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Bui Lab @McGill

@builab.bsky.social
215 followers 155 following 160 posts
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Bui Lab @McGill @builab.bsky.social · 16/09/2026
Our #cartwheel paper from #Trichonympha led by my grad student Lee is finally online at www.pnas.org/doi/10.1073/.... Big congratulations to all the people who contributed 🎉 Since we put a lot of effort into the covers but didn't get chosen, I'm putting them here so everyone can see them.
A sketch up by my daughterTrichonympha and the ringA delicious cartwheel cakecartwheel universe
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John Wallingford @jbwallingford.bsky.social · 04/08/2026
Cilia alert! Last year Juyeon Hong described a new domain in 9+2 cilia called the EDT, the extreme distal tip. Now, she's found a new protein required for its assembly, but only in non-mammalian vertebrates. A cool evo-cell / protein evolution story in @cellreports.bsky.social tinyurl.com/2xvdskx6
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Bui Lab @McGill @builab.bsky.social · 04/08/2026
It all started when undergrads Tina & Helena set out to solve the structure of SPEF1 on microtubule seams. Designing for users with zero command-line experience pushed us to make CsMT as easy as possible: just import our JSON file into CryoSPARC, and all parameters are preloaded!
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Bui Lab @McGill @builab.bsky.social · 04/08/2026
Introducing CsMT: A new CryoSPARC workflow for high-resolution microtubule reconstruction based on novel PF-pair classification! You can reconstruct MTs entirely in CryoSPARC—zero custom scripts needed. Inspired & built through a fantastic collaboration with the Cianfrocco lab! tinyurl.com/kdpm86tw
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Bui Lab @McGill @builab.bsky.social · 04/08/2026
It all started when two undergrads Tina & Helena set out to solve the structure of SPEF1 on microtubule seams. Designing for users with zero command-line experience pushed us to make CsMT as easy as possible: just import our JSON into CryoSPARC, and all parameters are preloaded!
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Mart So-Last @mgflast.bsky.social · 31/07/2026
Made a little plugin for cryoEM map subboxing in ChimeraX: github.com/mgflast/Chim...
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EuroCilia2027 @eurocilia.bsky.social · 24/07/2026
📣 Abstract submission and early registration are now OPEN for the 7th European Cilia Conference—an official EMBO workshop! 📅 8–12 March 2027 📍 Stresa, Italy Submit and register: meetings.embo.org/event/27-cilia www.eurocilia.eu #EMBOCilia #Cilia2027
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Bui Lab @McGill @builab.bsky.social · 23/07/2026
Exciting new work from Elaine Wang, PhD student co-supervised with Natalie Zeytuni! Check out their latest structural insights into native Type V fimbriae from Porphyromonas gingivalis: www.biorxiv.org/content/10.6...
biorxiv.org
Structural tuning of native type V fimbriae shapes mechanical specialization in Porphyromonas gingivalis
Bacterial fimbriae are central to adhesion, colonization, and biofilm formation, yet how related fimbriae systems are structurally and mechanically specialized within the same pathogen remains poorly ...
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Bui Lab @McGill @builab.bsky.social · 20/07/2026
I used to have a lot of 3d printer problems in the distant past. Last year, I bought the Bambu Lab P1S, and it is surprisingly easy to work.
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Bui Lab @McGill @builab.bsky.social · 20/07/2026
Interested in 3D-printed models of cryo-EM grids, autogrid and cryo-FIB for visual teaching/decoration. Download our model and print: makerworld.com/en/models/30... Designed in Blender by Khan Bao, an undergrad in the lab.
makerworld.com
Cryo-EM Grids for Teaching - Free 3D Print Model - MakerWorld
Download this free 3D print file designed by CiliaBuilder. EM Grid Scaled ModelScaled up and simplified 3D model of EM grids and autogrids for demonstration of preparation process and milling. Content...
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Utz Ermel @uermel.bsky.social · 20/04/2026
Excited to share our paper about copick, a dataset API and toolkit for collaborative annotation and analysis of #cryoET data! Whether you're picking particles or curating segmentations, copick reduces friction and brings #OME-Zarr to cryoET without breaking pipelines. 🧵👇 doi.org/10.1002/pro.70578
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Bui Lab @McGill @builab.bsky.social · 13/04/2026
Is the alt text AI generated? It is trolling Jurgen.
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Bui Lab @McGill @builab.bsky.social · 12/04/2026
Oops, I forgot the preprint link www.biorxiv.org/content/10.6...
biorxiv.org
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Bui Lab @McGill @builab.bsky.social · 12/04/2026
Check out @lee in action, harvesting hindguts from termites! 🐜🔬. Massive credit for pionering work from @centriolelab.bsky.social and others for establishing the isolation of centriole from Trichonympha and earlier model for central hub. We significantly benefit from advances in cryo-ET & STA.
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Bui Lab @McGill @builab.bsky.social · 12/04/2026
The cartwheel was first visualized ~70 years ago in the symbiont Trichonympha in the wood termite gut. We’ve discovered the cartwheel’s secret: a unique zigzag stacking of SAS-6 tetramers that facilitates the iconic 9-fold symmetry of cilia. #cellbiology #teamtomo #SAS6 #cartwheel
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Bui Lab @McGill @builab.bsky.social · 27/02/2026
I see. I just have a quick look through the figure. I can try it to see then.
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Bui Lab @McGill @builab.bsky.social · 27/02/2026
Late to the thread,I wonder the difference to DeepDeWedge is due to CTF. When I used CTF corrected tomo to compare DeepDeWedge and IsoNet2, I saw no noticeable visual difference in my tomogram.
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Bui Lab @McGill @builab.bsky.social · 21/02/2026
That looks sick :)
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Bui Lab @McGill @builab.bsky.social · 21/02/2026
I used Tinkercad. Lots of tutorials on YouTube and it has very simple interface. The dimension I guessed a bit. A few trials and errors on print direction, design the join and groove.
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Bui Lab @McGill @builab.bsky.social · 21/02/2026
The printing profile & the Stl file can be downloaded here: makerworld.com/en/models/24... You need an AMS to print with color or just a single black for everything.
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Bui Lab @McGill @builab.bsky.social · 21/02/2026
3D printing is way too much fun 😄 Just made myself a Titan Krios G5 pen holder 🧊🔬 Now the only thing missing… is McGill getting the real one! 😉
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Bui Lab @McGill @builab.bsky.social · 06/02/2026
I also started a MakerWorld page to share these files! There are only a few for now, but I’ll be uploading more as I go further down the 3D printing rabbit hole. 🕳️🐇 Grab the models here: makerworld.com/en/@user_763...
makerworld.com
CiliaBuilder | Home - MakerWorld
MakerWorld is the leading 3D printing model community for designers and makers. Download thousands of free 3D models and STL files from the No.1 destination for multicolor designs.
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Bui Lab @McGill @builab.bsky.social · 06/02/2026
Ever wanted to hold a cilium in your hand? 🧬✨ Here is a look at how I’m using CiliaBuilder in @UCSFChimeraX to generate accurate 3D prints of centrioles and cilia. Perfect for teaching or lab displays! Watch the process: youtu.be/IKnnp3ovptQ #CryoEM #CellBio #ChimeraX
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Helen Foster @helenfoster.bsky.social · 06/02/2026
Very happy to say that our work on how axonemes are generated is now available on bioRxiv: www.biorxiv.org/content/10.6... - if you’ve ever wondered how the incredibly beautiful, complex structures within motile #cilia are constructed then this is for you!
biorxiv.org
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Bui Lab @McGill @builab.bsky.social · 04/02/2026
I haven't tried the new version, but I followed the tutorial and no membrane thickness is measured despite the fact that other properties are measured, which is one of the main thing that I am interested
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John Wallingford @jbwallingford.bsky.social · 29/01/2026
Cilia alert! Stoked to have the new paper from Juyeon Hong about a new domain at the extreme distal tip of motile cilia! (The EDT, y'all!) It's out now @natcomms.nature.com. Check it out! #cilia www.nature.com/articles/s41...
nature.com
A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function - Nature Communications
Here they combine in vivo imaging with proteomics to characterize a protein complex comprised of Ccdc78 and Ccdc33 that acts at the extreme distal tip of 9 + 2 motile cilia to control tip architecture...
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Bui Lab @McGill @builab.bsky.social · 28/12/2025
The entire video list with Installation, Build Cilia, Centriole & 2D template www.youtube.com/playlist?lis...
youtube.com
CiliaBuilder Tutorials - YouTube
CiliaBuilder Tutorials
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Bui Lab @McGill @builab.bsky.social · 28/12/2025
CiliaBuilder Video Tutorial www.youtube.com/watch?v=SJHo... If your installation didn't work a few days ago, try again. I've fixed a few things, and let me know if it still doesn't work for you.
youtube.com
ChimeraX CiliaBuilder - Modelling Cilia Basics
YouTube video by Bui Lab
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Bui Lab @McGill @builab.bsky.social · 24/12/2025
I just noticed that I did not put the proper dependency. Now, if you download version 1.0.6, it will install the dependency properly to run.
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Bui Lab @McGill @builab.bsky.social · 23/12/2025
I am planning to do the next release with ArtiaX style cilia in the near future :) You probably will like that a lot more.
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Bui Lab @McGill @builab.bsky.social · 23/12/2025
This was built in ~10 days of vibe-coding with Claude & Gemini. Now officially on holiday 😌 Video tutorial coming after the holidays
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Bui Lab @McGill @builab.bsky.social · 23/12/2025
Tired of drawing cilia or centrioles for slides and papers? I feel you. Check out CiliaBuilder for ChimeraX → cxtoolshed.rbvi.ucsf.edu/apps/chimera... Build custom 3D cilia & centriole models (tips + primary cilia included).
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Bui Lab @McGill @builab.bsky.social · 11/07/2025
We also analysed SPEF1-KO cilia by cryo-ET to show that SPEF1 restricts C1 and C2 microtubule movement.
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Bui Lab @McGill @builab.bsky.social · 11/07/2025
In the final version, we included a lot more data and analysis compared to the previous biorxiv version. We achieved a 4.6 Angstrom resolution of the microtubule by subtomogram averaging of MT-SPEF1 complexes.
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Bui Lab @McGill @builab.bsky.social · 11/07/2025
Our newest paper is now online in Current Biology. In short, we identified 7 proteins in the tip of the cilia and found SPEF1 is a seam-binding protein and important for cilia stability. Great work from my postdoc Thibault Legal and everyone involved! www.sciencedirect.com/science/arti...
sciencedirect.com
Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1
Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The cil…
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Bui Lab @McGill @builab.bsky.social · 08/03/2025
This seems great. I want to working on our picking using AI in the summer. Perhaps, I can ask you for an installation and usage guide if I have problems.
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Bui Lab @McGill @builab.bsky.social · 06/03/2025
I hated Amira with the $$. Need to try DragonFly but most of our processing is done on the cluster so anything using command line is a big advantage. Perhaps, my summer project :)
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Bui Lab @McGill @builab.bsky.social · 05/03/2025
interesting I want to use dragonfly for segmentation but can we run dragonfly in command line for batch mode? Do you have a script to cluster and turn dragon fly into filament light?
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Bui Lab @McGill @builab.bsky.social · 05/03/2025
Very nice preprint from the Wallingford lab. Seems like there is a common theme of tip proteins as these proteins seem to be in the tip of the ciliate cilia too.
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Bui Lab @McGill @builab.bsky.social · 05/03/2025
Thanks. I found template matching is too slow for my need as I can manual pick microtubules much faster and more accurate. These tryout takes time so I 'd love to see people's experiences.
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Bui Lab @McGill @builab.bsky.social · 26/02/2025
#TeamTomo What is the open-source and good cryoET deep-picking program?
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The EMBO Journal @embojournal.org · 24/02/2025
Cryo-electron tomography of Tetrahymena #cilia by Sam Li, David Agard, Mark Winey provides insights into the intricate spatial organization of basal body inner junctions, compromised by loss of Poc1 www.embopress.org/doi/full/10....
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Yoshi Ichikawa @ichikawa-lab.bsky.social · 28/01/2025
We have posted our new #preprint regarding the track recognition mechanism of dynein-2 that works in #cilia on bioRxiv @biorxivpreprint.bsky.social!! www.biorxiv.org/content/10.1... 1/8 🧵
biorxiv.org
Dynein-2 is tuned for the A-tubule of the ciliary doublet through tubulin tyrosination
Eukaryotic cilia and flagella are thin structures present on the surface of cells, playing vital roles in signaling and cellular motion. Cilia structures rely on intraflagellar transport (IFT), which ...
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Pengxin Chai @pengxinchai.bsky.social · 21/01/2025
Excited to share our work on bioRxiv before my thesis defense! Together with my undergraduate mentee Diego, postdocs Wan and Jun, and my advisor Kai, we identified a novel dynein heavy chain subfamily, DNAHX, from sea urchin sperm axoneme. Check out the details here: www.biorxiv.org/content/10.1...
biorxiv.org
DNAHX: a novel, non-motile dynein heavy chain subfamily, identified by cryo-EM endogenously
Ciliogenesis and cilia motility rely on the coordinated actions of diverse dyneins, yet the complexity of these motor proteins in cilia has posed challenges for understanding their specific roles. Tra...
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Rui Zhang @ruizhangmt.bsky.social · 01/01/2025
Best way to start 2025! Thrilled to share our Nature paper about the structures of axonemal components from sperm flagella and from epithelial cilia of the oviduct and brain ventricles. Great collaboration with Tzviya Zeev-Ben-Mordehai and @alanbrownhms.bsky.social www.nature.com/articles/s41...
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Bui Lab @McGill @builab.bsky.social · 17/12/2024
Amazing work. It is so useful for us working with Tetrahymena and for cilia in general.
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Caitie McCafferty @computingcaitie.bsky.social · 16/12/2024
I am excited to share some of my PhD work on the motile cilia interactome revealed by XL/MS with @jbwallingford.bsky.social @edwardmarcotte.bsky.social Ophelia Papoulas, Chanjae Lee, David Taylor, and @builab.bsky.social www.cell.com/developmenta...
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ChimeraX @chimerax.ucsf.edu · 13/12/2024
ChimeraX 1.9 has been released and is available now on our website
cgl.ucsf.edu
Download UCSF ChimeraX
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Bui Lab @McGill @builab.bsky.social · 05/12/2024
Thanks. Warp on Linux is awesome. This is my Twitter import so it is very old tweet
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Bui Lab @McGill @builab.bsky.social · 04/12/2024
My last post on Twitter before.
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