iiinova — where your digital self keeps evolving

Reposted by Ivan Radin

Molecular Plant & Plant Communications @mplantpcom.bsky.social · 08/10/2026

Sensing water excess: COSR channels mediate hypoosmotic Ca2+ signaling in plants #spotlight #MolecularPlant cell.com/molecular-pl...

055

Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 13/07/2026

The 5th Annual PCA Core Network Participant (CNP) Meeting took place this past weekend in St. Louis, MO! It was a great opportunity to celebrate accomplishments from the past year, connect with colleagues from across the network, and discuss the next phase of the PCA.

193

Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 10/06/2026

PCA Members are excited to share our new paper, which outlines a roadmap for tracing the origins & diversification of cell types across green plants using single-cell technologies. From algae to angiosperms, we're entering a new era of comparative cell biology. bit.ly/4eAip2Q

bit.ly

A single-cell blueprint for cellular diversity in the Green Lineage

The Green Lineage (Viridiplantae) represents one of the most successful evolutionary radiations on Earth, with remarkable morphological diversity from…

186

Ivan Radin @radinbio.bsky.social · 11/05/2026

The fall (black to red to orange to yellow to white) look-up table has been applied. There is a significant variability in expression levels between cells, so some are much dimmer. Due to the leaf's uneven surface, some cells appear to be in different layers.

020

Ivan Radin @radinbio.bsky.social · 11/05/2026

The image was captured on an Olympus/Evident Confocal FV3000 with HyD detectors using a 60x/1.2W objective. A small section of the live leaf was mounted in water. The final image is a cropped region of a larger 3-by-3-stitched Z-stack image that was deconvolved.

120

Ivan Radin @radinbio.bsky.social · 11/05/2026

These epidermal cells of tobacco (Nicotiana benthamiana) leaf are expressing cytosolic GFP. The brightest region is the nucleus, which is surrounded by a large central vacuole. The cytosol is restricted to the cell periphery. Transvacuolar strands connect the nucleus with other parts of the cell.

Fluorescence microscopy image showing glowing red cells with bright yellow centers and branching extensions against a black background; scale bar indicates 20 µm.

1133

Ivan Radin @radinbio.bsky.social · 06/05/2026

In case you missed it, the second Plant Imaging Workshop organized by @plantcellatlas.bsky.social is now available on YouTube: www.youtube.com/watch?v=rytH... In this session, you will learn how to get started with MorphoGraphX to do 2D and 3D segmentation. #plantmicroscopy #plantimaging

youtube.com

Imaging Workshop Webinar Series Part 2: Introduction to 2D and 3D Segmentation with MorphoGraphX

YouTube video by Plant Cell Atlas

0157

Ivan Radin @radinbio.bsky.social · 27/04/2026

The image was captured on a Leica THUNDER Imager Model Organism dissecting scope (M205FCA).

010

Ivan Radin @radinbio.bsky.social · 27/04/2026

This is a section of juvenile moss (Physcomitrium patens) tissue, called protonemata. The apical cells expand by tip-growth, while subapical cells sometimes divide to make lateral filaments. Cytosolic GFP is in green, and chlorophyll autofluorescence is in magenta. #microscopymonday #moss

Fluorescence microscopy image showing a dense network of thin, branching filamentous structures, colored in green and magenta against a black background. The filaments overlap and extend in multiple directions. A scale bar in the lower right corner indicates 250 µm.

25210

Ivan Radin @radinbio.bsky.social · 06/04/2026

Thank you. And yes we very much support the use of accessible colors 😄

010

Ivan Radin @radinbio.bsky.social · 06/04/2026

Image was captured on the FV300 confocal microscope with a 60x/W NA 1.2 objective from Evident/Olympus. This is a Z-max intensity projection of a deconvolved stack.

130

Ivan Radin @radinbio.bsky.social · 06/04/2026

An undergraduate student in the lab, Andy Marchant, made this awesome image of a moss (Physcomitrium patens) apical chloronema cell. This is a beautiful example of how the ER network looks in plant cells (in green). The chlorophyll autofluorescence is in magenta. #microscopymonday #moss

Fluorescence microscopy image showing a green cellular ER network with magenta oval chloroplasts on a black background, with a 3 µm scale bar.

1195

Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 31/03/2026

We are a month away from the second installment of the Plant Cell Atlas Imaging Workshop Series: Introduction to 2D and 3D Segmentation with MorphoGraphX! Don't forget to register! plantcellatlas.org/events

Promotional image for the PCA Imaging Workshop Webinar Series: Introduction to 2D and 3D Segmentation with MorphoGraphX. The promo has a purple background behind text over a green plant cell graphic with a black background. It also includes the details for the webinar including the date/time.

141

Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 25/03/2026

The Plant Science Art Exhibit is now on display at the University of Minnesota's College of Biological Sciences! This exhibit will be up for the rest of the semester to engage students and inform them about the history and potential of plant science! Learn more about the exhibit: bit.ly/4byqtQo

An art poster of George Washington Carver displayed in a university hallway.

An art poster on Phytoremediation displayed in a university hallway.

262

Reposted by Ivan Radin

Tamara Hernández-Verdeja @tamara-hverdeja.bsky.social · 23/03/2026

🌱💚🎉 Sharing (again 😁) my review on regulation of chloroplast biogenesis. First work of my emerging research group at @cbgpmadrid.bsky.social

0178

Ivan Radin @radinbio.bsky.social · 23/03/2026

Guard cells form the stomata pore, a microscopic opening on the surface of leaves and stems. This is the main site of gas exchange (O2 and CO2) and transpiration (water release). The remaining epidermal cells surrounding the guard cells are not visible here.

000

Ivan Radin @radinbio.bsky.social · 23/03/2026

I pulled this image from the archives of my PhD work, back when I was working with plant and yeast mitochondria. These are two Arabidopsis guard cells, with their cytosol in magenta, mitochondria in yellow, and chlorophyll autofluorescence in green. #microscopymonday, #plantcells, #guardcells

A fluorescence image showing two curved, kidney-shaped plant guard cells forming a stomatal pore. The cells glow in bright magenta, outlining their overall shape, while clusters of distinct round and irregular structures inside them fluoresce green and yellow. The background is completely black.

1122

Ivan Radin @radinbio.bsky.social · 09/03/2026

This is a maximum-intensity projection along Z from a deconvolved stack captured on a confocal FV3000 (Evident/Olympus) microscope with a 60x water (NA 1.2) objective.

040

Ivan Radin @radinbio.bsky.social · 09/03/2026

Moss (Physcomitrium patens) protoplasts (wallless cells) transformed with chloroplast envelope protein tagged with mGFP (green). The chlorophyll autofluorescence is in magenta. The protrusions of the chloroplast envelope are called stromules. #microscopymonday, #plantcells, #plantmicroscopy, #moss

Fluorescence microscopy image of a spherical cluster of chloroplasts, showing magenta-stained interiors surrounded by bright green outlines against a black background.

16011

Ivan Radin @radinbio.bsky.social · 09/03/2026

This is a maximum-intensity projection along Z from a deconvolved stack captured on a confocal FV3000 (Evident/Olympus) microscope with a 60x water (NA 1.2) objective.

000

Ivan Radin @radinbio.bsky.social · 23/02/2026

While the epidermis is a continuous layer, the leaf's uneven surface and differences in cell expression levels give it the appearance of multiple layers. This stitched image (multiple fields of view merged together) was captured on an FV3000 confocal microscope from Evident/Olympus.

000

Ivan Radin @radinbio.bsky.social · 23/02/2026

Surface (epidermal) cells of many plants are shaped like jigsaw puzzle pieces. 🧩 These are epidermal cells of tobacco (Nicotiana benthamiana) expressing cytosolic EGFP. The bright spherical structures are cell nuclei. #microscopymonday, #plantcells, #plantmicroscopy

Fluorescent microscope image showing a dense network of bright green, irregularly shaped plant cells with glowing outlines against a dark background.

1112

Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 19/02/2026

Registration is NOW OPEN for the second installment of the Plant Cell Atlas Imaging Workshop Series: Introduction to 2D and 3D Segmentation with MorphoGraphX. You can learn more and register here: www.plantcellatlas.org/events

187

Ivan Radin @radinbio.bsky.social · 10/02/2026

That's a great question. Unfortunately, we didn't continue imaging these samples, so I don't know.

110

Ivan Radin @radinbio.bsky.social · 10/02/2026

I am not sure, but it would be cool. I will need to look into that.

110

Ivan Radin @radinbio.bsky.social · 09/02/2026

We were testing lasers and the spectral ability of our confocal (FV3000) on a zoomed-in section of a Brassica rapa leaf. But when we zoomed out, we saw that the lasers we used photobleached chlorophyll autofluorescence in the square area we had zoomed in on. We used a 10x/0.4 objective for this.

Red fluorescence microscopy image showing a dense, mottled red signal across the field with a distinct dark, square-shaped region at the center where fluorescence is absent or reduced; a 100 µm scale bar appears in the lower-right corner.

1143

Ivan Radin @radinbio.bsky.social · 26/01/2026

These Brewer's yeast cells were in a hurry! This was captured with a point-scanning confocal microscope, which scans one point (pixel) at a time, moving from left to right and top to bottom. As a consequence, cells moving faster than the scan speed appear as streaks or exhibit distortions.

Image shows a gray-scale image of many circular-shaped yeast cells. There are also many streaks caused by fast-moving cells.

021

Reposted by Ivan Radin

FocalPlane @focalplane.bsky.social · 16/01/2026

Our featured image shows epidermal cells of a tobacco leaf expressing cytosolic GFP. The image was acquired by Ivan Radin @radinbio.bsky.social. You can read more about the image and Ivan’s research in our post ⤵️ focalplane.biologists.com/2026/01/16/f... #FluorescenceFriday

focalplane.biologists.com

Featured image with Ivan Radin - FocalPlane

Featured image with Ivan Radin - News

073

Ivan Radin @radinbio.bsky.social · 06/01/2026

I absolutely love this paper from @stuartmcdaniel.bsky.social It is a part of a must-read list for all new members of my lab. Bryophytes are not early diverging land plants - McDaniel - 2021 - New Phytologist - Wiley Online Library nph.onlinelibrary.wiley.com/doi/10.1111/...

nph.onlinelibrary.wiley.com

Bryophytes are not early diverging land plants

Phylogenetic trees have permeated biology. However, an understanding of how to interpret phylogenies has lagged behind, notably in publications outside of evolutionary biology. Here I argue that some...

0162

Ivan Radin @radinbio.bsky.social · 05/01/2026

This is a deconvolved Z-maximum projection of an image captured on the FV3000 confocal microscope from Olympus using a 60x/1.2W objective.

030

Ivan Radin @radinbio.bsky.social · 05/01/2026

Happy New Year, everyone. For this #microscopymonday, here is a moss (Physcomitrium patens) protoplast (wall-less cell) transformed with a nuclear marker (in magenta). The much brighter area is the nucleolus. The chlorophyll autofluorescence is in green. #moss #plantcells

The image shows a round protoplast filled with green, evenly distributed ovoid-shaped chloroplasts. In the center of the cell is the spherical nucleus in magenta.

1233

Reposted by Ivan Radin

Harmit Singh Malik @harmitmalik.bsky.social · 01/01/2026

Today I made a small but momentous start to work in 2026 by changing a single number. I renamed the file “Papers to write and submit in 2025” to “Papers to write and submit in 2026”. Stay tuned for more file updates on 1st January 2027.

1032425

Reposted by Ivan Radin

Ben Engel @cellarchlab.com · 28/12/2025

EMPIAR-11830 #ChlamyDataset #TeamTomo 🧪 www.cell.com/molecular-ce...

0212

Reposted by Ivan Radin

Journal of Experimental Botany @jxbotany.bsky.social · 27/12/2025

🔬 EXPERT VIEW 🔬 In this review, Cox & Czymmek cover the recent developments of expansion microscopy techniques in plant systems and provides examples of their applications in plant biology research. 🔗 doi.org/10.1093/jxb/... #PlantScience 🧪 @kcox-bioguy.bsky.social

Fig. 1.Current expansion microscopy (ExM) methods in plant systems and other potential applications. Figure shows a schematic diagram of digested, permeabilized, expanded plant organelles, cells, or tissues embedded in a hydrogel in the center. Left panel showcases ExM techniques successfully applied in plant systems. These include chloroplast and nuclei (isolated organelles), Chlamydomonas, tobacco BY-2 cells, protoplasts, Arabidopsis seed embryos, and Arabidopsis roots. Right panel outlines other potential applications of ExM in other areas of plant biology including developmental biology, plant-microbe interactions, and spatial and single-cell biology. Created in BioRender. Cox, K. (2025) https://BioRender.com/ok72cvy.

13716

Reposted by Ivan Radin

New Phytologist @newphyt.bsky.social · 28/12/2025

Our #VirtualIssue on Plant terrestrialization focuses on piecing together #streptophyte trait #evolution and curating research that has contributed to advances in the evolutionary inference of (early) land plant form and function 👇 📚 nph.onlinelibrary.wiley.com/doi/toc/10.1... #PlantScience 🧵1/2

Streptophyte terrestrialization and the conquest of land by embryophytes. Illustration adapted from Fig. 1 of de Vries & Archibald (2018; https://doi.org/10.1111/nph.14975) by Debbie Maizels.

1144

Reposted by Ivan Radin

Journal of Cell Biology @jcb.org · 24/12/2025

A crucial step towards understanding tip growth in plants. Ivan Radin @radinbio.bsky.social (University of Minnesota) highlights work from Ryken et al. of the Bezanilla lab (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/... #CellSignaling #PlantBiology #PlantCellBiology

0123

Ivan Radin @radinbio.bsky.social · 22/12/2025

These amazing plants grow as small rosettes, and their leaves are covered with tentacles that secrete mucilage on the tips. The plants use the mucilage to adhere the insect prey long enough for the tentacles and the leaf to curve and entrap the prey.

The image shows one Sundew plant in a pot surrounded by small green moss plants. The sundew plant is reddish in color and has leaves covered with sticky tentacles.

010

Ivan Radin @radinbio.bsky.social · 22/12/2025

These close-ups of mucilage-covered tentacle tips from the leaf of a carnivorous sundew plant (Drosera spatulata) definitely have a strong holiday vibe, so I am posting them just in time for the holidays. Happy Holidays. #microscopymonday #carnivorousplants

The image shows a close-up of the tentacle tip from the leaf of a carnivorous sundew plant (Drosera spatulata). Tentacles are reddish in color and have a thin stalk with a large head at the end, which secretes a drop of clear mucilage that completely surrounds it.

The image shows a close-up of the tentacles from the leaf of a carnivorous sundew plant (Drosera spatulata). One is in the foreground and in focus, while the rest are blurry in the background. Tentacles are reddish in color and have a thin stalk with a large head at the end, which secretes a drop of clear mucilage that completely surrounds it.

161

Reposted by Ivan Radin

Journal of Cell Biology @jcb.org · 17/12/2025

Spotlight: Ivan Radin discusses new work from Ryken and colleagues (rupress.org/jcb/article/...) which shows how localization and function of autoinhibitory calcium ATPases maintains the strength of the tip-focus Ca2²⁺ gradient during polarized plant growth. rupress.org/jcb/article/... #PlantBiology

0115

Ivan Radin @radinbio.bsky.social · 15/12/2025

Very true, but not all plant chloroplasts respond this way to beta-lactams, as likely not all plants have the peptidoglycan layer.

010

Ivan Radin @radinbio.bsky.social · 15/12/2025

This is a deconvolved Z-maximum projection of an image captured on the FV3000 confocal microscope from Olympus using a 60x/1.2W objective. 3/3

020

Ivan Radin @radinbio.bsky.social · 15/12/2025

Magenta - chlorophyll autofluorescence, Green -mGFP-tagged protein targeted to the chloroplast envelope. The envelope can produce tubular protrusions called stromules, many of which are visible here. These are moss gametophore cells, many of which have only one giant chloroplast. 2/3

130

Ivan Radin @radinbio.bsky.social · 15/12/2025

This is what happens to the moss (Physcomitrium patens) chloroplasts when we grow cells on β-lactam antibiotics. β-lactams inhibit the synthesis of the peptidoglycan in the chloroplast envelope, leading to their dramatic expansion. #microscopymonday #moss #plantcells #plantmicroscopy 1/3

The image shows large moss chloroplasts organized in irregular rows. The chloroplasts, in magenta, are somewhat rectangular in shape but with many irregularities. Many green tubular protrusions extend from the chloroplast surface.

1192

Ivan Radin @radinbio.bsky.social · 01/12/2025

This is a maximum projection of a Z-stack captured on the FV3000 point scanning confocal system using a 60x/1.3 silicon oil objective and subjected to deconvolution.

000

Ivan Radin @radinbio.bsky.social · 01/12/2025

The plastids in the epidermal cells are smaller and accumulate more RecARed marker, hence are very bright. These plastids also produce a lot of protrusions, called stromules. The plastids from the deeper cells are bigger and have much more chlorophyll, so they appear greyer.

100