Reposted by Steven Fanara
Happy to share our newest preprint. We developed guidelines and analysis workflows for FLIM Imaging in plants. Multiplexing with spectrally overlapping fluorophores in plants is now possible. Have a look. Any feedback is more than welcome.
www.biorxiv.org/content/10.6...
![Fig. 2.Localization of RS2Z proteins in all vegetative and reproductive organs. Translational fusions are expressed in seed coat (A/a), embryo (B/b), hypocotyl (C/c), and cotyledons (D/d), 2-week-old root epidermis (E/e) including root hairs (F/f), leaf epidermis (G/g–I/i) including trichomes (G/g and H/h) and stomata (I/i), immature floral bud (including sepals, petals, stamens, and pistil) (J/j), mature gynoecium (valves, stigma, and style) (K/k), mature androecium (anther and filament) (L/l), pollen grains [(M/m and N/n), arrow], ovules (O/o), and funiculi [(O/o), arrow], petals (including veins) (P/p) and sepals (including veins) (Q/q). Red signals represent chlorophyll autofluorescence. At least three independent T3 homozygous lines were generated and analyzed, revealing similar fluorescence profiles.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:ggz22gcwkakll7efac23g33s/bafkreibhelpcpqlrctcub7vqjtoble6zkgrop2wiu4mekifnsqhay4pwoq)
