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Phoebe Glazer @glazerchemistrylab.bsky.social · 20/12/2024

The Department of Chemistry at North Carolina State University! @ncstatechem.bsky.social

Phoebe Glazer @glazerchemistrylab.bsky.social · 11/12/2024

Your “Structure of an R01” was the best grant writing guide I had as a new (and unconnected) Assistant Professor. I have used it in classes I teach and shared it with many Jr colleagues. Thank you for demystifying the system for many of us and helping to make the process more accessible to all.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 02/12/2024

Finally, we show why most work with Seahorse should be done at low concentrations and early time points. Meaning, working below the cytotoxicity IC50 values, and after only short incubations (1-2 hrs). Otherwise, you get misleading data. We suggest best practices for researchers to avoid pitfalls.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 02/12/2024

Glycolysis increases the acidity of the tumor microenvironment (TME), and this is generally believed to be immunosuppressive. Thus, PDT may reduce the immunosuppressive TME, encouraging combination of PDT with immunotherapies.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 02/12/2024

This could provide a strong rationale for PDT fractionation, as PDT requires O2, and there will be MORE O2 available when OXPHOS is inhibited. It might be possible to get more treatment bang for the photon buck with fractionated PDT treatments.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 02/12/2024

This is great news is you want to use photocages and not alter metabolic processes! And it has real implications for PDT: PDT induced metabolic reprograming, with OXPHOS and glycolysis shut down by the PDT photosensitizes. This indicates that PDT and might synergize with metabolic disruptors.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 02/12/2024

Many groups are using the Seahorse Metabolic Analysis to study compounds. We investigated the impact of light-activated molecules on oxidative phosphorylation and glycolysis. We found that Ru(II) PDT photosensitizes have a significant impact on metabolism. Ru(II) photocages do not.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 21/11/2024

These include working below the cytotoxicity IC50 values, and after only short incubations (1-2 hrs). Otherwise, what is observed may be a downstream effect of other biological processes.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 21/11/2024

What are the implications? PDT may be effective for metabolic reprograming and might synergize with metabolic disruptors. This could also have implications for combination PDT with immunotherapies. PACT agents can be used as photocages without disrupting metabolism. We also suggest best practices.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 21/11/2024

We found two thing: 1) PDT agents shut down both OXPHOS and glycolysis, and induce senescence. PACT agents do not. 2) It is essential to do time and concentration dependent studies, and most work should be done at low concentrations and early time points. Otherwise, you get misleading data.

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Phoebe Glazer @glazerchemistrylab.bsky.social · 21/11/2024

Sharing some info on our latest paper coming out soon in Chemical Science. 🧵 Many groups are using the Seahorse Metabolic Analysis to study compounds. We investigated the impact of light-activated molecules on oxidative phosphorylation and glycolysis by comparing PDT agents and photocages (PACT).

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Reposted by Phoebe Glazer

Thorn-Seshold Lab @thornsesholdlab.bsky.social · 17/11/2024

📯📯 Chemical Biology starter pack 📯📯 140 PIs & groups posting primary research in chemical biology. Please reskeet (?) & reply to this post if you want to be added! go.bsky.app/KLrTWoj Let's make ChemBioSky even greater again! #realtimechem #FluorescenceFriday #chemsky #chemtwitter #AgentOrange

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Reposted by Phoebe Glazer

Razvan Borza @rborza.bsky.social · 17/11/2024

⚠️THREAD OF ALL SCIENCE-RELATED STARTER PACKS! ⚠️ 🔄Share to help people finding its topic/field - STRUCTURAL BIOLOGY Structural Biology 1: bsky.app/starter-pack... Structural Biology 2: bsky.app/starter-pack... Crystallography: bsky.app/starter-pack... Synchrotron&CryoEM: bsky.app/starter-pack...

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Phoebe Glazer @glazerchemistrylab.bsky.social · 16/11/2024

Yay, thank you Catherine, this is great!!! Now we just need to get more of the community to migrate over here!

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Phoebe Glazer @glazerchemistrylab.bsky.social · 15/11/2024

Twitter used to be fun, X got to be not so fun, I am hoping Bluesky will provide a good medium for scientific community!

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