Simulations integrating micro-C and imaging data provide a coherent view of chromatin organization from nucleosomes to clutches to domains, revealing that most regions form compact domains but short r...

A photolabile oligonucleotide system enables spatially resolved single-cell measurements to show how the epigenome regulates gene expression.

Researchers show that the zoonotic parasite Toxoplasma gondii built a unique chromatin modifying complex to activate the genes that drive infection and virulence, revealing a potential weak spot for n...

Ji et al. show that radiotherapy induces a TET2-dependent epitranscriptomic program in myeloid cells that promotes immunosuppressive M-MDSC differentiation. TET2 loss preserves caRNA m5C-MBD6 signaling, restores IRF3-IFN-I activation, generates myeloid-derived activating cells, and enhances radiotherapy-induced antitumor immunity.

In this Review, Buckley et al. discuss how DNA methylation is interpreted in mammals and plants, focusing on the reader proteins and transcription factors that bind to methylated DNA and the downstrea...

Viral mimicry is the activation of antiviral immune responses by endogenous nucleic acids that have accumulated in the cytosol. In this Review, Chen et al. highlight how cancer-associated epigene...

The Rad6B–Tex1 ubiquitination complex drives malaria parasite sexual commitment by monoubiquitinating histone H2B, and thereby enabling gametocyte formation and mosquito transmission.

The authors identified and engineered a miniature, innate nuclease-dead xCas12m enabling effective epigenome editing. xCas12m–CRISPRoff achieved durable epigenetic silencing and inhibition of hepatitis B virus infection through single-AAV delivery.

Single-molecule genomics methods are used to study the activity of regulatory factors on individual DNA molecules genome-wide, thereby enabling quantification of molecular heterogeneity and co-occurre...

CD8+ T cell dysfunction is a major obstacle to hepatitis B virus (HBV) clearance and antitumor immunity. Zhou and colleagues identify a T cell receptor targeting a clinically relevant HBV epitope and ...

Xie, Tan, Mo and colleagues show that a liver-specific bipartite structure of the active X chromosome is lost early in hepatocarcinogenesis in mice and humans, driving X-linked gene activation and premalignant transformation, particularly in males.

The genomic regions regulating gene expression are often themselves transcribed into a variety of noncoding RNAs (ncRNAs). However, the regulatory roles of this noncoding transcription remain largely unknown. By using live imaging, we reveal that the ...

In this Review, Izzo et al. discuss how single-cell multimodal sequencing enables genotype–phenotype mapping and, when combined with phylogenetic reconstruction, provide insights into clonal evolution, cell state plasticity, and cancer progression, revealing mutant-specific vulnerabilities and offering a framework to study somatic evolution across pre-malignant and malignant human tissues.