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Reposted by The Freeman Lab

shawnferguson.bsky.social @shawnferguson.bsky.social · 22/04/2026

How do lysosomes rapidly adapt to membrane tension to avoid bursting? In this article, @laylanassar.bsky.social and I highlight discoveries from Angela Kim, Spencer Freeman and colleagues who show a role for TMEM63A, a mechanosensitive cation channel in protecting lysosomes doi.org/10.1083/jcb....

doi.org

A pressure relief valve for lysosomes

Nassar and Ferguson discuss work from Kim et al. showing that TMEM63A protects lysosomes from rupture by acting as a pressure relief valve when membrane te

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The Freeman Lab @spencerfreeman.bsky.social · 14/05/2025

We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...

HeLa cells stably expressing a dox-inducible active TMEM16F-GFP (blue). Once expressed, lipid scrambling of the plasma membrane bilayer causes vesiculation, shown with Annexin V staining of the exposed PS (gold).

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Reposted by The Freeman Lab

Journal of Cell Biology @jcb.org · 02/04/2025

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2, say Jing Ze Wu, Sergio Grinstein, Spencer Freeman and colleagues @sickkidsto.bsky.social: rupress.org/jcb/article/... #Immunology #Membrane #lipid #lysosomes #osteopetrosis

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Reposted by The Freeman Lab

Journal of Cell Biology @jcb.org · 26/03/2025

Pemberton, Burke, Balla et al. report the generation & characterization of an engineered active fragment of PIKfyve and its use to generate extra PI(3,5)P2 in endomembrane compartments & test the function of PI(3,5)P2 #biosensors rupress.org/jcb/article/... #lipids #CellSignaling

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The Freeman Lab @spencerfreeman.bsky.social · 27/03/2025

New tools and regulators of lysosomal PI(3,5)P2! Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60

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Reposted by The Freeman Lab

Andres Hidalgo @drusqui.bsky.social · 19/03/2025

Excited to post for the first time to present our exciting new paper @Nature. The fruit of massive work by Tommaso Vicanolo, @alazozcan.bsky.social and Jackson Li, and our superb collaborators Laiguan and Stefan Uderhardt. True teamwork! Free link: rdcu.be/eeeA2 www.nature.com/articles/s41...

rdcu.be

Matrix-producing neutrophils populate and shield the skin

Nature - A population of neutrophils in the skin produces extracellular matrix, providing a defence strategy by reinforcing the barrier properties of the skin and helping to block the entry of...

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The Freeman Lab @spencerfreeman.bsky.social · 27/02/2025

We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....

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