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RAPP OptoElectronic @rappopto.bsky.social · 09/10/2026

2/2 🎯 IR-only for gentle capture ⚡ UV + IR for fast, precise cut-and-capture ✂️ UV-only for cutting workflows without capture ➡️ The point is simple: the LCM mode should fit the experiment. Not the other way around.

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RAPP OptoElectronic @rappopto.bsky.social · 09/10/2026

1/2 😧 IR capture, UV cut, or both? In #LaserCaptureMicrodissection, the best workflow depends on the sample, the target region, and the downstream application. With Accuva Cellect, different #LCM modes can be matched to different experimental needs ⤵️

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RAPP OptoElectronic @rappopto.bsky.social · 05/10/2026

🔬This week at @cssbhamburg.bsky.social, we’re testing #holotomography on real participant samples: from bacteria and biofilms to cells, 3D cultures, tissues, and whole organisms. ✨Our carousel gives a quick technical overview of the method and the @tomocube.bsky.social HT-X1™ Plus. #LabelFreeImaging

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RAPP OptoElectronic @rappopto.bsky.social · 01/10/2026

🔬 High-res 3D imaging without labels: #holotomography uses refractive index as intrinsic contrast for quantitative imaging of living samples. Next week at @cssbhamburg.bsky.social , we’ll demo the @tomocube.bsky.social HT-X1™ Plus hands-on with participant samples. Book your spot: shorturl.at/Pu2U3

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RAPP OptoElectronic @rappopto.bsky.social · 29/09/2026

🧑‍🔬Am 3.10. sieht es bei RAPP ein bisschen anders aus als sonst. Gemeinsam folgen wir dem Licht: von den Grundlagen der Optik bis in den biologischen Mikrokosmos. 🔗Mikroskopie selbst erleben: ein Gedanke, den wir auch mit @gerbi-gmb.de teilen. #TürenAufMitDerMaus #Wissenschaftskommunikation #WissKomm

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RAPP OptoElectronic @rappopto.bsky.social · 25/09/2026

3/3 🧩 At RAPP, we configure BOB™ around your experimental requirements and can expand it with our modules for #photomanipulation, #optogenetics, #FRAP, and holographic #photostimulation with Holo4D. ➡️ BOB™ adapts to your experiment. Not the other way around.

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RAPP OptoElectronic @rappopto.bsky.social · 25/09/2026

2/3 🔀 BOB™ can support in vivo and in vitro workflows, adjustable working height, motorized focus, flexible stage/translator options, and contrast methods such as epifluorescence, DIC, and OCC. ✅ Built for flexible #bioimaging setups.

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RAPP OptoElectronic @rappopto.bsky.social · 25/09/2026

1/3 💡 What makes Sutter Instrument’s BOB™ stand out as a #Microscopy platform? It removes the conventional microscope frame and creates an open-design upright platform that can adapt to the experiment.

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RAPP OptoElectronic @rappopto.bsky.social · 22/09/2026

3/3 📷 Can you verify what was selected, removed, and captured? ⚙️ Does it support the steps after isolation? Selecting an LCM system is about fit: with your specimen, capture requirements, and downstream workflow.

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RAPP OptoElectronic @rappopto.bsky.social · 22/09/2026

2/3 🧫 Can it handle your actual tissue, cell, or preparation format? 🎯 Do you need UV cutting, or is IR capture-only more appropriate? 🧪 Which preparation strategy is easiest, reliable, and cost-effective for your sample?

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RAPP OptoElectronic @rappopto.bsky.social · 22/09/2026

1/3 🤔 How do you know whether an #LCM system actually fits your workflow? A useful place to start: specimen type, capture strategy, sample preparation, sample custody, and downstream analysis. #LaserCaptureMicrodissection

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RAPP OptoElectronic @rappopto.bsky.social · 17/09/2026

2/2 Instead of raster-scanning a larger ROI line by line, the selected shape can be illuminated simultaneously and freely positioned within the field of view. ➡️ In short: defined shapes, flexible positioning, and simultaneous illumination for larger ROIs.

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RAPP OptoElectronic @rappopto.bsky.social · 17/09/2026

1/2 ✨ In some #photomanipulation workflows, it is beneficial to illuminate larger ROIs. 👉 That is the unique feature of the UGA-42 Geo: The system reshapes the laser spot into 40 preset hardware-defined illumination geometries, including 🟢 circles, 🟨 squares, and ⭕ donuts in different sizes.

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RAPP OptoElectronic @rappopto.bsky.social · 15/09/2026

🐭 Kinder haben diese wunderbare, unerschöpfliche Neugier. Dafür öffnen wir unsere Türen & geben einen spielerischen Einblick in Licht, Optik & den biologischen Mikrokosmos. 🔬 Die Begeisterung für Mikroskopie & Wissenstransfer teilen wir auch mit @gerbi-gmb.de #Wissenschaftskommunikation #WissKomm

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RAPP OptoElectronic @rappopto.bsky.social · 10/09/2026

Häring et al., Biophysical Journal (2026): doi.org/10.1016/j.bp... Armstrong et al., EMBO Reports (2026): doi.org/10.1038/s443... Boonen et al., Nature Communications (2026) : doi.org/10.1038/s414...

doi.org

Redirecting

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RAPP OptoElectronic @rappopto.bsky.social · 10/09/2026

Wang et al., Neuron (2026) : doi.org/10.1016/j.ne... Rais et al., Progress in Neurobiology (2026): doi.org/10.1016/j.pn... Mosbach et al., Chemistry – A European Journal (2026): doi.org/10.1002/chem...

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RAPP OptoElectronic @rappopto.bsky.social · 10/09/2026

🔬 Different papers, different biological questions, yet one shared core principle: #microscopy can go beyond observation alone. Exciting to see advanced #photomanipulation tools used so creatively in research. 👏 Congrats to all authors. ⬇️ Links to papers below.

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RAPP OptoElectronic @rappopto.bsky.social · 07/09/2026

3/3 🔬 With #Holo4D, one- or multi-photon imaging can run in parallel. ↔️ Flexible in/out triggering and auxiliary-device compatibility help connect it to complex setups. ✅ Holo4D: Precise, simultaneous pattern photostimulation into experiments where space, timing, and biological complexity matter.

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RAPP OptoElectronic @rappopto.bsky.social · 07/09/2026

2/3 🧠 This is especially useful when the spatial organization of the sample matters in 2D, 3D, and across time. Researchers can target single cells, multiple cells, or defined clusters to study neural circuits, cellular interactions and dynamic biological processes.

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RAPP OptoElectronic @rappopto.bsky.social · 07/09/2026

1/3 ✨ What is holographic #photostimulation? It uses spatial light modulation (SLM) to shape a laser beam into defined light patterns, enabling tunable 3D illumination and simultaneous targeting of multiple selected ROIs within the field of view.

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RAPP OptoElectronic @rappopto.bsky.social · 04/09/2026

✨ Manchmal sind es die kleinsten Dinge, die die größten Fragen auslösen. Am 3.10. laden wir Kinder ein, Optik und Mikroskopie selbst zu entdecken. 🔗 Ein Thema, das uns auch mit @gerbi-gmb.de verbindet: Mikroskopie verständlich und zugänglich machen. #WissKomm #Wissenschaftskommunikation

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RAPP OptoElectronic @rappopto.bsky.social · 04/09/2026

Very happy to see our colleague Simona Stelea presenting at @ceitec.eu’s @ceitec-cellimcf.bsky.social at @masarykuniversity.bsky.social in Brno on the September, 22! 😊 ✨ A great chance to explore how #photomanipulation turns light into an active tool in microscopy. Thanks for hosting! 🙏

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RAPP OptoElectronic @rappopto.bsky.social · 02/09/2026

4/4 📷 Verify and document. The integrated QC station enables visual verification and step-by-step documentation of captured material. ✅ Because in #LCM, confidence comes not only from what you select, but from knowing what was actually captured.

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RAPP OptoElectronic @rappopto.bsky.social · 02/09/2026

3/4 🧫 Prepare flexibly: FFPE, fresh-frozen, fluorescent, stained or unstained samples, thin or thick sections. 🧩 Choose the slide format that fits your workflow: plain glass, glass/frame membrane slides, or Petri dishes for live-cell work.

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RAPP OptoElectronic @rappopto.bsky.social · 02/09/2026

2/4 👉 That is the idea behind #AccuvaCellect: Cut. Capture. Verify. ⚡ Cut: fast, precise UV cutting. 🎯 Capture: gentle IR capture for selected ROIs, with or without prior cutting.

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RAPP OptoElectronic @rappopto.bsky.social · 02/09/2026

1/4 #LaserCaptureMicrodissection enables the isolation of selected cells or tissue regions for downstream analysis. But selection is only part of the workflow. The key question: can you verify that the selected material was actually captured?

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RAPP OptoElectronic @rappopto.bsky.social · 27/08/2026

Deep-UV enables: 🔬imaging and fluorescence excitation below 330 nm 🧬localized DNA damage and repair studies ⚡photoactivation and UV-based #photomanipulation workflows 📄Find multiple publications showcasing Deep-UV microscopy and photomanipulation in real research applications on the RAPP website.

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RAPP OptoElectronic @rappopto.bsky.social · 27/08/2026

2/3 ⚙️For Deep-UV #microscopy, the relevant light path needs to be modified with suitable UV-transmitting optics and paired with appropriate objectives. 👉At RAPP, we are uniquely positioned to modify new microscopes and retrofit existing systems to make them suitable for transmission down to 190 nm.

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RAPP OptoElectronic @rappopto.bsky.social · 27/08/2026

1/3 🔬 X 🟣 = ⁉️ Can your #microscope handle Deep-UV? Standard microscope optics and objectives are usually not designed to transmit light below approx. 330 nm. That means that even if a Deep-UV source is available, the microscope itself may limit what actually reaches the sample.

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RAPP OptoElectronic @rappopto.bsky.social · 25/08/2026

5/5 📄 We put together a short carousel highlighting some of the key findings and the optical setup behind the experiments. The full paper contains more detail than we could include here, so we highly recommend reading it in full: doi.org/10.1002/chem...

doi.org

Infrared Laser Heating Reveals a Reversible Phase Transition of Phosphofructokinase 1 in Mammalian Cells

In this study, we present a novel method to investigate the phase behavior of biomolecular condensates in living cells. The method complements FRAP experiments, expanding the existing toolbox for cha....

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RAPP OptoElectronic @rappopto.bsky.social · 25/08/2026

4/5 🎯 Great to see a RAPP-customized laser T-jump setup used in this work. Local IR heating adds a functional phase-transition readout to live-cell condensate studies and complements #FRAP. 👏 Congratulations to Morten Mosbach, Sneha Pavanan Kuruvanthara, Lukas Johannknecht, and Simon Ebbinghaus.

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RAPP OptoElectronic @rappopto.bsky.social · 25/08/2026

3/5 🔬 X 🔴 = 🌡️ The authors combine confocal imaging with localized IR laser heating to perturb PFKL glucosomes in living HeLa cells with subcellular precision. Glucosomes dissolve upon heating and re-form after cooling, even when glucosome mobility is strongly reduced under osmotic stress.

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RAPP OptoElectronic @rappopto.bsky.social · 25/08/2026

2/5 💡PFKL catalyzes the rate-limiting step of #glycolysis. Glucosomes are membraneless multienzyme assemblies that may help direct glucose flux between energy production and biosynthetic pathways; especially relevant in highly proliferative cells where glucose metabolism is often rewired.

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RAPP OptoElectronic @rappopto.bsky.social · 25/08/2026

1/5 🤔 How can we study biomolecular condensate phase behavior directly inside living cells? In a Chemistry – A European Journal paper by @chemistryeurope.bsky.social, Mosbach et al. investigate PFKL glucosomes: dynamic condensates linked to glucose metabolism.

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RAPP OptoElectronic @rappopto.bsky.social · 19/08/2026

3/3 🧩 At RAPP, we develop modular optical devices that connect imaging with controlled light-based intervention. The #microscope becomes more than a window into the sample. It becomes an experimental interface. 👉 In one word: #Photomanipulation

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RAPP OptoElectronic @rappopto.bsky.social · 19/08/2026

2/3 Today, light can do more than reveal structures and processes. 👉 Use light to perturb, stimulate, modify or locally control what happens inside a sample with spatial and temporal precision.

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RAPP OptoElectronic @rappopto.bsky.social · 19/08/2026

1/3 🔬 In #microscopy, the mantra has long been: seeing is believing. Much of modern #bioimaging has pushed resolution, sensitivity and speed. ⚡ But microscopy can go beyond observation.

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RAPP OptoElectronic @rappopto.bsky.social · 12/08/2026

3/3 🔬 Flexible slide options, including plain glass slides, glass and frame membrane slides, as well as Petri dishes for live-cell work 📷 Integrated visual QC, supporting step-by-step documentation of captured material

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RAPP OptoElectronic @rappopto.bsky.social · 12/08/2026

2/3 The system combines: 🔴 X 🟣 UV cutting and IR capture for different sample and ROI requirements 🧫 Compatibility with diverse sample preparations, including FFPE, fresh-frozen, fluorescent, stained, or unstained material

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RAPP OptoElectronic @rappopto.bsky.social · 12/08/2026

1/3 👉 The Accuva Cellect by @Laxco, Inc. is purpose-built for #LaserCaptureMicrodissection workflows where precision, preparation flexibility, and sample verification matter. 📖 Learn more about the Accuva Cellect #LCM system and possible workflows on the RAPP website.

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RAPP OptoElectronic @rappopto.bsky.social · 04/08/2026

3/3 The series includes: 🎯 AiWon: high-power point spot 🔷 AiWon Geo: shaped ROIs without scanning ⚡ AiWon Caliburn: pulsed-laser ablation, microdissection & DNA damage ➡️ Different applications. Different light sources. One modular platform.

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RAPP OptoElectronic @rappopto.bsky.social · 04/08/2026

2/3 💡 That’s the idea behind AiWon: modular fixed-spot photomanipulation. 🔬 AiWon integrates into the fluorescence beam path without modifying the microscope. Illumination stays fixed in the FOV center, while the sample is positioned by moving the stage.

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RAPP OptoElectronic @rappopto.bsky.social · 04/08/2026

1/3 👉 Not every #photomanipulation experiment needs rapid beam positioning or simultaneous illumination of multiple areas. For many workflows, local light delivery in the center of the FOV is exactly what is needed.

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RAPP OptoElectronic @rappopto.bsky.social · 30/07/2026

3/3 🧩 In RAPP fashion, Holo4D is a flexible add-on for (advanced) microscope platforms incl. Sutter MOM, MIMMS & BOB. ⚙️ Automated 2D/3D calibration, SysCon, ScanImage handshaking and device integration help connect #photomanipulation to complex experiments.

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RAPP OptoElectronic @rappopto.bsky.social · 30/07/2026

2/3 ⚡ Especially relevant for investigating neural circuits, cellular interactions, and dynamic biological processes where the position, timing, and pattern of stimulation matter. 🌈 Holo4D’s SLM functions across a wide range of wavelengths providing ample experimental flexibility.

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RAPP OptoElectronic @rappopto.bsky.social · 30/07/2026

1/3 💡 What if #photostimulation could match the 3D structure of your sample? Holo4D uses SLM-based holographic beam shaping to project defined light patterns onto selected cells or clusters, bridging #multiphoton imaging with targeted 3D stimulation.

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RAPP OptoElectronic @rappopto.bsky.social · 28/07/2026

2/2 🔥 This is where biology meets physics. 🎯 Using IR light near a strong water-absorption band, heat can be induced locally and on demand: where and when it is needed during #LiveCellImaging. ✅Useful for #thermogenetics, heat-shock responses, cytoskeleton dynamics, motor proteins & #biophysics.

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RAPP OptoElectronic @rappopto.bsky.social · 28/07/2026

1/2 🌡️ What if temperature control became your next #photomanipulation tool? Temperature plays a central role in biology. It can influence protein activity, molecular interactions, reaction rates, and cellular behavior. 🔍 Many questions need more precision than whole-sample heating can provide.

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RAPP OptoElectronic @rappopto.bsky.social · 23/07/2026

3/3 ⚡ At RAPP, we help combine 2P imaging with #photostimulation, #optogenetics and #photomanipulation. 🧠 Use it for in vivo/in vitro 2P whole-cell/intracellular imaging, or calcium activity. Typical samples are cultured cells, brain slices, organoids, and even zebrafish.

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RAPP OptoElectronic @rappopto.bsky.social · 23/07/2026

2/3 🧩 DF-Scope® keeps the BX51WI platform in place and allows photostimulation light sources to be added directly into the main scanned laser path. 💡 That means experiments can move beyond #bioimaging toward active optical interaction with the sample.

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