iiinova — where your digital self keeps evolving

Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

A BIG thank you to everyone involved in this project throughout the years: @wimderave.bsky.social, @debocklab.bsky.social, Erik Richter, @jvdesomp.bsky.social, @maxullrich.bsky.social and everyone not active on this platform 🤩 (11/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

All this and more in the full version of our paper: sciencedirect.com/science/arti... (10/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

Instead, we believe this is mainly caused by a blunted inflammatory response from blocking H1 receptors in the muscle microenvironment and a lowered glucose supply. (9/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

We then blocked this pathway via administration of H1 and H2 receptor antagonists to humans during and following exercise. Main observation: blocking H1 receptors resulted in an impaired glycogen resynthesis after exercise, independent of canonical insulin signaling. (8/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

Is this pathway activated by exercise? Yes! We observed a very clear increase in histamine concentration in the muscle interstitial fluid during exercise. And this is a very local phenomenon in the muscle microenvironment, as we did not see a spillover in the circulation (7/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

Histamine likely signals by being produced in mast cells (via the HDC enzyme) and binding to the specific histamine H1 and H2 receptors that are mainly expressed on myeloid and some vascular cells. (6/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

By performing some bioinformatic modeling of cellular crosstalk, focused on these mast cells, we obtained new insights into potential signaling networks driven by mast cells. One pathway sparked our interest: histamine signaling from mast cells to myeloid cells (5/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

This suggested to us that the mononuclear non-muscle cells in skeletal muscle might play a crucial role. We composed a complete cellular profile of skeletal muscle by integrating a single-cell and single-fiber RNA-seq dataset. Look at the small mast cell population! (4/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

To start this off, we performed transcriptional profiling of exercise effects in bulk muscle (containing many non-muscle cells) versus isolated muscle fibers. Vast effects in bulk muscle were observed (as expected), but this was much more modest in the muscle fibers (3/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

We previously showed that oral intake of histamine receptor antagonists severely blunts the positive health effects of 6 weeks of exercise training (pubmed.ncbi.nlm.nih.gov/33853781/). We now wanted to take a deep dive into the molecular mechanisms 🧬 (2/11)

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 07/02/2025

Cellular crosstalk in skeletal muscle? A surprising role for mast cells? Histamine as a signal transducer during exercise? Check out the story in @cp-cellmetabolism.bsky.social 🧵 @wimderave.bsky.social, @ugent-fge.bsky.social, @fwovlaanderen.bsky.social www.sciencedirect.com/science/arti...

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 20/11/2024

Could I be added as well? Thanks for this initiative.

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 20/11/2024

Would love to join as well. Really nice initiative!

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Reposted by Thibaux Van der Stede

Katie Whytock @kwhytock.bsky.social · 19/11/2024

More evidence of fiber type heterogeneity beyond the typical myosin heavy chains!

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Thibaux Van der Stede @thibauxvanderstede.bsky.social · 18/11/2024

Let’s try out this new platform by sharing our recent paper on the transcriptional signatures of individual muscle fibers following high-intensity exercise #myoblue #myosky journals.physiology.org/doi/abs/10.1...

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Reposted by Thibaux Van der Stede

Altmetric @altmetric.com · 18/11/2024

We strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics

docs.bsky.app

Action Intent Links | Bluesky

Authors, websites, and apps can use action intent links to implement "Share on Bluesky" buttons, or similar in-app actions. Logged-in users will be directed to the corresponding action view in the Blu...

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