iiinova — where your digital self keeps evolving

Tal Sharf @talsharf.bsky.social · 05/05/2026

This work has transformed how we analyze data, plan experiments, and conduct high-density electrophysiology studies in the lab. We hope these tools will be useful to others interested in analyzing complex datasets from high-density neurophysiology recordings.

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Tal Sharf @talsharf.bsky.social · 05/05/2026

New work from the lab is up on bioRxiv, where we introduce “SpikeLab: Agentic tools for spike data analysis” was led by postdoc Tjitse van der Molen and supported the crew at @ucscgenomics.bsky.social and @baskinengineering.bsky.social www.biorxiv.org/content/10.6...

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Reposted by Tal Sharf

Matteo Carandini @carandinilab.net · 22/12/2025

Sequences are everywhere! In every brain region. And are written in stone. Invariant Activity Sequences Across the Mouse Brain. Out today, by Célian Bimbard, with @kenneth-harris.bsky.social. Based on data by Célian and by @intlbrainlab.bsky.social. www.biorxiv.org/content/10.6...

Examples of sequences of activity seen in multiple regions of the brain

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Reposted by Tal Sharf

Kevin Mitchell @wiringthebrain.bsky.social · 25/11/2025

Slate status: not blank

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Tal Sharf @talsharf.bsky.social · 25/11/2025

Many thanks to Tjitse van der Molen, Alex Spaeth, @mattiachini.bsky.social and all the amazing collaborators who made this work possible.

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Tal Sharf @talsharf.bsky.social · 25/11/2025

Our findings support the idea that preconfigured temporal structure is an intrinsic, emergent property of neurodevelopment that is established even before sensory input.

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Tal Sharf @talsharf.bsky.social · 25/11/2025

Using high-density, large-scale neurophysiology recordings from organoids, we model early dynamics of early human brain development. We complement these data with matching neonatal mouse cortical recordings to examine the temporal firing patterns that emerge during early circuit assembly.

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Tal Sharf @talsharf.bsky.social · 25/11/2025

I was told it would take about three years to get a new lab up and running. Nearly three years later, our work on preconfigured neuronal firing sequences in human brain organoids is finally out in Nature Neuroscience: www.nature.com/articles/s41...

nature.com

Preconfigured neuronal firing sequences in human brain organoids - Nature Neuroscience

Examining human brain organoids and ex vivo neonatal murine cortical slices demonstrates that structured neuronal sequences emerge independently of sensory input, highlighting the potential of brain o...

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Tal Sharf @talsharf.bsky.social · 31/10/2025

"We can, should, and will use cerebral organoids to discover new medical treatments, study brain development, reduce the demand for animal testing, and even power computers and more energy-efficient AI." www.vox.com/future-perfe...

vox.com

The challenge of creating brains in a lab

Lab-grown brains don’t have to inspire horror.

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Reposted by Tal Sharf

Emily R. Trunnell, Ph.D. @ertrunnell.bsky.social · 28/10/2025

In an NSF-supported study, @talsharf.bsky.social and collaborators will investigate the capacity of human brain organoids to learn and adapt, with implications for #organoid research, #bioethics, and science education. news.ucsc.edu/2025/10/lear...

news.ucsc.edu

Effort aims to uncover the learning and reasoning potential of brain organoids

The Braingeneers team will to test the ability of brain organoids to solve tasks in real time

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Reposted by Tal Sharf

Carsen Stringer @computingnature.bsky.social · 12/01/2025

What if… spontaneous neural activity 🧠 reflects the baseline rumblings of a brainwide dynamical system initialized for learning? We find that the rumblings have macroscopic properties like those emerging from linear symmetric, critical systems 🧵 #neuroscience #neuroAI www.biorxiv.org/content/10.1...

schematic of neural recordings from mouse V1, whole-brain, and hippocampus; neural activity traces from the population, showing more correlated activity in V1 and whole-brain recordings versus more decorrelated activity in hippocampus

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Reposted by Tal Sharf

Yoshiho Ikeuchi @yoshihoikeuchi.bsky.social · 17/01/2025

Our manuscript preprint is out!

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Tal Sharf @talsharf.bsky.social · 09/01/2025

Our work demonstrates that the emergence of intrinsic computational architectures, defined by low- and high-dimensional activity patterns and neuronal sequences, is an emergent property of neuronal self-assembly and achievable in brain organoid models.

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Tal Sharf @talsharf.bsky.social · 09/01/2025

Phenomena such as "preplay," pre-existing sequence motifs are thought to encode novel experiences and underlie episodic memory retrieval in the human cortex. These motifs have recently been shown to encode non-redundant information beyond latency and rate encoding.

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Tal Sharf @talsharf.bsky.social · 09/01/2025

The use of neuronal sequences to encode sensory input and motor output has been widely studied. However, whether these sequences arise innately through neurogenesis and synaptogenesis (nature) or emerge through sequential experience (nurture) remains unresolved.

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Tal Sharf @talsharf.bsky.social · 09/01/2025

This conclusion can be made most powerfully in a brain organoid that does not have the intimate developmental interaction with other organ systems and thus excludes an internal setting due to cardiac signals, for example, of brain rhythms.

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Tal Sharf @talsharf.bsky.social · 09/01/2025

Employing human and murine brain organoids alongside ex vivo neonatal murine brain slices, we demonstrate that intrinsic neuronal sequences, which delineate low- and high-dimensional latent subspaces for information encoding, emerge independently of sensory experience.

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Tal Sharf @talsharf.bsky.social · 09/01/2025

In our updated manuscript we present strong evidence supporting the pre-configured brain hypothesis. Separating the effects of experience from intrinsic network dynamics has historically been challenging due to experimental limitations.

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Tal Sharf @talsharf.bsky.social · 09/01/2025

I’m thrilled to share our revised work on neuronal sequences in brain #organoids. It has been an absolute joy to collaborate with an incredible team of talented experimental and computational neuroscientists brought together by this project. www.biorxiv.org/content/10.1...

biorxiv.org

Protosequences in brain organoids model intrinsic brain states

Neuronal firing sequences are thought to be the basic building blocks of neural coding and information broadcasting within the brain. However, when sequences emerge during neurodevelopment remains unk...

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