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Rachel Silverstein

@rachelsilverstein9.bsky.social

40 followers 12 following 0 posts

Harvard BBS grad student Kleinstiver lab ✂️🧬

Posts Replies Media

Reposted by Rachel Silverstein

Doudna Lab @doudna-lab.bsky.social · 23/04/2025

Now online at Molecular Cell! What makes SpyCas9 such an efficient editor? Read more at: www.cell.com/molecular-ce... Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!

Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing

A two-step target capture process—specific but low-affinity PAM binding followed by rapid DNA unwinding—underpins efficient CRISPR-Cas9 editing. Shi et al. show that PAM-relaxed Cas9 variants like SpR...

Reposted by Rachel Silverstein

Ben Kleinstiver @bkleinstiver.bsky.social · 22/04/2025

In @nature.com we describe the use of scalable #proteinengineering & #machinelearning to predict millions of bespoke CRISPR enzymes, offering safer & more efficient genome editing tools 🧬🖥️ @rachelsilverstein9.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social www.nature.com/articles/s41...

Custom CRISPR—Cas9 PAM variants via scalable engineering and machine learning - Nature

Nature - Custom CRISPR—Cas9 PAM variants via scalable engineering and machine learning