Reposted by Jorge Lázaro
🐟 New preprint ! 🐟 For Hippo pathway aficionados.
We generated a CRISPR/Cas9 mediated single-copy endogenous Yap1-mGreenLantern fusion protein in medaka to follow Yap1 dynamics in vivo, through embryogenesis and beyond. And it is such a beautiful line ! 🧵
biorxiv.org
Spatio-temporal visualisation and manipulation of endogenous Yap1 in medaka
A key requirement to address fundamental questions in developmental biology is the ability to visualise and manipulate endogenous signalling proteins in vivo. Here we generate such an enabling experim...
