Lee et al. deplete the cohesin component RAD21 in mouse embryonic stem cells. Most but not all chromatin loops fail to reform after mitosis, and transcriptional changes were minor, except during diffe...

Mouse embryonic stem cells (mESCs), in addition to differentiating into the three germ layers, can reverse typical developmental trajectories, as exemplified by their ability to de-differentiate into ...

Pluripotent stem cells are susceptible to acquiring detrimental epigenetic abnormalities, posing a hurdle to their safe use in biomedical applications. Using pluripotent cells with defined genetic bac...