iiinova — where your digital self keeps evolving

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Ellenberg Lab @ellenberglab.bsky.social · 10/09/2025

Our work on NPC biogenesis is now out! Wanlu Zhang investigated the role of FG-Nups in NPC reassembly, pore dilation, and the nuclear envelope remodelling using acute depletions, correlative EM, and MINFLUX imaging 💫. Thanks, Wanlu and all the collaborators!

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Andi Brunner @andibrunner.bsky.social · 10/09/2025

Congratulations Wanlu!! 🚀🚀

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Jeremy Cliffe @jeremycliffe.bsky.social · 24/08/2025

Just got round to watching Mario Draghi's speech in Rimini in the original Italian. It is easily the most important speech on Europe so far this year and I hope someone (@grandcontinent.bsky.social?‬) publishes it in English and other EU languages soon. www.youtube.com/watch?v=YMME...

youtube.com

Mario Draghi’s Full Speech: Europe’s Harsh Reality — Powerless on the Global Stage! -ITALIAN-

YouTube video by EU Debates | eudebates.tv

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Andi Brunner @andibrunner.bsky.social · 21/07/2025

Indeed as Ilya says, BrdU should only be added for a single S-phase. The longer you incubate with BrdU (the more replication cycles incorporate BrdU) the less available DNA you will habe after the RASER-FISH procedure :)

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Ellenberg Lab @ellenberglab.bsky.social · 21/07/2025

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out! www.nature.com/articles/s41... Congratulations to Kai and Oyvind!!

nature.com

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications

A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

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Andi Brunner @andibrunner.bsky.social · 21/07/2025

Indeed difficult! Only the replicated strands are available for degradation, do ideal would be to BrdU-label in the S-phase prior to fixation. I guess for zebrafish embryos there is no uptake from outside?

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Andi Brunner @andibrunner.bsky.social · 20/07/2025

BrdU incorporation during S-phase, UV-induced single-strand nicking after cell fixation and Exonuclease based digestion of the nicked DNA strand! :)

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Claas Gefroi @claasgefroi.bsky.social · 10/05/2025

"Nicht einen Millimeter überlassen..." Ich habe das jetzt erst gesehen und was soll ich sagen, was Igor Levit da anlässlich des Todes von Margot Friedländer aus dem Stand heraus sagt, ist das Berührendste, Wahrste und Richtigste, was ich seit ewig im deutschen Fernsehen gehört habe.

youtube.com

Igor Levit über Margot Friedländer beim Deutschen Filmpreis 2025 -

YouTube video by Rederei FM

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Laura Rustarazo-Calvo @laura-rustarazo.bsky.social · 27/03/2025

🎉 Excited to share our new work: “Adhesion-driven tissue rigidification triggers epithelial cell polarity”, now on @biorxivpreprint.bsky.social ! A huge thank you to @nicolettapetridou.bsky.social, Bernat, @crisp-c.bsky.social, Adrián, and everyone involved! 🙌 🧵⤵️

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Deutscher Wetterdienst (DWD) @dwdderwetterdienst.bsky.social · 15/04/2025

DWD-Fakten zur aktuellen #Trockenheit in #Deutschland: Noch nie seit Beginn der Auswertung 1931 war es in Deutschland im Zeitraum von Anfang Februar bis Mitte April so trocken wie 2025. Mehr Details auch zu #Europa in der heutigen Pressemitteilung unter www.dwd.de/presse

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John Scalzi @scalzi.com · 09/04/2025

Spoiler: It is absolutely market manipulation

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EMBL @embl.org · 24/03/2025

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪 New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation. @ellenberglab.bsky.social @andibrunner.bsky.social www.embl.org/news/science...

embl.org

How chromosomes shape up for cell division | EMBL

EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

This has been an excellent collaboration together with Kai Beckwith in the @ellenberglab.bsky.social @embl.org. Thanks a lot to Kai, Jan, Natalia, and our collaborator Ralf Jungmann!! A summary of the work can also be read at the @embl.org press release: www.embl.org/news/science...

embl.org

How chromosomes shape up for cell division | EMBL

EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

We thus propose that mitotic chromosome shape is dictated by Condensin-driven extrusion of long and overlapping loops up to a density set by chromatin self-repulsion.

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

Feature 1: Condensins pass each other. This leads to the formation of large, extensively overlapping loops with sizes up to 6-8 Mb. Feature 2: DNA self-repulsion. This leads to self-organization and stacking of loops into the rod-shape.

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

Using a dynamic polymer constrained only by available quantitative Condensin chromatin-binding data, we could recapitulate the formation of native mitotic chromosome rods! Two features were essential for that ->

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

We found that Condensins are also responsible for the backfolding of DNA at much larger genomic distances: up to 6-8 megabases.

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

Analyzing these traces, we found evidence for continuously growing loops, and our degron-based experiments showed that these loops are built by the Condensin complexes.

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

Kai and I used the recently established LoopTrace technology to trace the structure of single mitotic chromosomes in human cells in a very mild & structure pre-serving manner.

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Andi Brunner @andibrunner.bsky.social · 24/03/2025

Mitotic chromosomes form through long, overlapping and self-repelling DNA loops, generated by the Condensin complexes! Check out our newest paper here, and see below for a short Bluetorial! www.cell.com/cell/fulltex...

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Ellenberg Lab @ellenberglab.bsky.social · 19/03/2025

We are #hiring! We have two open postdoc/research scientist positions to join our #ERC funded project MITOFOLD to resolve the organization of mitotic chromosomes! 1) experimental biologist shorturl.at/LjGEM 2) data scientist shorturl.at/nDdhQ Please share! 🙌 We look forward to your application! 🤩

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Liz Cheney @lizcheney.bsky.social · 28/02/2025

History will remember this day— when an American President and Vice President abandoned all we stand for.

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Daniel Drepper @danieldrepper.bsky.social · 25/02/2025

Zwei Tage vor der der Wahl haben Friedrich Merz und die Union eine Anfrage gestellt, die man in dieser Form sonst nur von der AfD kannte. In 551 (!) Fragen stellen sie die Gemeinnützigkeit zahlreicher NGOs in Frage, darunter auch des @netzwerkrecherche.org. dserver.bundestag.de/btd/20/150/2...

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Prof. Stefan Rahmstorf @rahmstorf.bsky.social · 22/02/2025

🇩🇪 ist die drittgrößte Volkswirtschaft der Welt. Morgen wählen wir - entweder für eine moderne, klimaneutrale Wirtschaft, oder für die Rückkehr zu Technologien des letzten Jahrhunderts. Lasst euch nicht für dumm verkaufen mit Scheinlösungen, die nur das fossile Geschäftsmodell noch etwas verlängern.

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Andi Brunner @andibrunner.bsky.social · 19/02/2025

In today‘s talk on „Autocracy vs Democracy“ in Heidelberg, Prof. Dr. Aurel Croissant said that 7/9 landmark developments towards #autocracy have been made in the US. A change back to more #democracy becomes increasingly unlikely. At the same time, the white house posted this on X, citing #Trump.

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Robert Habeck @robert-habeck.de · 10/02/2025

Ich rechne damit, dass heute, Vorwürfe gegen meine Doktorarbeit, die ich vor 25 Jahren geschrieben habe, veröffentlicht werden. Ich habe mich entschieden, das Ganze transparent zu machen. Denn ich kenne die Vorwürfe – und konnte sie vorab prüfen lassen. www.youtube.com/watch?v=mYyA...

youtube.com

Zu meiner Dissertation | Robert Habeck

YouTube video by Robert Habeck

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Antonia Juhasz @antoniajuhasz.bsky.social · 08/02/2025

Erasure. At the National Institute of Health, posters of women and scientists of color have been taken down. Shared by @ivancalcantara.bsky.social

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Duncan Allen @duncanallen.bsky.social · 07/02/2025

Doing a report on AfD campaigning on Facebook and it really is striking the sheer level of AI images and videos. Smiling white (often blonde) Germans contrasted against glowering dark skinned men. Shameless stuff, but perhaps I shouldn't be surprised

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Ellenberg Lab @ellenberglab.bsky.social · 09/01/2025

Congratulations to @andibrunner.bsky.social and the team, and many thanks to the lab of Jan-Michael Peters for support! We found that the interphase and mitotic chromatin loop organization have more in common than previously thought: In both stages big loops are built first, small ones second

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Andi Brunner @andibrunner.bsky.social · 09/01/2025

My first own project is published: Despite major differences in chromosome shape during #interphase and #mitosis, the underlying #loop-extruder based organization is highly similar A new data resource and new insights into human chromatin biology! 🧬🥳

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Journal of Cell Biology @jcb.org · 09/01/2025

@andibrunner.bsky.social et al. @ellenberglab.bsky.social show that interphase organization into Cohesin-driven loops occurs in a sequential and hierarchical manner - just like the mitotic organization by Condensins. buff.ly/426gIEu @wanluzhang.bsky.social

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Ellenberg Lab @ellenberglab.bsky.social · 25/11/2024

We are now on Bluesky! Follow us for exciting #Science around #CellDivision and nuclear organization, illuminated using advanced #imaging #technology - like our last preprint on mitotic chromosome structure and organization: tinyurl.com/yaznnkku

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