Sign in

Joey Tidei

@j-today.bsky.social
169 followers 285 following 33 posts

PhD candidate in the Beach and Oakes Labs at Loyola University Chicago. I like microscopes. I like neurons. I like bread.

PostsRepliesMedia
Joey Tidei @j-today.bsky.social · 25/09/2026
The AI ciliary sleuth himself @christlet.bsky.social!
120
Joey Tidei @j-today.bsky.social · 25/09/2026
Also a big thank you to @pjenkinslab.bsky.social for flying out to both give an awesome seminar and support me in my defense. Sorry about your Lions…
111
Joey Tidei @j-today.bsky.social · 25/09/2026
Thrilled to share that last week I successfully defended my PhD! A HUGE thank you to my mentors @myosincity.bsky.social and @pwoakes.bsky.social for the constant support/friendship over the years. Excited to continue my love of imaging as a postdoc at Northwestern's Center for Advanced Microscopy!
Defense image
2141
Reposted by Joey Tidei
Patrick Oakes @pwoakes.bsky.social · 18/08/2026
@myosincity.bsky.social and I are looking to hire a new postdoc this winter! Open to anyone broadly interested in studying how cells generate and interpret mechanical signals. Interested applicants can reach out to me and Jordan directly.
01313
Joey Tidei @j-today.bsky.social · 17/07/2026
Well it's lovely and I look forward to using it in the future 😎
010
Joey Tidei @j-today.bsky.social · 17/07/2026
Very cool! Which LUT are you using for this?
110
Joey Tidei @j-today.bsky.social · 15/06/2026
Drop a surprise challenge before they start that they’re gonna be from P2 mice…
120
Joey Tidei @j-today.bsky.social · 22/05/2026
Just me again with more neurons. Happy #FluorescenceFriday. Have a great weekend.
0242
Reposted by Joey Tidei
Christophe 🔬 Leterrier @christlet.bsky.social · 14/04/2026
It's funky axons time 🔬 🧪
211418
Joey Tidei @j-today.bsky.social · 29/03/2026
Hoping you make the frozen four so we can see you in the final 🦡🏒
110
Joey Tidei @j-today.bsky.social · 16/03/2026
The forbidden Stromboli…
030
Reposted by Joey Tidei
Spirochrome @spirochrome.com · 03/03/2026
🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪
Widefield microscope image of HeLa cells stained with SPY488-FastActWidefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)
0134
Joey Tidei @j-today.bsky.social · 27/02/2026
Up close and personal with a branching neurite in a DIV2 hippocampal neuron expressing FTractin-GFP. This was imaged every 6 seconds over 10 minutes on the trusty @zeiss-microscopy.bsky.social LSM880. Happy #FluorescenceFriday
04711
Joey Tidei @j-today.bsky.social · 25/02/2026
Also looks like it has some actin within it as well. You've really got me wondering now 😂
120
Joey Tidei @j-today.bsky.social · 25/02/2026
Although the final frame looks like it may have some attachment, or just a coincidental new branch forming in the same area.
010
Joey Tidei @j-today.bsky.social · 25/02/2026
That's a great question, I have no idea lol. I'm only collecting one z-plane here so its possible if its a neuronal structure I could be missing its attachment to the neighboring neurite.
020
Joey Tidei @j-today.bsky.social · 25/02/2026
Thank you!!
000
Joey Tidei @j-today.bsky.social · 24/02/2026
SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!
3104
Joey Tidei @j-today.bsky.social · 20/02/2026
Thank you! And I bet you have some idea.... ol' reliable. SiR-Tubulin and SPY-FastActX 👍
110
Joey Tidei @j-today.bsky.social · 20/02/2026
Cell body getting taken for a ride in this early stage neuron. Microtubules🔵 and Actin🟣 labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday
25611
Reposted by Joey Tidei
Maggie Utgaard @maggieutgaard.bsky.social · 23/01/2026
So excited to share the preprint with the bulk of my PhD work in the Beach @myosincity.bsky.social and Oakes @pwoakes.bsky.social Labs at Loyola University Chicago! We investigate the structure, recruitment, and function of septin networks around the nucleus. A thread: doi.org/10.64898/202...
doi.org
22915
Reposted by Joey Tidei
Elias Spiliotis @septinlab.bsky.social · 21/01/2026
Two new 🔥pre-prints 🔥 TODAY by us & @pwoakes.bsky.social @myosincity.bsky.social change the way we think about the septin cytoskeleton Septins buffer mechanical stress on actin and membranes! Septins are like intermediate filaments www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6...
media.tenor.com
a woman in a suit and tie has the words wait what written on her face
ALT: a woman in a suit and tie has the words wait what written on her face
02813
Joey Tidei @j-today.bsky.social · 19/12/2025
Thank you!
010
Joey Tidei @j-today.bsky.social · 19/12/2025
I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy
212127
Joey Tidei @j-today.bsky.social · 01/08/2025
Microtubules 🟢 protruding into the dynamic actin 🟣 network of a neuronal growth cone in DIV2 hippocampal neurons. @spirochrome.com SiR-Tubulin and SPY-FastActX working wonders on this #FluorescenceFriday
17310
Reposted by Joey Tidei
Patrick Oakes @pwoakes.bsky.social · 21/07/2025
Our new preprint is out! Led by @stefanosala89.bsky.social sky.social we investigate the difference between molecular tension sensing and global tension on the focal adhesion. Follow along for a thread of our findings: www.biorxiv.org/content/10.1...
biorxiv.org
LIM Domain Proteins link molecular and global tension by recognizing strained actin in adhesions
Mechanotransduction is fundamental to cell signaling and depends on force-sensitive adhesion proteins. How these proteins differentiate and integrate their responses to tension remains an open questio...
23710
Reposted by Joey Tidei
Ana Dorrego Rivas @adorrego.bsky.social · 10/07/2025
A day ahead of #FluorescenceFriday, a (temporal) switch from the bulb to the cortex. 🟣 NeuN 🔵 AnkG 🔬🧠🧪
1474
Joey Tidei @j-today.bsky.social · 09/05/2025
Imaging neurons quickly for 3min typically won't give you huge cell-wide cytoskeletal events, but you do get to capture fun little tugs and loops in the soma and neurites (as well as whatever the hell is going on in the bottom right). Stain: @spirochrome.com SiR-Tubulin Happy #FlourescenceFriday!
1122
Joey Tidei @j-today.bsky.social · 05/05/2025
Did you wake up this morning and think "Huh, I'd love to learn more about how myosin 2 filaments assemble and their contribution to cell architecture and function"? What a coincidence, I've got just the review for you.
sciencedirect.com
Myosin 2 – A general contractor for the cytoskeleton
Cells derive their shape, and in turn much of their behavior, from the organization of the cytoskeleton. While a myriad of proteins contribute to the …
091
Reposted by Joey Tidei
Christophe 🔬 Leterrier @christlet.bsky.social · 17/04/2025
Our PhD student Louis Romette is optimizing long-term live-cell imaging. He just casually dropped this 65-hour movie of a growing neuron (div 3 to 6) with knocked-in actin 🔥🕶️ #realtimemicroscopy
1429950
Joey Tidei @j-today.bsky.social · 17/04/2025
Beautiful! What imaging media did you wind up going with?
030
Reposted by Joey Tidei
Elias Spiliotis @septinlab.bsky.social · 16/04/2025
Two months left for FASEB Conference - Septins: Spatial regulators of cell biology Register now: events.faseb.org/event/septin... Come find out what these filaments do at the interface of the actin and microtubule cytoskeletons and cell membranes. You'll be hooked for life.
0115
Joey Tidei @j-today.bsky.social · 18/03/2025
Thank you @daniellebeckman.bsky.social ! Obviously the same for all of your images!
110
Reposted by Joey Tidei
MBF Bioscience @mbfbioscience.bsky.social · 18/03/2025
Congrats to the NeuroArt Grand Prize winners! 🥇 Totally Tubular –Joey Tidei @j-today.bsky.social 🥈 Neurosphere – Adam Soos 🥉 Unwelcome Guest – Danielle Beckman @daniellebeckman.bsky.social Thanks to all who shared their art! Want to be next? Enter our contest! 🔗 neuroart.com/annual-grand...
1203
Reposted by Joey Tidei
Kevin Terretaz @kwolbachia.bsky.social · 14/03/2025
For this #FluorescenceFriday I share another example of the possibilities of making art with imageJ processing. This is a 3 day long time-lapse of a growing slime mold, with 15 min growth waves color coded. the white things are oat flakes. I'll share the original video in comments #Sciart
2399
Joey Tidei @j-today.bsky.social · 14/03/2025
Almost forgot to mention.... @kwolbachia.bsky.social Citynight LUT strikes again!
110
Joey Tidei @j-today.bsky.social · 14/03/2025
I've been lacking on #FluorescenceFriday the past month or two, but winning the annual MBF NeuroArt competition was a lovely surprise to end the week. Thank you @mbfbioscience.bsky.social for the recognition! neuroart.com/annual-grand...
1101
Joey Tidei @j-today.bsky.social · 27/01/2025
Unfortunately it is. Happy to see ideas being thrown around here though lol
010
Joey Tidei @j-today.bsky.social · 27/01/2025
I do most of my troubleshooting imaging with NBM/B27 but if I need good clear imaging I would use NEUMO/SOS. I'll have to check but I believe the longest I did with that media was overnight and it was definitely reduced autofluorescence. Relatively new, now relatively old I guess.
110
Joey Tidei @j-today.bsky.social · 27/01/2025
Following since I've just been informed NEUMO/SOS is discontinued 😧
240
Joey Tidei @j-today.bsky.social · 21/01/2025
The world desperately needs more microscope merch. I’d spend half my stipend.
010
Joey Tidei @j-today.bsky.social · 17/01/2025
Zoomed in on the base of an axon looking at some funky actin dynamics for this #FluorescenceFriday
0282
Joey Tidei @j-today.bsky.social · 05/01/2025
I’ve never had luck with warm proofs. Cold proof overnight always gives me great spring 👍
110
Reposted by Joey Tidei
Christophe 🔬 Leterrier @christlet.bsky.social · 02/01/2025
#TIRFSIMThursday with a COS cell labeled for actin, clathrin and microtubules
310112
Reposted by Joey Tidei
Patrick Oakes @pwoakes.bsky.social · 12/12/2024
It'll be a slightly smaller crew than previous years but we'll still be at #cellbio24 #ASCB! If you like LIM proteins or septins come check out our posters:
0167
Joey Tidei @j-today.bsky.social · 22/11/2024
This place really is bumpin’… My first #FluorescenceFriday on Bluesky. Recycled, but a DIV4 rat hippocampal neuron expressing a probe for F-Actin and imaged on an LSM880 with Airyscan.
0405