Sign in

Aaron Kuan

@kuanawanda.bsky.social
140 followers 99 following 8 posts

Assistant prof @yaleneuro.bsky.social: multiscale connectomics with EM and Xray; decision-making; brain-wide cortical networks.

PostsRepliesMedia
Aaron Kuan @kuanawanda.bsky.social · 23/06/2026
Congrats!!
010
Reposted by Aaron Kuan
Nature Methods @natmethods.nature.com · 08/12/2025
Our Method of the Year 2025 is...drumroll please...EM-based connectomics!! Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬 www.nature.com/articles/s41...
nature.com
Method of the Year 2025: electron microscopy-based connectomics - Nature Methods
A large network of interconnected neurons serves as the basis of brain function and of behavior. Methodological advances have enabled the reconstruction of large-scale and even whole-brain connectomes...
15321
Reposted by Aaron Kuan
Moritz Helmstaedter @mhlab.bsky.social · 08/12/2025
Connectomics is Method of the Year 2025!! @natmethods.nature.com just released the news nature.com/articles/s41... In my perspective piece, I afford some predictions into the future and compare how we are doing vs. genomics www.nature.com/articles/s41...
12816
Reposted by Aaron Kuan
Andreas Schaefer @andreas-t-schaefer.bsky.social · 27/11/2025
X-rays can resolve ultrastructure - milestone for ultrastructure imaging w/o cutting. Thanks to an amazing team - @carlesbosch.bsky.social @adrianawanner.bsky.social Ana Diaz and collaborators @crick.ac.uk @psich.bsky.social and @esrf.fr and funders @wellcometrust.bsky.social @ukri.org
292
Reposted by Aaron Kuan
Adrian Wanner @adrianawanner.bsky.social · 27/11/2025
Happy to share the first paper from my journey at @psich.bsky.social towards X-ray connectomics, now out in @natmethods.nature.com: www.nature.com/articles/s41...
14015
Reposted by Aaron Kuan
Carles Bosch @carlesbosch.bsky.social · 27/11/2025
#paper! X-rays can resolve #ultrastructure in tissues non-destructively. www.nature.com/articles/s41... Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.
nature.com
Nondestructive X-ray tomography of brain tissue ultrastructure - Nature Methods
Optimizations of X-ray nanotomography including the choice of resin allows high-resolution imaging of mouse brain tissue, approaching the resolution of volume electron microscopy. Since it does not re...
1107
Aaron Kuan @kuanawanda.bsky.social · 27/11/2025
This work was made possible by amazing collaborators including @biedererlab.bsky.social, Tony Koleske, James Rothman, @pdc-lab.bsky.social, Gary Desir, Xinran Liu, and funding from NIH, @wellcometrust.bsky.social, @kavliatyale.bsky.social, Brain Research Foundation, Shank2 Foundation. (5/5)
000
Aaron Kuan @kuanawanda.bsky.social · 27/11/2025
Indeed, we show that "pan-ExM" enables tracing of the thinnest neuronal wires (spine necks and thin axons) and can be scaled up to produce connectomes. Combining with antibody labeling enables “molecular connectomics”, which can unite cellular/molecular and systems understandings of the brain. (4/5)
120
Aaron Kuan @kuanawanda.bsky.social · 27/11/2025
Because this protocol retains proteins in the gel, it is natively compatible with antibody labeling, allowing localization of synaptic proteins, cell-type and subcellular markers with ultrastructural resolution, effectively a sort of “multicolor electron microscopy”. (3/5)
110
Aaron Kuan @kuanawanda.bsky.social · 27/11/2025
We developed a "pan-expansion" protocol that works well in brain tissue, using a ~20x iterative expansion compatible with pan-protein and lipid stains to reveal dense, nanoscale ultrastructure of neuronal circuits (~20 nm effective resolution) with a confocal microscope. (2/5)
110
Aaron Kuan @kuanawanda.bsky.social · 27/11/2025
Our new paper showcasing molecular connectomics with pan-expansion microscopy is out in @natbiotech.nature.com! www.nature.com/articles/s41... This wonderful collaboration with @bewersdorflab.bsky.social was led by Ons M'Saad (now founder/CEO of Panluminate) and @allisonphysics.bsky.social. (1/5)
16528
Reposted by Aaron Kuan
Carles Bosch @carlesbosch.bsky.social · 23/09/2025
#Nanoimaging symposium @crick.ac.uk! At #CrickXrays25 we'll tackle: 💫synchrotron upgrades 📈current limits in throughput, volume, resolution 🧪sample preparation for X-ray PC imaging 🧠♥️applications in neuroscience and tissue life science 🗓️13th October 2025 🇬🇧🌐 London & online tinyurl.com/crickxrays25
tinyurl.com
Hard X-ray imaging of biological soft tissues symposium 2025
1107
Aaron Kuan @kuanawanda.bsky.social · 01/07/2025
Feeling grateful for support from @klingensteinorg.bsky.social for our research efforts, and excited to be part of this amazing cohort! 🥳🧠🔬
240
Aaron Kuan @kuanawanda.bsky.social · 12/02/2025
Latest exciting advances from @apacureanu.bsky.social: by applying self-supervised denoising, we now can resolve individual synapses with X-ray Nano-Holography (XNH), imaging through intact circuit volumes without the need for sectioning!
030