iiinova — where your digital self keeps evolving

Reposted by Tomas Pachano

Mikko Taipale @miketilapia.bsky.social · 30/06/2026

Our evil ORFeome paper is out! In collaboration with @alex-stark.bsky.social, we screened ~4,000 viral proteins and secreted effectors from bacteria and parasites, covering hundreds of diverse pathogens, for phenotypes in human cells.

29943

Tomas Pachano @tomaspachano.bsky.social · 30/06/2026

This was a major collaborative effort with He Leng and the teams at the @alex-stark.bsky.social Lab (@impvienna.bsky.social ) and the @miketilapia.bsky.social Lab (@donnellycentre.bsky.social). (4/4)

070

Tomas Pachano @tomaspachano.bsky.social · 30/06/2026

To demonstrate the platform's discovery power, we characterized: •HHV6A U14 as a potent p53 antagonist that drives tumorigenesis in vivo •HHV7 U21 as a dual-function effector blocking STING signaling and MHC-I display •Adenoviral 13.6K as a recently evolved TAP inhibitor (3/4)

130

Tomas Pachano @tomaspachano.bsky.social · 30/06/2026

Here, we developed the eORFeome, a functional genomics platform to systematically map how pathogens hijack human cells. By screening 4,000 effectors from diverse viruses, bacteria, and parasites, we identified novel pathway-modulating functions for hundreds of proteins. (2/4)

120

Tomas Pachano @tomaspachano.bsky.social · 30/06/2026

This project was a major collaborative effort between the Stark Lab (IMP, Vienna) and the Taipale Lab (Donnelly Centre, Toronto). (4/4)

000

Tomas Pachano @tomaspachano.bsky.social · 30/06/2026

To demonstrate the platform's discovery power, we characterized: • HHV6A U14 as a potent p53 antagonist that drives tumorigenesis in vivo. • HHV7 U21 as a dual-function effector blocking STING signaling and MHC-I display. • Adenoviral 13.6K as a recently evolved TAP inhibitor. (3/4)

100

Tomas Pachano @tomaspachano.bsky.social · 30/06/2026

In this work, we developed the eORFeome, a functional genomics platform to systematically map how pathogens hijack human cells. By screening nearly 4,000 effectors from diverse viruses, bacteria, and parasites, we identified novel pathway-modulating functions for hundreds of proteins. (2/4)

100

Reposted by Tomas Pachano

IMP @impvienna.bsky.social · 30/06/2026

Pathogens have spent millions of years learning how to hijack human cells. Now, researchers from the Stark lab at the IMP and the Taipale lab at the Donnelly Centre, University of Toronto, have built a platform to systematically discover how they do it:

1239