Joey Tidei @j-today.bsky.social · 25/09/2026The AI ciliary sleuth himself @christlet.bsky.social! 120
Joey Tidei @j-today.bsky.social · 25/09/2026Also a big thank you to @pjenkinslab.bsky.social for flying out to both give an awesome seminar and support me in my defense. Sorry about your Lions… 111
Joey Tidei @j-today.bsky.social · 25/09/2026Thrilled to share that last week I successfully defended my PhD! A HUGE thank you to my mentors @myosincity.bsky.social and @pwoakes.bsky.social for the constant support/friendship over the years. Excited to continue my love of imaging as a postdoc at Northwestern's Center for Advanced Microscopy! 2141
Reposted by Joey TideiPatrick Oakes @pwoakes.bsky.social · 18/08/2026@myosincity.bsky.social and I are looking to hire a new postdoc this winter! Open to anyone broadly interested in studying how cells generate and interpret mechanical signals. Interested applicants can reach out to me and Jordan directly. 01313
Joey Tidei @j-today.bsky.social · 17/07/2026Well it's lovely and I look forward to using it in the future 😎 010
Joey Tidei @j-today.bsky.social · 15/06/2026Drop a surprise challenge before they start that they’re gonna be from P2 mice… 120
Joey Tidei @j-today.bsky.social · 22/05/2026Just me again with more neurons. Happy #FluorescenceFriday. Have a great weekend. 0242
Reposted by Joey TideiChristophe 🔬 Leterrier @christlet.bsky.social · 14/04/2026It's funky axons time 🔬 🧪 211418
Joey Tidei @j-today.bsky.social · 29/03/2026Hoping you make the frozen four so we can see you in the final 🦡🏒 110
Reposted by Joey TideiSpirochrome @spirochrome.com · 03/03/2026🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪 0134
Joey Tidei @j-today.bsky.social · 27/02/2026Up close and personal with a branching neurite in a DIV2 hippocampal neuron expressing FTractin-GFP. This was imaged every 6 seconds over 10 minutes on the trusty @zeiss-microscopy.bsky.social LSM880. Happy #FluorescenceFriday 04711
Joey Tidei @j-today.bsky.social · 25/02/2026Also looks like it has some actin within it as well. You've really got me wondering now 😂 120
Joey Tidei @j-today.bsky.social · 25/02/2026Although the final frame looks like it may have some attachment, or just a coincidental new branch forming in the same area. 010
Joey Tidei @j-today.bsky.social · 25/02/2026That's a great question, I have no idea lol. I'm only collecting one z-plane here so its possible if its a neuronal structure I could be missing its attachment to the neighboring neurite. 020
Joey Tidei @j-today.bsky.social · 24/02/2026SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info! 3104
Joey Tidei @j-today.bsky.social · 20/02/2026Thank you! And I bet you have some idea.... ol' reliable. SiR-Tubulin and SPY-FastActX 👍 110
Joey Tidei @j-today.bsky.social · 20/02/2026Cell body getting taken for a ride in this early stage neuron. Microtubules🔵 and Actin🟣 labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday 25611
Reposted by Joey TideiMaggie Utgaard @maggieutgaard.bsky.social · 23/01/2026So excited to share the preprint with the bulk of my PhD work in the Beach @myosincity.bsky.social and Oakes @pwoakes.bsky.social Labs at Loyola University Chicago! We investigate the structure, recruitment, and function of septin networks around the nucleus. A thread: doi.org/10.64898/202...doi.org 22915
Reposted by Joey TideiElias Spiliotis @septinlab.bsky.social · 21/01/2026Two new 🔥pre-prints 🔥 TODAY by us & @pwoakes.bsky.social @myosincity.bsky.social change the way we think about the septin cytoskeleton Septins buffer mechanical stress on actin and membranes! Septins are like intermediate filaments www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6...media.tenor.coma woman in a suit and tie has the words wait what written on her faceALT: a woman in a suit and tie has the words wait what written on her face 02813
Joey Tidei @j-today.bsky.social · 19/12/2025I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy 212127
Joey Tidei @j-today.bsky.social · 01/08/2025Microtubules 🟢 protruding into the dynamic actin 🟣 network of a neuronal growth cone in DIV2 hippocampal neurons. @spirochrome.com SiR-Tubulin and SPY-FastActX working wonders on this #FluorescenceFriday 17310
Reposted by Joey TideiPatrick Oakes @pwoakes.bsky.social · 21/07/2025Our new preprint is out! Led by @stefanosala89.bsky.social sky.social we investigate the difference between molecular tension sensing and global tension on the focal adhesion. Follow along for a thread of our findings: www.biorxiv.org/content/10.1...biorxiv.orgLIM Domain Proteins link molecular and global tension by recognizing strained actin in adhesionsMechanotransduction is fundamental to cell signaling and depends on force-sensitive adhesion proteins. How these proteins differentiate and integrate their responses to tension remains an open questio... 23710
Reposted by Joey TideiAna Dorrego Rivas @adorrego.bsky.social · 10/07/2025A day ahead of #FluorescenceFriday, a (temporal) switch from the bulb to the cortex. 🟣 NeuN 🔵 AnkG 🔬🧠🧪 1474
Joey Tidei @j-today.bsky.social · 09/05/2025Imaging neurons quickly for 3min typically won't give you huge cell-wide cytoskeletal events, but you do get to capture fun little tugs and loops in the soma and neurites (as well as whatever the hell is going on in the bottom right). Stain: @spirochrome.com SiR-Tubulin Happy #FlourescenceFriday! 1122
Joey Tidei @j-today.bsky.social · 05/05/2025Did you wake up this morning and think "Huh, I'd love to learn more about how myosin 2 filaments assemble and their contribution to cell architecture and function"? What a coincidence, I've got just the review for you.sciencedirect.comMyosin 2 – A general contractor for the cytoskeletonCells derive their shape, and in turn much of their behavior, from the organization of the cytoskeleton. While a myriad of proteins contribute to the … 091
Reposted by Joey TideiChristophe 🔬 Leterrier @christlet.bsky.social · 17/04/2025Our PhD student Louis Romette is optimizing long-term live-cell imaging. He just casually dropped this 65-hour movie of a growing neuron (div 3 to 6) with knocked-in actin 🔥🕶️ #realtimemicroscopy 1429950
Joey Tidei @j-today.bsky.social · 17/04/2025Beautiful! What imaging media did you wind up going with? 030
Reposted by Joey TideiElias Spiliotis @septinlab.bsky.social · 16/04/2025Two months left for FASEB Conference - Septins: Spatial regulators of cell biology Register now: events.faseb.org/event/septin... Come find out what these filaments do at the interface of the actin and microtubule cytoskeletons and cell membranes. You'll be hooked for life. 0115
Joey Tidei @j-today.bsky.social · 18/03/2025Thank you @daniellebeckman.bsky.social ! Obviously the same for all of your images! 110
Reposted by Joey TideiMBF Bioscience @mbfbioscience.bsky.social · 18/03/2025 Congrats to the NeuroArt Grand Prize winners! 🥇 Totally Tubular –Joey Tidei @j-today.bsky.social 🥈 Neurosphere – Adam Soos 🥉 Unwelcome Guest – Danielle Beckman @daniellebeckman.bsky.social Thanks to all who shared their art! Want to be next? Enter our contest! 🔗 neuroart.com/annual-grand... 1203
Reposted by Joey TideiKevin Terretaz @kwolbachia.bsky.social · 14/03/2025For this #FluorescenceFriday I share another example of the possibilities of making art with imageJ processing. This is a 3 day long time-lapse of a growing slime mold, with 15 min growth waves color coded. the white things are oat flakes. I'll share the original video in comments #Sciart 2399
Joey Tidei @j-today.bsky.social · 14/03/2025Almost forgot to mention.... @kwolbachia.bsky.social Citynight LUT strikes again! 110
Joey Tidei @j-today.bsky.social · 14/03/2025I've been lacking on #FluorescenceFriday the past month or two, but winning the annual MBF NeuroArt competition was a lovely surprise to end the week. Thank you @mbfbioscience.bsky.social for the recognition! neuroart.com/annual-grand... 1101
Joey Tidei @j-today.bsky.social · 27/01/2025Unfortunately it is. Happy to see ideas being thrown around here though lol 010
Joey Tidei @j-today.bsky.social · 27/01/2025I do most of my troubleshooting imaging with NBM/B27 but if I need good clear imaging I would use NEUMO/SOS. I'll have to check but I believe the longest I did with that media was overnight and it was definitely reduced autofluorescence. Relatively new, now relatively old I guess. 110
Joey Tidei @j-today.bsky.social · 27/01/2025Following since I've just been informed NEUMO/SOS is discontinued 😧 240
Joey Tidei @j-today.bsky.social · 21/01/2025The world desperately needs more microscope merch. I’d spend half my stipend. 010
Joey Tidei @j-today.bsky.social · 17/01/2025Zoomed in on the base of an axon looking at some funky actin dynamics for this #FluorescenceFriday 0282
Joey Tidei @j-today.bsky.social · 05/01/2025I’ve never had luck with warm proofs. Cold proof overnight always gives me great spring 👍 110
Reposted by Joey TideiChristophe 🔬 Leterrier @christlet.bsky.social · 02/01/2025#TIRFSIMThursday with a COS cell labeled for actin, clathrin and microtubules 310112
Reposted by Joey TideiPatrick Oakes @pwoakes.bsky.social · 12/12/2024It'll be a slightly smaller crew than previous years but we'll still be at #cellbio24 #ASCB! If you like LIM proteins or septins come check out our posters: 0167
Joey Tidei @j-today.bsky.social · 22/11/2024This place really is bumpin’… My first #FluorescenceFriday on Bluesky. Recycled, but a DIV4 rat hippocampal neuron expressing a probe for F-Actin and imaged on an LSM880 with Airyscan. 0405