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Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 13/07/2026

The 5th Annual PCA Core Network Participant (CNP) Meeting took place this past weekend in St. Louis, MO! It was a great opportunity to celebrate accomplishments from the past year, connect with colleagues from across the network, and discuss the next phase of the PCA.

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Plant Cell Atlas @plantcellatlas.bsky.social · 10/06/2026

PCA Members are excited to share our new paper, which outlines a roadmap for tracing the origins & diversification of cell types across green plants using single-cell technologies. From algae to angiosperms, we're entering a new era of comparative cell biology. bit.ly/4eAip2Q

bit.ly

A single-cell blueprint for cellular diversity in the Green Lineage

The Green Lineage (Viridiplantae) represents one of the most successful evolutionary radiations on Earth, with remarkable morphological diversity from…

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Ivan Radin @radinbio.bsky.social · 11/05/2026

These epidermal cells of tobacco (Nicotiana benthamiana) leaf are expressing cytosolic GFP. The brightest region is the nucleus, which is surrounded by a large central vacuole. The cytosol is restricted to the cell periphery. Transvacuolar strands connect the nucleus with other parts of the cell.

Fluorescence microscopy image showing glowing red cells with bright yellow centers and branching extensions against a black background; scale bar indicates 20 µm.

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Ivan Radin @radinbio.bsky.social · 06/05/2026

In case you missed it, the second Plant Imaging Workshop organized by @plantcellatlas.bsky.social is now available on YouTube: www.youtube.com/watch?v=rytH... In this session, you will learn how to get started with MorphoGraphX to do 2D and 3D segmentation. #plantmicroscopy #plantimaging

youtube.com

Imaging Workshop Webinar Series Part 2: Introduction to 2D and 3D Segmentation with MorphoGraphX

YouTube video by Plant Cell Atlas

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Ivan Radin @radinbio.bsky.social · 27/04/2026

This is a section of juvenile moss (Physcomitrium patens) tissue, called protonemata. The apical cells expand by tip-growth, while subapical cells sometimes divide to make lateral filaments. Cytosolic GFP is in green, and chlorophyll autofluorescence is in magenta. #microscopymonday #moss

Fluorescence microscopy image showing a dense network of thin, branching filamentous structures, colored in green and magenta against a black background. The filaments overlap and extend in multiple directions. A scale bar in the lower right corner indicates 250 µm.

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Ivan Radin @radinbio.bsky.social · 06/04/2026

An undergraduate student in the lab, Andy Marchant, made this awesome image of a moss (Physcomitrium patens) apical chloronema cell. This is a beautiful example of how the ER network looks in plant cells (in green). The chlorophyll autofluorescence is in magenta. #microscopymonday #moss

Fluorescence microscopy image showing a green cellular ER network with magenta oval chloroplasts on a black background, with a 3 µm scale bar.

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Plant Cell Atlas @plantcellatlas.bsky.social · 31/03/2026

We are a month away from the second installment of the Plant Cell Atlas Imaging Workshop Series: Introduction to 2D and 3D Segmentation with MorphoGraphX! Don't forget to register! plantcellatlas.org/events

Promotional image for the PCA Imaging Workshop Webinar Series: Introduction to 2D and 3D Segmentation with MorphoGraphX. The promo has a purple background behind text over a green plant cell graphic with a black background. It also includes the details for the webinar including the date/time.

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Plant Cell Atlas @plantcellatlas.bsky.social · 25/03/2026

The Plant Science Art Exhibit is now on display at the University of Minnesota's College of Biological Sciences! This exhibit will be up for the rest of the semester to engage students and inform them about the history and potential of plant science! Learn more about the exhibit: bit.ly/4byqtQo

An art poster of George Washington Carver displayed in a university hallway.

An art poster on Phytoremediation displayed in a university hallway.

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Reposted by Ivan Radin

Tamara Hernández-Verdeja @tamara-hverdeja.bsky.social · 23/03/2026

🌱💚🎉 Sharing (again 😁) my review on regulation of chloroplast biogenesis. First work of my emerging research group at @cbgpmadrid.bsky.social

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Ivan Radin @radinbio.bsky.social · 23/03/2026

I pulled this image from the archives of my PhD work, back when I was working with plant and yeast mitochondria. These are two Arabidopsis guard cells, with their cytosol in magenta, mitochondria in yellow, and chlorophyll autofluorescence in green. #microscopymonday, #plantcells, #guardcells

A fluorescence image showing two curved, kidney-shaped plant guard cells forming a stomatal pore. The cells glow in bright magenta, outlining their overall shape, while clusters of distinct round and irregular structures inside them fluoresce green and yellow. The background is completely black.

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Ivan Radin @radinbio.bsky.social · 09/03/2026

Moss (Physcomitrium patens) protoplasts (wallless cells) transformed with chloroplast envelope protein tagged with mGFP (green). The chlorophyll autofluorescence is in magenta. The protrusions of the chloroplast envelope are called stromules. #microscopymonday, #plantcells, #plantmicroscopy, #moss

Fluorescence microscopy image of a spherical cluster of chloroplasts, showing magenta-stained interiors surrounded by bright green outlines against a black background.

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Ivan Radin @radinbio.bsky.social · 23/02/2026

Surface (epidermal) cells of many plants are shaped like jigsaw puzzle pieces. 🧩 These are epidermal cells of tobacco (Nicotiana benthamiana) expressing cytosolic EGFP. The bright spherical structures are cell nuclei. #microscopymonday, #plantcells, #plantmicroscopy

Fluorescent microscope image showing a dense network of bright green, irregularly shaped plant cells with glowing outlines against a dark background.

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Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 19/02/2026

Registration is NOW OPEN for the second installment of the Plant Cell Atlas Imaging Workshop Series: Introduction to 2D and 3D Segmentation with MorphoGraphX. You can learn more and register here: www.plantcellatlas.org/events

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Ivan Radin @radinbio.bsky.social · 09/02/2026

We were testing lasers and the spectral ability of our confocal (FV3000) on a zoomed-in section of a Brassica rapa leaf. But when we zoomed out, we saw that the lasers we used photobleached chlorophyll autofluorescence in the square area we had zoomed in on. We used a 10x/0.4 objective for this.

Red fluorescence microscopy image showing a dense, mottled red signal across the field with a distinct dark, square-shaped region at the center where fluorescence is absent or reduced; a 100 µm scale bar appears in the lower-right corner.

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Ivan Radin @radinbio.bsky.social · 26/01/2026

These Brewer's yeast cells were in a hurry! This was captured with a point-scanning confocal microscope, which scans one point (pixel) at a time, moving from left to right and top to bottom. As a consequence, cells moving faster than the scan speed appear as streaks or exhibit distortions.

Image shows a gray-scale image of many circular-shaped yeast cells. There are also many streaks caused by fast-moving cells.

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Reposted by Ivan Radin

FocalPlane @focalplane.bsky.social · 16/01/2026

Our featured image shows epidermal cells of a tobacco leaf expressing cytosolic GFP. The image was acquired by Ivan Radin @radinbio.bsky.social. You can read more about the image and Ivan’s research in our post ⤵️ focalplane.biologists.com/2026/01/16/f... #FluorescenceFriday

focalplane.biologists.com

Featured image with Ivan Radin - FocalPlane

Featured image with Ivan Radin - News

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Ivan Radin @radinbio.bsky.social · 06/01/2026

I absolutely love this paper from @stuartmcdaniel.bsky.social It is a part of a must-read list for all new members of my lab. Bryophytes are not early diverging land plants - McDaniel - 2021 - New Phytologist - Wiley Online Library nph.onlinelibrary.wiley.com/doi/10.1111/...

nph.onlinelibrary.wiley.com

Bryophytes are not early diverging land plants

Phylogenetic trees have permeated biology. However, an understanding of how to interpret phylogenies has lagged behind, notably in publications outside of evolutionary biology. Here I argue that some...

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Ivan Radin @radinbio.bsky.social · 05/01/2026

Happy New Year, everyone. For this #microscopymonday, here is a moss (Physcomitrium patens) protoplast (wall-less cell) transformed with a nuclear marker (in magenta). The much brighter area is the nucleolus. The chlorophyll autofluorescence is in green. #moss #plantcells

The image shows a round protoplast filled with green, evenly distributed ovoid-shaped chloroplasts. In the center of the cell is the spherical nucleus in magenta.

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Harmit Singh Malik @harmitmalik.bsky.social · 01/01/2026

Today I made a small but momentous start to work in 2026 by changing a single number. I renamed the file “Papers to write and submit in 2025” to “Papers to write and submit in 2026”. Stay tuned for more file updates on 1st January 2027.

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Reposted by Ivan Radin

Ben Engel @cellarchlab.com · 28/12/2025

EMPIAR-11830 #ChlamyDataset #TeamTomo 🧪 www.cell.com/molecular-ce...

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Reposted by Ivan Radin

Journal of Experimental Botany @jxbotany.bsky.social · 27/12/2025

🔬 EXPERT VIEW 🔬 In this review, Cox & Czymmek cover the recent developments of expansion microscopy techniques in plant systems and provides examples of their applications in plant biology research. 🔗 doi.org/10.1093/jxb/... #PlantScience 🧪 @kcox-bioguy.bsky.social

Fig. 1.Current expansion microscopy (ExM) methods in plant systems and other potential applications. Figure shows a schematic diagram of digested, permeabilized, expanded plant organelles, cells, or tissues embedded in a hydrogel in the center. Left panel showcases ExM techniques successfully applied in plant systems. These include chloroplast and nuclei (isolated organelles), Chlamydomonas, tobacco BY-2 cells, protoplasts, Arabidopsis seed embryos, and Arabidopsis roots. Right panel outlines other potential applications of ExM in other areas of plant biology including developmental biology, plant-microbe interactions, and spatial and single-cell biology. Created in BioRender. Cox, K. (2025) https://BioRender.com/ok72cvy.

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Reposted by Ivan Radin

New Phytologist @newphyt.bsky.social · 28/12/2025

Our #VirtualIssue on Plant terrestrialization focuses on piecing together #streptophyte trait #evolution and curating research that has contributed to advances in the evolutionary inference of (early) land plant form and function 👇 📚 nph.onlinelibrary.wiley.com/doi/toc/10.1... #PlantScience 🧵1/2

Streptophyte terrestrialization and the conquest of land by embryophytes. Illustration adapted from Fig. 1 of de Vries & Archibald (2018; https://doi.org/10.1111/nph.14975) by Debbie Maizels.

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Reposted by Ivan Radin

Journal of Cell Biology @jcb.org · 24/12/2025

A crucial step towards understanding tip growth in plants. Ivan Radin @radinbio.bsky.social (University of Minnesota) highlights work from Ryken et al. of the Bezanilla lab (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/... #CellSignaling #PlantBiology #PlantCellBiology

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Ivan Radin @radinbio.bsky.social · 22/12/2025

These close-ups of mucilage-covered tentacle tips from the leaf of a carnivorous sundew plant (Drosera spatulata) definitely have a strong holiday vibe, so I am posting them just in time for the holidays. Happy Holidays. #microscopymonday #carnivorousplants

The image shows a close-up of the tentacle tip from the leaf of a carnivorous sundew plant (Drosera spatulata). Tentacles are reddish in color and have a thin stalk with a large head at the end, which secretes a drop of clear mucilage that completely surrounds it.

The image shows a close-up of the tentacles from the leaf of a carnivorous sundew plant (Drosera spatulata). One is in the foreground and in focus, while the rest are blurry in the background. Tentacles are reddish in color and have a thin stalk with a large head at the end, which secretes a drop of clear mucilage that completely surrounds it.

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Journal of Cell Biology @jcb.org · 17/12/2025

Spotlight: Ivan Radin discusses new work from Ryken and colleagues (rupress.org/jcb/article/...) which shows how localization and function of autoinhibitory calcium ATPases maintains the strength of the tip-focus Ca2²⁺ gradient during polarized plant growth. rupress.org/jcb/article/... #PlantBiology

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Ivan Radin @radinbio.bsky.social · 15/12/2025

This is what happens to the moss (Physcomitrium patens) chloroplasts when we grow cells on β-lactam antibiotics. β-lactams inhibit the synthesis of the peptidoglycan in the chloroplast envelope, leading to their dramatic expansion. #microscopymonday #moss #plantcells #plantmicroscopy 1/3

The image shows large moss chloroplasts organized in irregular rows. The chloroplasts, in magenta, are somewhat rectangular in shape but with many irregularities. Many green tubular protrusions extend from the chloroplast surface.

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Ivan Radin @radinbio.bsky.social · 01/12/2025

The Arabidopsis thaliana cells can have different types of plastids. In this image, plastids are labeled with the stroma-targeted fluorescence marker RecARed. RecARed fluorescence is false-colored yellow/orange, while the chlorophyll autofluorescence is in grey. #microscopymonday #plantcells

The image shows two types of plastids, bigger ones with an ovoid shape in grey, and much smaller ones with more complex structures in yellow/orange. The smaller ones also have many filamentous structures expanding from them.

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Ivan Radin @radinbio.bsky.social · 10/11/2025

During my postdoc, I looked at hundreds of images like this 🤩 These are two apical caulonemal cells from moss Physcomitrium patens stained with MDY64 (shown in shades of orange). The natural autofluorescence of chlorophyll is in cyan. #microscopymonday #moss #plantcells #plantmicroscopy

Image shows two elongated cells with rounded tips. The cell periphery and internal structures are visible in different shades of orange. Some internal structures are very small, while others are very big and occupy a large part of the cell. Small cyan-colored ovoid chloroplasts are dotted around within the cells.

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Ivan Radin @radinbio.bsky.social · 27/10/2025

In preparation for Halloween, these chloroplasts had a seance! 👻🎃 These five Arabidopsis chloroplasts were extending their stromules (protrusions of the chloroplast envelope and stroma) towards each other. #microscopymonday #Arabidopsis #plantcells #plantmicroscopy

The image shows several chloroplasts in green and magenta or both. In the center of the image, five chloroplasts are organized in a circle, and each is making a protrusion towards the center of the circle. The center of these chloroplasts is in magenta, while the periphery and the protrusions are in green.

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Reposted by Ivan Radin

Nick Desnoyer @nickdesnoyer.bsky.social · 27/10/2025

Genetically engineered color-changing Arabidopsis 🧬📷- attempt #3 I think I finally nailed it with this one.

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Ivan Radin @radinbio.bsky.social · 20/10/2025

This image of moss Physcomitrium patens beautifully demonstrates what we mean when we say that this cell type is characterized by oblique cell walls. You can see that the cell walls between cells are at an angle and not perpendicular to the cell axis. #microscopymonday #moss #plantcells

Image shows a filament of three elongated moss cells. The cell outline is in cyan, due to the cell wall staining, while the cell interior appears mostly empty apart from the small and numerous ovoid-shaped chloroplasts in magenta.

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Ivan Radin @radinbio.bsky.social · 13/10/2025

Here are some “leaf” or phyllid cells from moss Physcomitrium patens gametophore. Cells are expressing a green fluorescence marker that labels the vacuolar membrane. Chloroplasts' autofluorescence is in magenta. Can you spot dividing chloroplasts? #microscopymonday #moss #plantcells

The image shows several elongated rhomboid-shaped cells, filled with ovoid chrooroplasts in magenta, as well as a network of bubble-like vacuoles.

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Liz Haswell @ehaswell.bsky.social · 07/10/2025

I started to work on MscS-Like proteins 20 years ago, dreaming that they were involved in plant mechanotransduction. And it is becoming more & more clear that they are! #plantscience 🧪 "DmMSL10 is crucial for mechanosensing, facilitating AP firing by generating a receptor potential (RP) amplitude."

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eLife @elife.bsky.social · 08/10/2025

Inside a thylakoid membrane The molecular architecture of the thylakoid membrane in a vascular plant has been determined with single-molecule precision. buff.ly/U9TzrOh

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Ivan Radin @radinbio.bsky.social · 06/10/2025

This is a section of the cotyledon (first leaf) surface from the flowering plant Arabidopsis thaliana, which is expressing the green fluorescent protein vacuolar membrane marker (vac-GB). Chloroplasts are in magenta, visible due to the autofluorescence of the chlorophyll. #microscopymonday

The image shows epidermal Arabidopsis cells (shaped like puzzle pieces) outlined by green lines. Some cells have bright green within them. Ovoid-shaped chloroplasts in magenta are visible throughout the image.

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Ivan Radin @radinbio.bsky.social · 01/10/2025

In case you missed it, our workshop on Fiji Basics for Visualization and Quantification of Plant Images is now available on YouTube: www.youtube.com/watch?v=4TMJ... We give a lot of practical tips on how to get started with Fiji. Keep an eye out for announcements on follow-up workshops.

youtube.com

Imaging Workshop Webinar Series: FIJI Basics for Visualizing and Quantifying Plant Images

YouTube video by Plant Cell Atlas

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Reposted by Ivan Radin

Plant Cell Atlas @plantcellatlas.bsky.social · 22/09/2025

In Two Days! Attendees: we encourage you to download and install FIJI in advance of the workshop if you wish to follow along on your own computer. Keep an eye on your email & visit: hpc.nih.gov/apps/Fiji and fiji.sc Register Now! bit.ly/45zJE7V

Promotional image for the PCA Imaging Workshop Webinar Series: FIJI Basics for Visualizing and Quantifying Plant Images. The promo has a purple and green plant cell graphic with a black background. It also includes the details for the webinar including the date/time: Wednesday September 24, 2025 9:30am CT/10:30am ET/7:30am PT, the Zoom Link, and the Organizers: Ivan Radin, Kirk Czymmek, and Daniel Kierzkowski.

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Ivan Radin @radinbio.bsky.social · 22/09/2025

This is what happens when you remove the wall from plant cells. They become perfectly spherical and are called protoplasts. These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey. #microscopy

The image shows many perfectly spherical cells (protoplasts). All of them are filled with numerous oval-shaped chloroplasts in grey. Some also have orange signal filling either the whole sphere or only part of it.

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Ivan Radin @radinbio.bsky.social · 20/09/2025

Our Plant and Microbial Biology Graduate Program (cbs.umn.edu/pmb/graduate...) is organizing two Zoom Admissions open houses where you can learn about preparing a strong application and get advice about graduate school from current PMB students. Drop in at any point on either or both days, see below

cbs.umn.edu

Plant and Microbial Biology Graduate Program | College of Biological Sciences

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Ivan Radin @radinbio.bsky.social · 15/09/2025

Do you know what happens when you touch a carnivorous sundew plant? If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.

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Ivan Radin @radinbio.bsky.social · 08/09/2025

This might be one of the simplest images I captured, but very fascinating nonetheless, as it shows how tight the cytoplasm in a plant cell is. The cytosol and all other organelles are squished between the plasma membrane (magenta) and the central vacuole (its membrane is green) #MicroscopyMonday

The image shows one straight magenta line and, close to it, another wavy green line.

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Ivan Radin @radinbio.bsky.social · 01/09/2025

We love working with our moss, Physcomitrium (formerly Physcomitrella) patens. In this dissecting microscope image, you can see the juvenile protonema filaments spread away from the main plant as they explore the new areas. #MicroscopyMonday #Moss #Plants

The image shows four moss plants placed on a 2 by 2 grid. Each plant has a dark green, dense center and light green branching filaments that grow away from the main plant.

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Ivan Radin @radinbio.bsky.social · 25/08/2025

Did you know that plants can have different types of plastids/chloroplasts? The small green plastids are from the surface (epidermal) cells of the Arabidopsis leaf, while the much bigger magenta plastids are from the deeper spongy tissue. See video below. #MicroscopyMonday #cellbiology #plastids

The image shows two types of leaf plastids, the larger magenta ones are clustered in large potato-shaped cells. The smaller plastids are green and distributed all over the image. Many of them have small thread-like protrusions.

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Universität Münster @uni-muenster.de · 22/08/2025

About 250 scientists from all over the world will meet at the University of Münster from 24 to 29 August for the "Chlamy 2025" conference to discuss the latest research findings on microalgae.

uni-muenster.de

A focus on the microalgae ‘Chlamy’

About 250 scientists from all over the world will meet at the University of Münster from 24 to 29 August for the "Chlamy 2025" conference to discuss the latest research findings on microalgae.

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