iiinova — where your digital self keeps evolving

Joyce Meiring @joycemeiri.bsky.social · 06/10/2026

You can! You just need to co-transfect the opto-katanin constructs, optimize transfection levels a bit and then use FRAP with 488nm at low power to locally illuminate every 4-30 seconds. 😉

010

Joyce Meiring @joycemeiri.bsky.social · 06/10/2026

Localized optogenetic microtubule severing in osteosarcoma cells by recruiting katanin (magenta) to microtubules (cyan).

1134

Joyce Meiring @joycemeiri.bsky.social · 06/10/2026

Optogenetic microtubule severing in a rat neuron by recruiting microtubule severing enzyme katanin (magenta) to microtubules (cyan).

150

Joyce Meiring @joycemeiri.bsky.social · 06/10/2026

Optogenetic Vimentin (magenta) pulling to the center of the cell in osteosarcoma cells with lysosomes in cyan.

121

Joyce Meiring @joycemeiri.bsky.social · 06/10/2026

Congratulations to the founders of #optogenetic ion channels in neurons for winning a nobel prize! Here's to many more decades of optogenetic experiments, that open the door to real time specific molecular manipulations and make science feel like magic! Some of my favorite movies below to celebrate.

2182

Joyce Meiring @joycemeiri.bsky.social · 24/09/2026

Excited to share that our latest story on #microtubule conformation controlling cell #signaling is now out in NCB! We show that microtubule expansion by taxane chemotherapeutics releases microtubule bound GEF-H1 which in turn activates RhoA/ROCK signaling. ✨ www.nature.com/articles/s41...

33719

Joyce Meiring @joycemeiri.bsky.social · 19/03/2026

What is the public opinion on color coding treatments in figures and then using the same colors used in the graph to outline matching representative images? Is it useful or am I being too extra?

3202

Joyce Meiring @joycemeiri.bsky.social · 09/03/2026

I collaborated with Peter Friedl's team, @jpassmore.bsky.social and @ekatrukha.bsky.social to make this short video on cancer cell invasion. Check it out! youtu.be/EH4vgUfr1jI?...

youtu.be

Invasion and Metastasis - How cancer cells become invasive

YouTube video by IMAGINE! microscopy

0133

Joyce Meiring @joycemeiri.bsky.social · 29/01/2026

Good morning to everyone except people who write "as described previously" in their paper and then cite another paper that doesn't describe the procedure. 🫠

010

Reposted by Joyce Meiring

Biomedical Picture of the Day BPoD @bpodaily.bsky.social · 21/01/2026

Tools to optically & chemically manoeuvre cytoskeleton structures intermediate filaments reveal their characteristics & interactions 📷 Milena Pasolli & @joycemeiri.bsky.social et al @utrechtuniversity.bsky.social in @jcb.org ➡️ bpod.org.uk/archive/2026... with @antdlewis.bsky.social

061

Reposted by Joyce Meiring

Eugene Katrukha 🇺🇦 @ekatrukha.bsky.social · 05/01/2026

If you have time tomorrow, join my online talk about LLS/single molecule/microtubules and 3D data at @bioimagingna.bsky.social user group (18:00 CET for Europe). I hope I manage to fit 25 movies into 20 cohesive slides and connection will be ok.

01712

Joyce Meiring @joycemeiri.bsky.social · 31/12/2025

Thanks for highlighting our most recent preprint 😊

030

Reposted by Joyce Meiring

bioRxiv Cell Biology @biorxiv-cellbio.bsky.social · 23/12/2025

Taxane-Induced Conformational Changes in the Microtubule Lattice Activate GEF-H1-Dependent RhoA Signaling www.biorxiv.org/content/10.64898/20…

001

Joyce Meiring @joycemeiri.bsky.social · 24/12/2025

Video shows paclitaxel (PTX) addition at 10 min immediately displacing GEF-H1 (left panel) from microtubules, while microtubules (right panel) remain intact and not yet strongly bundled.

230

Joyce Meiring @joycemeiri.bsky.social · 24/12/2025

Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read! Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase. www.biorxiv.org/content/10.6... Happy holidays ✨🎄

1195

Reposted by Joyce Meiring

Eugene Katrukha 🇺🇦 @ekatrukha.bsky.social · 19/09/2025

A cell finding its way through the matrix, imaged with @joycemeiri.bsky.social on LLS.

13413

Reposted by Joyce Meiring

Eugene Katrukha 🇺🇦 @ekatrukha.bsky.social · 05/09/2025

Our new preprint is about the airway epithelium microtubule network, cilia, basal body protein composition, averaging of volumetric fluorescence data, and expansion microscopy. Four years of very hard work from our very talented Emma van Grinsven. www.biorxiv.org/content/10.1... (1/N)

24116

Reposted by Joyce Meiring

Journal of Cell Biology @jcb.org · 01/09/2025

Our September issue is here! rupress.org/jcb/issue/22... Airyscan super-resolution immunofluorescence image of HeLa cells stained for two types of intermediate filaments: vimentin (magenta) & keratin-8 (cyan). From Pasolli, @joycemeiri.bsky.social, Akhmanova et al. rupress.org/jcb/article/...

0145

Joyce Meiring @joycemeiri.bsky.social · 08/08/2025

All the opto-vimentin plasmids are now available on Addgene: www.addgene.org/Anna_Akhmano...

addgene.org

Addgene: Anna Akhmanova Lab Materials

0113

Joyce Meiring @joycemeiri.bsky.social · 18/07/2025

Radial microtubules in cyan and non-radial microtubules in yellow.

030

Reposted by Joyce Meiring

Journal of Cell Biology @jcb.org · 15/07/2025

Optogenetic and chemical genetic tools for rapid repositioning of #vimentin intermediate filaments. New tools developed by Milena Pasolli, Joyce Meiring @joycemeiri.bsky.social, Anna Akhmanova and colleagues @utrechtuniversity.bsky.social: rupress.org/jcb/article/... @gijsjekoenderink.bsky.social

0183

Reposted by Joyce Meiring

Journal of Cell Biology @jcb.org · 08/07/2025

New: Pasolli, Meiring, Akhmanova et al. @utrechtuniversity.bsky.social describe tools to acutely relocate #vimentin intermediate filaments by inducibly coupling them to microtubule motors. rupress.org/jcb/article/... @joycemeiri.bsky.social @gijsjekoenderink.bsky.social #Technology #Cytoskeleton

0135

Joyce Meiring @joycemeiri.bsky.social · 08/07/2025

Here is an example of the reversibility. The first 30 min the cell is pulsed with blue light (when blue dot is shown) causing the tagged vimentin to associate with - end oriented kinesins that pull the vimentin to the cell centre. Then blue light pulsing is stopped and vimentin redistributes.

041

Joyce Meiring @joycemeiri.bsky.social · 08/07/2025

Excited to share that our fast & reversible chemical and optogenetic vimentin pulling tools are now published in JCB! 🥳 We show a subset of organelles are displaced with vimentin pulling to the cell centre and cells become much softer despite little or no effect on actin/MTs doi.org/10.1083/jcb....

1247

Reposted by Joyce Meiring

corrylab.bsky.social @corrylab.bsky.social · 16/03/2025

Thrilled to share our latest preprint from @yiechanglin.bsky.social & @ciarawallis.bsky.social showing that AF2-Multimer and AF3 can quickly & accurately predict the oligomeric states & structures of proteins (& maybe can identify multiple biologically relevant oligomeric states 👀) tiny.cc/oligomers

1368

Joyce Meiring @joycemeiri.bsky.social · 07/03/2025

Don't forget to register, abstract submission deadline is next week March 14. 😉

010

Joyce Meiring @joycemeiri.bsky.social · 19/02/2025

Is this an animated Jackson Pollock painting or live cell tracking movie? 😍

050

Joyce Meiring @joycemeiri.bsky.social · 29/01/2025

Come join us in Utrecht for some cutting edge #microscopy and 3D cell biology!! #3Dcellbio

13010

Joyce Meiring @joycemeiri.bsky.social · 27/11/2024

Hi! I was wondering if anyone had successfully cultured happy HEK293T cells in defined serum alternatives? If so, what did you use?

035

Joyce Meiring @joycemeiri.bsky.social · 22/11/2024

Excited to share our new pre-print showcasing tools (chemical & optogenetic) for repositioning Vimentin in cells! Movie shows ER (Halo-KDEL) sheets being relocalized together with vimentin (Vim) upon local vimentin pulling in U2OS. @gijsjekoenderink.bsky.social www.biorxiv.org/content/10.1...

0140

Reposted by Joyce Meiring

Christophe 🔬 Leterrier @christlet.bsky.social · 21/11/2024

Time to shine for intermediate filaments! An optogenetic vimentin perturbation strategy reveals its role for ER and mitochondria positioning (but no effect on actin or microtubules patterns) @joycemeiri.bsky.social www.biorxiv.org/content/10.1...

1339

Joyce Meiring @joycemeiri.bsky.social · 20/11/2024

CAMSAP3 stretches (microtubule -end binding protein) are shown in yellow. They move towards the Soma in developing neurons.

141

Joyce Meiring @joycemeiri.bsky.social · 20/11/2024

We like the Cytochrome C from BD Pharmingen cat#556432

020

Joyce Meiring @joycemeiri.bsky.social · 19/11/2024

This video shows the smallest area I've managed to sever inside a U2OS cell. The goal for this experiment was to sever microtubules around a subset of the Golgi (labelled here in cyan, together with exocytic vesicles) to see what would happen to it's morphology/localization.

000

Joyce Meiring @joycemeiri.bsky.social · 19/11/2024

However in a neuron it could be a smaller area still, because diffusion is slower in neurites due to their morphology. I haven't tried to see how small the area could be in neurons though.

110

Joyce Meiring @joycemeiri.bsky.social · 19/11/2024

Good question, I would estimate an area of around 10 um^2.

010

Joyce Meiring @joycemeiri.bsky.social · 19/11/2024

Hi! My name is Joyce and I love building and using tools to manipulate the #cytoskeleton of cells to better understand their function. 🔬 Below you can see my movie of #opto-katanin ✂️(SspB-mCh-p60) being used to locally disassemble #microtubules (labelled by SiR-tubulin) inside a neuron.

710418