confirmed that actin gradually organizes into a crosslinked meshwork around BBs. During expansion, it transitions from disordered → structured, forming “cushions” that keep BBs apart 🪢🪢🪢 🦾 (13/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Of course, BBs don’t literally have springs. But springs mean elasticity… and cells are viscoelastic. So naturally, we started thinking about the cytoskeleton - previous works hint strongly at actin related regulation 🙏 (12/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

At this point, we needed theoretical modelling 💡. With @ Mandar Inamdar, we built a particle trap model and it suggested something unexpected: BBs behave as if they are surrounded by springs, keeping them separated at all times ⚙️ 🦾 (11/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

2️⃣ Zooming into short times: BBs get caged and uncaged. This uncaging takes longer as the apical area grows ⏳ 🔥 (10/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

That’s where things got interesting with two key findings: 1️⃣ BB motion shifts from diffusive → sub-diffusive during expansion (9/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

The title already gives it away 😏 - actin. But how does actin do this? We went high-speed: fast imaging of BBs to capture fine-scale motion (thanks to Younes Farhangi Barooji + @Bendixlab 🙏). Then MSDs, fine-scale dynamics… everything 🤓 (8/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

At this point, it almost felt neat: BBs are being advected by the growing surface 🙌. Not quite. Plot twist ⚡: only ~50% follow the expanding edge. The rest ignore the periphery, do their own movements… So then, what is really positioning them ? (7/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Then we asked: where do new BBs actually show up? Turns out, they mostly appear near the newly expanded edge. So there is a strong coupling between growth and BB addition. And strikingly, all kinematic features showed this coupling with expansion 🔥 (6/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Then something striking popped up: Even though bbs were continuously added, BB density stays constant during expansion. Even cooler: BBs can exit the apical plane, wander into the cytoplasm, and re-enter later. Yes… they try again until accepted 🤯 (mechanism unknown 🤔!) (5/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

We watched carefully 🔬: as the apical surface expanded, BBs were added, moved around, and rearranged. We quantified BB number, spatial organization (NND, Voronoi), and finally went deep into kinematics: trajectories, tortuosity, distances, drift, MSD… the full package 😎 (4/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Basal bodies (BBs) are what eventually become motile cilia. So, if they don't get positioned correctly on the apical surface, the whole tissue-level function can go wrong → ciliopathies. We know actin is involved… but how does it actually control this process? 🤔 (3/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

Actin cross-linking organizes basal body patterning through anomalous diffusion transitions (2/17) www.biorxiv.org/content/10.6...

biorxiv.org

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kuba sędziński @kuba-sedzinski.bsky.social · 19/05/2026

💥 🚀 New preprint! 🎉🥳 How do hundreds of organelles organize themselves into near-perfect patterns inside a cell, without a blueprint? We dive deep into how basal bodies (BBs) self-organize in MCCs - and how actin actively tunes their dynamics into order 🍪 🧵👇 (1/17)

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kuba sędziński @kuba-sedzinski.bsky.social · 16/05/2026

Wow! Spectacular! Lots of cool things in these 🐸

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