High-resolution cryo-EM structures of S. aureus ABC transporter PmtCD in apo and ADP-bound states in multiple membrane mimetics define a large intramembrane lumen, adding insight to the export of viru...

Phosphatidylinositol 4 kinase IIIα (PI4KIIIα/PI4KA) is an essential lipid kinase that plays a critical role in regulating plasma membrane identity. PI4KA is primarily recruited to the plasma membrane ...

Study reveals how cholesterol-lowering drugs can trigger muscle damage—and point to a way to make them safer.

Statins lower blood plasma cholesterol but can cause muscle-related issues including life-threatening rhabdomyolysis. Here the authors show that atorvastatin binds as triplets to the skeletal muscle R...

The phosphorylation of the histone variant H2AX on the nucleosome, yielding γH2AX, acts as a 'master control switch', signaling the recruitment of DNA repair factors at DNA double-stranded break sites. This phosphorylation is recognized by BRCA1 carboxy-terminal (BRCT) domains of specific repair pro …

Long-circulating, transfection-competent LNP-mRNA systems are key for effective extrahepatic delivery. Here, authors show that LNPs with high bilayer lipid ratios yield high mRNA encapsulation, prolon...

This Review discusses the diversity of cell envelope architectures across major bacterial phyla and highlights conserved features found in diderm lineages.

Lipid nanoparticle (LNP) formulations are a proven method for the delivery of nucleic acids for gene therapy as exemplified by the worldwide rollou...

SBGrid webinars are hosted with partial support from the NIH R25 Continuing Education for Structural Biology Mentors #GM151273, in collaboration with Co-PI J...

KCNQ1 channels are active in heart, brain and gut. Functional loss causes epilepsy and sudden arrhythmic death. Here, authors describe a key activator drug binding site, explaining isoform and drug se...

Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the phosphatidylinositol 4-phosphate (PI4P) and phosphatidylserine pools of the plasma membrane.…

The use of native mass spectrometry (MS) to land biological molecules for subsequent cryogenic electron microscopy (cryoEM) imaging and three-dimensional reconstruction has gained momentum in recent y...

A step-by-step Cryo-ET guide

ABCA4 is an ATP-binding cassette (ABC) transporter that flips N-retinylidenephosphatidylethanolamine (N-Ret-PE) to the cytoplasmic leaflet of photoreceptor membranes. ABCA4 mutations are associated wi...

The presence of a cell membrane is one of the major structural components defining life. Recent phylogenomic analyses have supported the hypothesis...

Lipid nanoparticles (LNPs) containing ionizable cationic lipids are proven delivery systems for therapeutic nucleic acids, such as small interfering R…

After years of frustration, researchers learn how to sharpen molecular images with liquid helium

Ryanodine Receptor type 3 mediates ER Ca2+ release in different cell types. Here, the authors use cryo-EM to reveal a binding site for chloride and two distinct sites for ATP. Epileptic encephalopathy...

Rab3GAP, a guanine nucleotide exchange factor for Rab18, regulates membrane trafficking and lipid droplet metabolism. The authors elucidated its molecular structure, mapped its potential substrate bin...

Cryo-EM illuminates atomic binding details and far-ranging conformational effects of the inhibitor targocil-II on wall teichoic acid ABC transporter TarGH, a virulence target from the antibiotic resis...

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution