In an effort to make Oligopaint FISH probes more broadly available to the research community, we offer DNA and RNA FISH probe design, synthesis, and generation. By dedicating a full-time staff memb…

Cohesin loop extrusion is a major driver of chromosome folding, but how its dynamics are controlled to shape the genome remains elusive. Here we disentangle the contributions of the cohesin cofactors ...

The nuclear periphery is a key site for heterochromatin organization in eukaryotic cells, where lamina-associated domains (LADs) promote transcriptional repression and genome stability. Despite their ...

Author summary The three-dimensional organization of the genome plays a critical role in regulating cellular function, yet the mechanisms that govern large-scale chromatin architecture during…

Thomas, Feng, et al. introduce a synthetic platform that allows the building of complex regulatory landscapes. Integrating the same enhancer at different distances from a promoter uncovered that activ...

Sexual reproduction relies on robust quality control during meiosis. Assembly of the synaptonemal complex between homologous chromosomes (synapsis) regulates meiotic recombination and is crucial for a...

Harke et al. use sequential DNA-FISH to achieve single-allele resolution of the Sonic hedgehog (Shh) regulatory domain in the mouse forebrain. They show that multiple allelic configurations occur inde...