iiinova — where your digital self keeps evolving

Phosphorothioates protect therapeutic ASOs and siRNAs from nucleases. It turns out archaea make them naturally, with a single stereochemistry and dedicated writer enzymes. New in Cell from Maman et al. Read the paper linked in the comments below. #RNASky

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RNAConnect @rnaconnect.bsky.social · 19/09/2026

@genomeweb.bsky.social reports on new research showing a widespread RT-qPCR artifact is inflating apparent knockdown efficiency across RNA-targeting CRISPR systems that rely on retroviral RTs. UltraMarathonRT® avoids it. www.genomeweb.com/technology/g...

genomeweb.com

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RNAConnect @rnaconnect.bsky.social · 16/09/2026

Long-read RNA-seq libraries that decay before reaching the 5' end? Dr. Paul Boutz (University of Rochester) explains why non-processive RTs are the bottleneck and how UltraMarathonRT’s ® intrinsic helicase activity fixed it for his lab. Read the full BioTechniques interview linked below.

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RNAConnect @rnaconnect.bsky.social · 10/09/2026

Bound guide #RNA can stop a conventional RT before it reaches your target region, inflating apparent #CRISPR knockdown in #RTqPCR. UltraMarathonRT's strand displacement activity means it can read straight through it — accurate KD/KO quantitation validated across Csm, PspCas13b & CasRx. 🔗⬇

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RNAConnect @rnaconnect.bsky.social · 01/09/2026

Congratulations to Leslie Watkins, Alan Zhu & senior author Bin Wu at Johns Hopkins on identifying a pervasive #RTqPCR artifact that inflates apparent knockdown across RNA-targeting #CRISPR systems (Csm, PspCas13b, CasRx). Rigorous work the CRISPR field needed. 🎉

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Reposted by RNAConnect

CarpenterLab @carpenterlab.bsky.social · 18/08/2026

We couldn't be more excited for the start of the 2026 UCSC RNA futures meeting. It's going to be a fun week!!#RNAFutures2026. share.google/4CYqqExEx69i...

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RNAConnect @rnaconnect.bsky.social · 18/08/2026

We are exhibiting at #RNAFutures this week! Stop by our booth, and Jason Underwood will talk through how to improve your RNA-seq by getting more genes and longer, more complete transcripts with #uMRT. #RNA #RNAseq #DRS #transcriptomics @jg-underwood.bsky.social

RNAConnect is exhibiting at RNA Futures this week at UCSC.

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RNAConnect @rnaconnect.bsky.social · 11/08/2026

Steady-state transcriptomics hides the synthesis and decay kinetics that shape gene expression. AIR-seq, a new method from Harvard's Churchman lab, marks newly synthesized #RNA directly in standard #RNAseq libraries, no chemical conversion or enrichment required. #RNASky #bulkRNA #scRNA 🗞️🔗⬇️

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RNAConnect @rnaconnect.bsky.social · 05/08/2026

To sequence a cell, you've always had to destroy it. POND-seq changes that. Secretory nanocages export RNA complexes into supernatant — cells stay alive, beacons of RNA keep broadcasting. Science fiction turned methodology. #RNA #RNASky #RNAseq #RBP #RNAbiology #Transcriptomics

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RNAConnect @rnaconnect.bsky.social · 29/07/2026

Publicly available resource: 93K+ chemical perturbations meets #transcriptomic profiling. Rare transcript patterns reveal cell states — including ferroptosis resistance — that smaller-scale approaches miss entirely. This is where the field is going. #RNASky #RNA #RNAseq #GeneExpression

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RNAConnect @rnaconnect.bsky.social · 24/07/2026

Nature Biomed Eng (July 7): non-capped, non-polyadenylated RNA oligonucleotides efficiently activate CD8+ T cells in vivo. No canonical mRNA features required for translation or immunogenicity. The field's model of how RNA is translated just got more complicated. #RNASky #RNA #ChemRNA

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RNAConnect @rnaconnect.bsky.social · 18/06/2026

RNA hydrolysis at elevated RT temps isn't a footnote. It's actively degrading your long transcripts during the reaction. New data at #RNATx '26 quantifies how much and shows what changes with an RT at 30°C. 3 modalities: SR, PacBio LR, and ONT DRS. The gains for transcripts ≥10kb are substantial.

RNAConnect to present scientific poster at RNATx 2026.

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RNAConnect @rnaconnect.bsky.social · 11/06/2026

uMRT® has peer-reviewed publications across 5 application areas on our website. Find the work most relevant to your research, from rare #transcriptprofiling to #RNAmods mapping, #shortread or #longread #RNAseq; access key research within one click! See🔗below! #lncRNA #UltraMarathonRT #RNASky

Read UltraMarathonRT publications from PNAS, Nature, JMB, and RNA.

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RNAConnect @rnaconnect.bsky.social · 05/06/2026

Dr. Graveley's lab used uMRT to tackle the most complex #alternativesplicing in any model organism, #Drosophila #DSCAM, with its thousands of possible isoforms, and #circRNAs accumulating with age. His talk is a masterclass in what LR #RNASeq can do when your RT doesn't give up. bit.ly/4tNiZ1T

Direct RNA Sequencing of Dscam1 using UltraMarathonRT by RNAConnect

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RNAConnect @rnaconnect.bsky.social · 29/05/2026

Long transcripts still get lost in #ONT direct RNA-seq without the right RT. Tonight at #RNA2026, we're sharing new data on how uMRT fixes that. (P2-293) Improved long transcript representation in ONT direct RNA-seq with UltraMarathonRT® — tonight, 8:30–10:30 PM, Room 517bc & 520 #RNA #RNASeq

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RNAConnect @rnaconnect.bsky.social · 27/05/2026

MMLVs stall on long, structured RNA, and the mischaracterizations of the transcriptome often go undetected in your data. We're presenting new data at #RNA2026 tonight! May 27, 8–10 PM (ID# 672) Ultra long transcript identification in long-read RNA-seq with uMRT 🔗 👇 @rnasociety.bsky.social #RNA

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RNAConnect @rnaconnect.bsky.social · 26/05/2026

If you're running #ONT Direct RNA Seq with the current Induro protocol, your #RNA may be partially degraded before it ever reaches the pore. A new preprint demonstrates that substantial RNA hydrolysis occurs with RT buffer conditions at 60°C and that uMRT at 30°C is a better solution. 🔗👇 #RNASky

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RNAConnect @rnaconnect.bsky.social · 21/05/2026

Detained introns. Alternative polyadenylation. Complex isoforms. Dr. Paul Boutz (U Rochester) showed at our recent webinar that LR-seq with uMRT captures what short-read methods miss — including in cancer transcriptomics. Watch now: bit.ly/4tNiZ1T #RNA #RNASky #ONT #nanoporeconf #RNASeq

Dr. Boutz (U Rochester / Wilmot Cancer Institute) used uMRT to reveal alternative splicing complexity and detained introns that short-read methods miss entirely.

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RNAConnect @rnaconnect.bsky.social · 19/05/2026

Your RNA-seq library prep may be hiding biology from you. We'll be at #RNA26 in Montréal showing how UltraMarathonRT reveals RNA biology that retroviral RTs miss. Come find us in the Exhibitor Hall or at our poster sessions. Details in the link below 👇 #RNASeq #RNA #RNASky

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RNAConnect @rnaconnect.bsky.social · 14/05/2026

Get more from the pore! RNAConnect Sr Sci and LR #RNASeq fanboy George Maio, PhD, gave a sneak preview of our newest #ONT #DRS and #cDNA data at the JAXLRSW earlier this week. Bottom line: More of those long isoforms you’ve been missing are now in reach. #RNA #RNASeq #Nanoporeconf #uMRT

UltraMarathonRT for ONT cDNA seq on UHRR

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RNAConnect @rnaconnect.bsky.social · 11/05/2026

#ONT #DRS is great for long #RNAs, unless they’re hydrolyzed during library prep. During this webinar, Dr. Underwood showed how uMRT outperforms other RTs during library prep. Full-length cDNA synthesis = more reads w/longer transcripts (shown here with human brain) bit.ly/4tNiZ1T #Nanoporeconf

Full-length cDNA synthesis means more reads spanning longer transcripts, shown here with human brain using uMRT.

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RNAConnect @rnaconnect.bsky.social · 11/05/2026

At #JAXLRSW26 this week? Catch us tomorrow, May 12: ▪ Talk @ 3:15 PM: Long transcript representation in #ONT #DRS with uMRT ▪ Posters @ 4:00–5:45 PM If you’re chasing isoforms, lncRNAs, or 3’ UTR structure, come talk to us directly or ✉️ info@rnaconnect.com. #RNASky #RNASeq @jax.org

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RNAConnect @rnaconnect.bsky.social · 29/04/2026

Heading to the JAX Long Read Sequencing Workshop, May 11–14? Connect with RNAConnect to discuss long-read RNA library prep, isoform detection, and getting more from your #PacBio or #ONT data. @jax.org #JAXLRSW26 #RNASeq

RNAConnect to sponsor Jackson Laboratory Long Read Sequencing Workshop 2026

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RNAConnect @rnaconnect.bsky.social · 13/04/2026

Attending the 2026 NCI RNA Workshop at the #NIH tomorrow? Don't miss our poster #21, "Improved Data Quality in Short Read and Long Read RNA-seq Using an Ultraprocessive Reverse Transcriptase That Copies at 30°C." We'd love to learn more about your research and see if we have tools that can help.

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RNAConnect @rnaconnect.bsky.social · 02/03/2026

Turn your toughest RNA-Seq challenges into publishable data with uMRT, which provides 10–30% more genes detected & truly uniform gene body coverage. Visit us at Booth #302 at #ABRF26, March 28–31 in Pittsburgh, PA! Learn more at the 🔗 in the comments. #RNASeq @abrf.bsky.social

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RNAConnect @rnaconnect.bsky.social · 25/02/2026

Missing isoforms? Uneven RNA-Seq coverage? Jason Underwood has answers today! @jg-underwood.bsky.social 📍 Poster 476 📅 Wed, Feb 25 | 4:45–6:10 p.m. New methods for more accurate, complete RNA-Seq data. Don't miss it at #AGBT26.

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RNAConnect @rnaconnect.bsky.social · 18/02/2026

Join us tomorrow (Feb 19) for a live discussion on overcoming key limitations in conventional #RNASeq, including incomplete transcript capture and structural bias, and exploring newer approaches that improve data resolution and confidence. Register via the link in the comments. #RNA #RNASky

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RNAConnect @rnaconnect.bsky.social · 12/02/2026

🎶 Where at #AGBT26 is…Jason Underwood? Find Jason. He’s the one introducing new methods for getting more accurate and complete #RNASeq data at Poster 476 on Feb 25, 4:45–6:10 p.m. Catch him → Schedule a partnership chat to explore integrations and joint studies. 👀🔗⬇️

RNAConnect at AGBT 2026. Find Jason Underwood VP of Technology Development.

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RNAConnect @rnaconnect.bsky.social · 29/01/2026

Methods and tools for RNA-seq are advancing rapidly. Are you aware of the drawbacks of historical methods and how you can improve resolution, reduce bias, and elevate confidence in your results? Learn more as our speakers share data and discuss the latest methods for #RNAseq. bit.ly/4rm6adZ

Webinar Graphic for Empowering New Transcriptomic Discoveries with Better Tools and Methods for RNA-Seq hosted on February 19, 2026, at 10 AM ET. Register Today.

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RNAConnect @rnaconnect.bsky.social · 07/01/2026

Now Available! The UltraMarathonRT® Direct RNA‑Seq Kit is compatible with your existing ONT protocol, designed to generate higher cDNA yields and improved performance on long/structured RNAs. Order now: bit.ly/4ju71Xl #RNA #RNAseq #NGS #nanopore #LongRead #RNASky

UltraMarathonRT Direct RNA-Seq Kit now available for ordering!

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RNAConnect @rnaconnect.bsky.social · 26/12/2025

Ditch the doomscrolling and read the latest data showing how uMRT can improve your ONT direct RNA-Seq. Highlights: • Notable gains for >5 kb • ↑ NIC & NNIC + ↑ intron retention • +~25% higher cDNA yield Dive into the data → www.biorxiv.org/content/10.6... #RNA #RNAseq #DRS #cDNA #RNASky

Read the latest RNAConnect publication titled Improved long-transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRT

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RNAConnect @rnaconnect.bsky.social · 16/12/2025

The UltraMarathonRT® Direct RNA‑Seq Kit preserves and detects longer #RNAs and reveals more isoforms per gene. This kit offers a simple drop‑in fit to your #Nanopore SQK‑RNA004 workflow. See how it expands what you can learn from every #longread seq sample. PR🔗below. #RNASeq #DRS

Press release: RNAConnect Launches the UltraMarathonRT Direct RNA-Seq Kit to Advance Nanopore-based Native-RNA Sequencing

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RNAConnect @rnaconnect.bsky.social · 13/11/2025

What if you could see more transcripts from each of your #RNA samples? uMRT has been engineered to efficiently copy even the most difficult RNA targets, including: 🔹 Highly structured RNAs 🔹 Long transcripts (≤20kb) 🔹 High GC content regions 🔹 Non-coding RNAs Learn more: bit.ly/488ScFO

More Data from Each RNA Sample with Less Bias UltraMarathonRT cDNA Synthesis & Amplification Kit

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RNAConnect @rnaconnect.bsky.social · 04/11/2025

Reveal What Retroviral RTs Miss. Unlock deeper RNA-seq insights with uMRT, including: 🔹 Low-abundance transcripts 🔹 High GC content regions 🔹 Unique isoforms 🔹 Non-coding RNAs ...and more Enhance your RNA-seq today! Read more in the comments below... #RNASeq #RNA #GeneDetection #BioTech

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RNAConnect @rnaconnect.bsky.social · 24/10/2025

uMRT has been engineered to deliver ultra-processivity at 30°C. It copies every RNA end-to-end in a single pass, which reveals more genes, transcripts, isoforms, and even twice as many retained introns as leading retroviral RT kits. Learn more: bit.ly/488ScFO #GeneDetection #RNASeq

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RNAConnect @rnaconnect.bsky.social · 22/10/2025

[1/2] A new publication from Warkentin & Pyle presents a practical workflow for accurate circRNA detection using UltraMarathonRT® (uMRT), a highly processive reverse transcriptase. Pub 🔗 in the comments. #cDNA #RNA #RNAseq #circRNA #RTPCR #RNASky

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RNAConnect @rnaconnect.bsky.social · 16/10/2025

We’re excited to (RNA)Connect with you at #ASHG25! Visit Booth #121 to discover how uMRT reveals more from every RNA sample. ▪️ 10–30% more genes detected ▪️ Superior quantification of long transcripts See what your #RNASeq data has been missing. #RNA #cDNA #RNASky #transcriptomics #genomics

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RNAConnect @rnaconnect.bsky.social · 15/10/2025

We’re live at ASHG 2025 — exhibit hours begin today from 9:30 am to 4:30 pm! Stop by Booth #121 to chat with the RNAConnect team and discover how UltraMarathonRT® can enable you to reveal more of the transcriptome. #ASHG25 #RNA #Genomics #Transcriptomics #RNASky

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RNAConnect @rnaconnect.bsky.social · 08/10/2025

Congrats to Chia-Ling Chou, Emmanuel Saliba, Anna Marie Pyle, Li-Tao Guo and co-authors on demonstrating how single-cell RNA-Seq with UltraMarathonRT® reveals unexpected transcriptomic diversity. 🔗👇 @emmanuel-saliba.bsky.social @litaoguo.bsky.social #singlecell #scRNAseq #RNA #RNAresearch #RNASky

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RNAConnect @rnaconnect.bsky.social · 01/10/2025

RNAseq has been limited by retroviral RTs for too long. The latest uMRT Kit reveals more information from every RNA sample. Unlock: ▪️ 10-30% more genes ▪️ More reliable gene quant ▪️ Improved gene body coverage with less bias ▪️ Detects 2x more retained introns #RNASeq #NGS #RNASky

The new UltraMarathonRT® (uMRT) cDNA Synthesis & Amplification Kit reveals more information from every RNA sample.

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RNAConnect @rnaconnect.bsky.social · 23/09/2025

You may be getting biased or inaccurate results from your RT-qPCR kit and not even know it. Our new white paper shows how UltraMarathonRT® delivers robust, reliable results regardless of primer choice and outperforms leading retroviral RTs. 🔗Link in comments. #RTqPCR #qPCR #RNASky

uMRT RT-qPCR provides more reliable results with consistent Ct values and full-length cDNA every time

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RNAConnect @rnaconnect.bsky.social · 09/09/2025

[1/2] Now Available! The new UltraMarathonRT® (uMRT) cDNA Synthesis & Amplification Kit delivers better gene detection and quantification. #RNAseq #RNASky #Transcriptomics #ReverseTranscriptase #NGS

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RNAConnect @rnaconnect.bsky.social · 04/09/2025

Join us at booth 121 during #ASGH25 to learn how uMRT can provide you with more accurate gene expression profiles and reveal more isoforms, novel splicing, and previously undetectable intron retention events. #ASHG2025 #RNA #RNASky #Transcriptomics #IntronRetention #Genomics #RNASeq #NGS

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RNAConnect @rnaconnect.bsky.social · 27/08/2025

Now available: UltraMarathonRT® cDNA Synthesis & Amplification Kit! Elevate short-read #RNAseq with unmatched processivity: detect rare isoforms & #lncRNAs, quantify long transcripts, and integrate seamlessly with Illumina®-style workflows. Press release🔗in the comments. #RNASky #transcriptomics

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RNAConnect @rnaconnect.bsky.social · 20/08/2025

[1/2] "The 21st century, Thomas Cech says, 'is the age of noncoding RNA.'" We agree with Dr. Cech. Since ncRNAs can be long, structured, and modified, they’re often tricky to characterize. So, we developed the solution: a next-generation RT, UltraMarathonRT. #RNASky #RNA #ncRNA #noncodingRNA

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RNAConnect @rnaconnect.bsky.social · 01/08/2025

We’re celebrating #RNADay by spotlighting the four winners of the RNAConnect 5K Sprint Award! 🥇 First Place Dr. Melissa Jurica (UC Santa Cruz) Dr. Daniel Kiss (Houston Methodist) 🥈 Second Place Dr. Andrew Berglund (Univ at Albany) Dr. Mark Williams (Yale School of Medicine) #RNASky

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RNAConnect @rnaconnect.bsky.social · 14/07/2025

Applications for the RNAConnect 5K Sprint Award close tonight, July 14th! Don't miss out on this chance to propel your RNA research forward. Link in the comments! Apply now! #RNA #RNAConnect #5KSprintAward #RNAResearch #ScienceAward #ScienceFunding #ResearchGrants #RNASky

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RNAConnect @rnaconnect.bsky.social · 12/06/2025

Applications are NOW OPEN for the RNAConnect 5K Sprint Award! Compete for a $5,000 cash prize to support your innovative RNA research. 🔗Learn more: bit.ly/3H80DWD #RNA #RNAResearch #ScienceAward #ScienceFunding #ResearchGrants #RNASky

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RNAConnect @rnaconnect.bsky.social · 30/05/2025

We're looking forward to another productive day at #RNA25! Join us Friday morning at 7:45am for Breakfast & Breakthroughs plus engaging poster sessions in the evening featuring the latest innovations with UltraMarathonRT. #RNA2025 #RNAresearch #Transcriptomics #RNASky

RNAConnect presents at RNA Society Meeting 2025

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