iiinova — where your digital self keeps evolving

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Erik A. Rodriguez @erin-rod.bsky.social · 29/08/2025

🌊The beautiful fluorescence of a sea anemone!🌊 #FluorescenceFriday #FluorescentProtein #Coral #Sea #Ocean

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Danielle Beckman @daniellebeckman.bsky.social · 16/08/2025

This is a direct immunostaining using the frontal lobe of a patient with Alzheimer. Why is there so little high-resolution microscopy being performed in human tissue? Question for pathologists. We can extract a lot of data, and in 3D! (Dream Theater sounds appropriate this time) #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 08/08/2025

Check this neuron infected with #SARSCoV2 being attacked by a microglia. Is this process beneficial or detrimental? Do we want therapies to inhibit microglia activation/neuroinflammation, but what if it means more neurons infecting other neurons? Neurovirology is fascinating! #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 01/08/2025

Analyzing how neurons🔵 and microglia🟡 (brain immune cells) interact and I vote for 3D microscopy videos with appropriate soundtracks 🤘 👩‍🎤🔬 #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 01/08/2025

Original image from the microscope

Confocal microscopy image showing neurons and microglia

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Matthias Böddener @mb1106.bsky.social · 25/07/2025

Indirect immunostaining of astrocytes surrounding a blood vessel in the mouse brain with GFAP 🟠. Nuclei have been visualized by DAPI staining 🔵. #bloodbrainbarrier #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 25/07/2025

Neurons on fire 🔥 ? Check how these microglia 🟡 interact with neurons 🔵 accumulating phospho-Tau 🔴, one of the hallmarks in #Alzheimer's disease. What is happening there? Are these microglia making things better or worse? #FluorescenceFriday #Neuroinflammation

Confocal microscopy image showing microglia interacting with neurons accumulating pathological tau protein in Alzheimer's disease

Confocal microscopy image showing microglia interacting with neurons accumulating pathological tau protein in Alzheimer's disease (microglia IBA-1 channel only)

Confocal microscopy image showing microglia interacting with neurons accumulating pathological tau protein in Alzheimer's disease (neurons MAP2 and phospho-tau AT8 channels only)

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Ashley Kalinski @kalinskilab.bsky.social · 18/07/2025

This #FluorescenceFriday we have adult DRG neurons cultured in microfluidics! Axons traveled through ~640um length microgrooves to the next compartment. Cyan is neurofilament. These were cultured/imaged by our super talented postbac, Jess. (who is looking for lab tech/grad positions in NJ/NY)

Blue axons growing through microgrooves that separate their cell bodies from axons.

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Danielle Beckman @daniellebeckman.bsky.social · 18/07/2025

Just pretty or useful? SMI32 🟡 targets a set of neurons more vulnerable to dementias. NeuN 🔵 identifies most mature neurons in the cortex. Quantifying neurons identified by both markers can give you insights into neurodegenerative processes occurring in the brain #Neuroscience #FluorescenceFriday

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Wohl Cellular Imaging Centre 🔬 @wohlmicroscopy.bsky.social · 18/07/2025

Every few months we vote on which of the images we post here is best - Congratulations @lodemwingie.bsky.social for winning this round with Lumbar Spinal Cord. #FluorescenceFriday

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Stephanie Huang @stephjhuang.bsky.social · 18/07/2025

Is it just me, or do the thorny excrescences on CA3 neurons remind you of wasabi roots? Anyway, happy #fluorescencefriday !

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Danielle Beckman @daniellebeckman.bsky.social · 04/07/2025

Neurons are for sure the fireworks in the brain! But these are lighting up because they are dying...by accumulating pathological tau, one of the neurodegenerative hallmarks in Alzheimer's and other dementias. Markers: pS 422 tau🔴AT8🟡NeuN🔵 #4thofJuly #FluorescenceFriday 🌠🔬

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Danielle Beckman @daniellebeckman.bsky.social · 27/06/2025

Amyloid-beta: Villain or Hero? Aβ can act as an antimicrobial peptide by trapping pathogens like bacteria, fungi & viruses in extracellular fibrillar proteins. But chronically, it can lead to neuroinflammation & degeneration. Image: Human brain with #Alzheimer. Aβ 🔵 astrocytes 🟡 #FluorescenceFriday

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Nat Prunet @nat-prunet.bsky.social · 27/06/2025

Incredible diversity of #pollen grains! Pollen autofluorescence acquired with @zeiss-microscopy.bsky.social #superresolution Airyscan & color-coded for depth #microscopy #bioimaging #bioart #sciart #botany #plantscience #fluorescencefriday

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Keri Martinowich @martinowk.bsky.social · 13/06/2025

For #FluorescenceFriday - really pretty image from fresh off the scope experiments to identify basolateral amygdala - lateral septum projection neurons 🟢 that are recruited in response to social cues 🔴 in 🐁🧠. Image credit to postdoc @rphillips3.bsky.social and summer RA Natalia Gonzalez Perez 🔬🧪👩‍🔬

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Danielle Beckman @daniellebeckman.bsky.social · 13/06/2025

How do the two most abundant glial cell types in the brain "welcome" red blood cells and other things coming through the blood vessels? Check the image below! 🤓 🔬 🧠 Astrocytes 🔵 Microglia 🟡 Red blood cells 🔴 #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 07/06/2025

Today is my birthday and of course it's #FluorescenceFriday 🔬! My mother was going to come and spend the day with me, but life had other plans💔. If you can, share a photo you took, with a microscope, telescope or cell phone! 100% AI-Free! Microglia🔵 & Neurons🟡 in the hippocampus in the image below!

Confocal microscopy photo of the hippocampus showing neurons in yellow and microglia in blue

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Matthias Böddener @mb1106.bsky.social · 06/06/2025

Immunostaining of a mouse brain (hippocampus) section with GluT1 🟣 and ALDH1L1 🟢. Nuclei have been visualized by DAPI staining 🔵. #bloodbrainbarrier #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 23/05/2025

How does a white matter lesion look like under the confocal microscope 🤓? You can see a blood vessel going bad in the left corner. These lesions are often associated with age and cardiovascular risk factors, but can also be found in young adults. Neurons🔵, astrocytes🔴, microglia🟡 #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 09/05/2025

Astrocytes🔵 reacting to B-amyloid🟣 accumulation in the Human Neocortex. One of the hallmarks of #Alzheimers and other neurodegenerative conditions. What if viral infections increase the accumulation of these and other misfolded proteins in the brain? 🤔🔬 #FluorescenceFriday

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Danielle Beckman @daniellebeckman.bsky.social · 02/05/2025

My mom used to love #FluorescenceFriday, so I will keep posting in her memory ❤️ This photo shows neurons in the hippocampus accumulating phospho-tau in our monkey model of Alzheimer's Disease. Markers: Dapi 🔵, Neun 🔴, pTau 422🟣, AT8🟡

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Keri Martinowich @martinowk.bsky.social · 25/04/2025

For #FluorescenceFriday - pretty image of ventral tegmental area (VTA) in the mouse 🧠 labeled with tyrosine hydroxylase (TH) 🟣. Red and green are from experiments trying to virally label various projection neurons targeting the brainstem and midbrain 🔬🧪

Microscopy image at the level of the ventral tegmental area in mouse brain, showing dopamine cells in the VTA labeled in purple with tyrosine hydroxylase

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Ana Dorrego Rivas @adorrego.bsky.social · 04/04/2025

A dopaminergic neuron with no axon, its dendrites stretching like branches, both message and messenger. Inverted from fluorescent green to monochrome, revealing the art within structure. #FluorescenceFriday 🧠🧪🔬

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Sydney Floryanzia 👑 @neurochemequeen.bsky.social · 28/03/2025

A new #FluorescenceFriday for today! ✨🔬🧠 My 1st time assembling a full #BloodBrainBarrier in a #microfluidic device. 🔴 Claudin 5 (endothelial protein) 🟡 ZO-1 (endothelial protein) 🟢 GFAP (astrocytes) 🔵 DAPI (cell nuclei) 🟣 NG2 (pericytes) #neuroskyence #neuroscience #bbb #microscopy #confocal

A multicolored confocal microscopy image with blood-brain barrier cells in a microfluidic device on a black background. Red colored endothelial cells are in the left channel, green astrocytes, and purple pericytes are in the right channel. The channels are connected by small openings

Confocal microscopy image of endothelial cells highlighting their tight junction proteins in yellow (ZO-1) and red (Claudin 5) on a black background

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Diana Brychka @dianabrychka.bsky.social · 28/03/2025

My little brain organoid with stained neurons ❤️ and nuclei 🤍 #FluorescenceFriday #SciArt

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Chris Langsdorf @fluorist.bsky.social · 21/03/2025

🌷Spring flowers? Fireworks? Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System. 🧠🔬🧪 #Neuroscience #FluorescenceFriday

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Keri Martinowich @martinowk.bsky.social · 07/03/2025

For #FluorescenceFriday - ventral tegmental area (VTA) of human 🧠, labeling neurons expressing tyrosine hydroxylase 🟡, a marker for dopamine synthesis. VTA dopamine neurons are critical for reward processing and motivation, and their dysfunction is implicated in addiction and depression 🧪🔬

RNAscope in situ hybridization probing for the enzyme Tyrosine Hydroxylase, which is important for dopamine synthesis. Dopamine neurons are labeled here in yellow on this section of human brain tissue at the level of the ventral segmental area.

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Viroscope @viroscope.bsky.social · 07/03/2025

A Spiky Cell A boa constrictor brain-derived cell stained for membranes (cyan), actin (yellow) and DNA (pink) #FluorescenceFriday #SciArt

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Stacey Ogden 🦔 @labogden.bsky.social · 28/02/2025

Happy #FluorescenceFriday! Here is an E10.5 transgenic mouse embryo showing expression of my favorite gene, Sonic Hedgehog, in blue! Microscopy by former lab #DevBio researcher Tina Zhang 🧪 👩‍🔬 🔬

Image of a Shh promoter-driven lineage tracer (blue) expressed in an E10.5 mouse. The Shh gene expresses along the neural tube and gut at this developmental stage. A membrane marker is shown in green to highlight the rest of the embryo.

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Tallie Z Baram @talliezee.bsky.social · 21/02/2025

So, stress amplifies our coping strategies: Colocalization of the 2 stress-sensitive peptides, CRH (green) and vasopressin (red), is augmented in adult hypothalamus after early-life stress #FluorescenceFriday 🧪🧠 #NeurobiologyofStress

a part of the brain charged with responding to stress contains 2 mediators, red and green, typically in separate cells. early-life stress leads to their presence in a single cell, perhaps amplifying responses to stress later in life

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Rachael Ott @rachaelott.bsky.social · 21/02/2025

For #FluorescenceFriday, microtubules are shown in this massive oligodendrocyte from a primary hippocampal culture 🔬 #Microscopy #Neuroscience

Microtubules (green; α-tubulin) are shown in a primary oligodendrocyte.

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Christophe 🔬 Leterrier @christlet.bsky.social · 21/02/2025

A classic for #FluorescenceFriday with a cultured neuron labeled for actin (red), ß2-spectrin (green) and map2 (blue) 🧪

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Jana Kroll @janakroll.bsky.social · 21/02/2025

Cryo-confocal microscopy of a neuron expressing the glutamate sensor iGluSnFR 🥶 The neuron was optogenetically stimulated and subsequently plunge-frozen to preserve the high fluorescence intensity indicating neurotransmitter release. #FluorescenceFriday

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Keri Martinowich @martinowk.bsky.social · 12/02/2025

The locus coeruleus is located in the brainstem and is among the first regions in the human 🧠 to acquire pathology in Alzheimer's disease. Loss of neurons in the locus coeruleus is directly linked to cognitive decline. 🟢TH labels noradrenergic neurons; 🟣 pTau labels neurofibrillary tangles 🔬👩‍🔬🧪

Staining in green (TH) to label noradrenergic neurons and magenta (pTau) in a tissue section through the locus coeruleus in the human brain

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Danielle Beckman @daniellebeckman.bsky.social · 31/01/2025

Blood vessels arriving in the brain for this #FluorescenceFriday! Major components of the 'blood-brain barrier' are in this photo. This 'barrier' is just a bunch of cells that can be hijacked by viruses, but block most antiretroviral drugs access to the brain. What is the future? Nanoparticles?

Blood vessel arriving in the brain, surrounded by astrocytes

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Stephanie Huang @stephjhuang.bsky.social · 27/01/2025

A pyramidal neuron from the prefrontal cortex. #microscopymonday Labelled with DiI, imaged with the FV3000 CLSM, & depicted using @kwolbachia.bsky.social new KTZ_bw_kawa LUT.

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Elisa Galliano @elisagalliano.bsky.social · 24/01/2025

The most beautiful neurons in the brain (fact), and also my villain origin story - #FluorescenceFriday 🧡 biocytin patched and filled Purkinje cell 💚 parvalbumin 💜 AnkG for axon initial segments

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Melanie D. White @melaniedwhite.bsky.social · 24/01/2025

Happy #FluorescenceFriday to those who celebrate! Here's a beautiful quail neural tube and somites labelled for microtubules 🟠 and actin 🔵. Imaged by Power-RA Siew Zhuan Tan. #devbio 🧪🔬👩‍🔬

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Christophe 🔬 Leterrier @christlet.bsky.social · 10/01/2025

Spiny night for #FluorescenceFriday! Trying some out of left field ways to visualize busy samples. Actin (rainbow depth-color coded) and microtubules (gray) along mature dendrites. projection of a 3D-SIM stack with some undisclosed tricks

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Rachael Ott @rachaelott.bsky.social · 10/01/2025

Trying out new LUTs from @kwolbachia.bsky.social this #FluorescenceFriday, actin cytoskeleton is shown here in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy

The actin cytoskeleton (blue/green) is shown in a primary hippocampal neuron. The LUT used here is KTZ_bw_SeaWeed.

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Danielle Beckman @daniellebeckman.bsky.social · 10/01/2025

An interesting case of intense inflammatory response (astrogliosis) in the basal ganglia of a monkey infected with #SARSCov2. These astrocytes are giving no chance to this poor neuron. Markers: neuron (🟠SMI32), astrocytes (🔵GFAP), red blood cells (🔴RBC). Happy #FluorescenceFriday!

Micrograph showing several astrocytes interacting with a single neuron in an inflammatory response to SARS-COV-2 infection

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Stacey Ogden 🦔 @labogden.bsky.social · 09/01/2025

It's almost #FluorescenceFriday! Here is a ~E9.5 mouse neural tube section showing the signaling protein Sonic Hedgehog (SHH,green) and the SHH-specified floor plate (marked by FoxA2, magenta). We are interested in how SHH travels from signal-producing to signal-receiving cells. 🔬 Miriam Dillard 🧪 👩‍🔬

Micrograph of a section of a developing neural tube from an E9.5 mouse. SHH (green) is a signaling protein that instructs cell fate of cells of the neural tube. The magenta colored cells are expressing the neural tube floor plate marker FoxA2. Nuclei are marked by DAPI (blue). Image provided by Lab Manager Miriam Dillard.

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Danielle Beckman @daniellebeckman.bsky.social · 06/01/2025

Electric fence or radial glia? These cells🟡 are present only during 🧠development in the neocortex and guide immature neurons 🟣to their final position! Microglia/macrophages in 🔵. It was supposed to be a #FluorescenceFriday, now a #SynapticSunday, maybe #MicroscopyMonday, depending where you are! 🤓

Microscopy photo showing radial glia, neurons and macrophages in the neocortex

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Keri Martinowich @martinowk.bsky.social · 03/01/2025

Posting another image of these for #FluorescenceFriday at a different A-P axis level: interface islands in nucleus accumbens of human 🧠. I can't stop looking at these images as we get them - totally wild to see them in human brain - image credit to Svitlana Bach/Yufeng Du. 🔬👩‍🔬🧪

Cluster of interface island cells expressing OPRM1, RXFP1 and DRD1 in human brain

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Danielle Beckman @daniellebeckman.bsky.social · 27/12/2024

2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)

Big blue neurons surrounded by small yellow microglia everywhere!

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