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Philipp Lange (he/him) @philipplange.bsky.social · 31/08/2026

Enjoy the preprint and stay tuned for more patient SCP data from the Bruker timsTOF ultra and Thermo Fisher Scientific Astral Zoom coming soon.

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Philipp Lange (he/him) @philipplange.bsky.social · 31/08/2026

In this first study we only looked at a small number of participants and limited number of cells but it already indicates not only the feasibility of SCP in these tiny cells but also the huge potential for future discoveries.

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Philipp Lange (he/him) @philipplange.bsky.social · 31/08/2026

We then went on to map these populations to evaluate their morphology and localization in bone marrow biopsies and found signs of differences in stem like phenotypes, and more, across SCP, IHC and histology.

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Philipp Lange (he/him) @philipplange.bsky.social · 31/08/2026

Farah and team (Felix Kraus, Agustina Conrrero, Jonathan Bush,Audi Setiadi) analyzed single cells from 3 kids with B-ALL and 2 non cancer control participants and found that SCP finds leukemic subpopulations not apparent by clinical flow cytometry.

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Philipp Lange (he/him) @philipplange.bsky.social · 31/08/2026

Studying leukemic B-cells from patients by MS is not easy - very small (~1/5th of HELA) with <50pg protein. PhD student Farah Jayousi took this challenge on, optimized method to quant ~1,000 proteins in single primary patient B-ALL cells (>2,500 in HELA) after filtering on Bruker timsTOF ultra.

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Philipp Lange (he/him) @philipplange.bsky.social · 31/08/2026

New preprint form the lab - Single-Cell proteomics discerns patient-specific subpopulations in pediatric B-cell acute lymphoblastic leukemia www.biorxiv.org/content/10.6...

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Nikolai Slavov @slavov-n.bsky.social · 05/04/2025

The JPR issue on Single-Cell Omics is out. As the editorial highlights, understanding biological systems requires direct measurements at all molecular levels. Unlocking the Potential of Single-Cell Omics pubs.acs.org/doi/10.1021/...

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Philipp Lange (he/him) @philipplange.bsky.social · 03/04/2025

Thank you CBC for covering our latest study using #proteomics and rapid patient derived models to inform patient care - out in EMBO Molecular Medicine. A first in Canada step towards improved personalized #PrecisionMedicine CBC: www.cbc.ca/player/play/... Article: www.embopress.org/doi/full/10....

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Philipp Lange (he/him) @philipplange.bsky.social · 01/04/2025

Our first case-study using hashtag#proteomics and rapid patient derived models in parallel to genome sequencing to inform patient care now out in EMBO Molecular Medicine. lnkd.in/gc3AKEfR

lnkd.in

This link will take you to a page that’s not on LinkedIn

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Philipp Lange (he/him) @philipplange.bsky.social · 01/12/2024

Classics. Quick reads that will get you off to a good start.

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Philipp Lange (he/him) @philipplange.bsky.social · 25/11/2024

Next batch has been added to the proteomics starter pack. go.bsky.app/GYoxs2r

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Philipp Lange (he/him) @philipplange.bsky.social · 23/11/2024

Thanks for listing me! Appreciate it. Can you please also add me to the SCP pack. We are far from where we want to be but our data on cells from kids with leukemia is already blowing my mind.

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Philipp Lange (he/him) @philipplange.bsky.social · 22/11/2024

Done. I thought I had you already on there - must have been dreaming.

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Philipp Lange (he/him) @philipplange.bsky.social · 22/11/2024

To keep the conversations going I created a starter pack for proteomics in Canada Let me know if I missed you and I will continue to add as people gather under the blue sky. go.bsky.app/PUWNxFP

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Philipp Lange (he/him) @philipplange.bsky.social · 22/11/2024

The growth of this community is amazing. I started a starter pack to bing the proteomics community together and accelerate this further Proteomics - go.bsky.app/GYoxs2r Please let me know if I missed you. I will curate this pack as new people gather under the blue sky #proteomics #team-massspec

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Philipp Lange (he/him) @philipplange.bsky.social · 22/11/2024

It has been so much fun to run this. The science was outstanding and the connections that were made will be long lasting. We had it all - breakthrough science, bears guarding the pub and the first snow of the season. Thank you all for coming! See you at HUPO/CNPN 2025 in Toronto.

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Philipp Lange (he/him) @philipplange.bsky.social · 19/11/2024

Next up: Dr Michael Lassman from Merck giving the 2nd Keynote at #CNPN2024.

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Reposted by Philipp Lange (he/him)

Thibault Mayor @cellsandprots.bsky.social · 18/11/2024

Ryan from our lab presenting his work @cnpn looking at yeast strains capable of producing more recombinant proteins. When we started doing #proteomics we actually found quite important differences between these strains More here www.biorxiv.org/content/10.1...

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Reposted by Philipp Lange (he/him)

Thibault Mayor @cellsandprots.bsky.social · 18/11/2024

Snowflakes & Proteomics: the 2024 CNPN Fairmont fairytale… once upon a time there was a tiny single cell (Ljijana Pasa-Tolic), a mutated proteome (Matthew Berg from the Villen lab) and a nasty protease (Olivier Julien)…

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Philipp Lange (he/him) @philipplange.bsky.social · 18/11/2024

It is a pleasure to host this year's conference of the Canadian National Proteomics Network. Such a brilliant and smart crowd that has gathered in Whistler.

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Philipp Lange (he/him) @philipplange.bsky.social · 18/11/2024

This captures our first evening of CNPN 2024 so well!

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Reposted by Philipp Lange (he/him)

CNPN @cnpn-org.bsky.social · 18/11/2024

CNPN 2024 just kicked off by Dr. Mari Demarco and Dr. Philipp Lange in Whistler, BC🥳🙌😍🎉 Thanks so much to our sponsors, especially @Thermofisher

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Reposted by Philipp Lange (he/him)

PastelBio @pastelbio.bsky.social · 24/04/2024

Research Associate I/II, Proteomics – Vividion Therapeutics, Inc – San Diego, CA – San Diego Biotechnology Network sdbn.org/san-diego-b... --- #proteomics #prot-jobs

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Philipp Lange (he/him) @philipplange.bsky.social · 17/04/2024

"Budget 2024 proposes to provide $825 million over five years, starting in 2024-25, with $199.8 million per year ongoing, to increase the annual value of master's and doctoral student scholarships to $27,000 and $40,000, respectively, and post-doctoral fellowships to $70,000."

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Philipp Lange (he/him) @philipplange.bsky.social · 17/04/2024

Budget day and A fantastic day for 🇨🇦 Canadian science! Finally students and postdocs get recognized for their outsized contributions to society and see big increases in fellowships. Great time to join a 🇨🇦 lab! Reach out.

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Philipp Lange (he/him) @philipplange.bsky.social · 21/02/2024

@lkpino.bsky.social @talusbio.bsky.social kicking off the BC Proteomics & Metabolomics Network Symposium with a phenomenal keynote. Transcription factors, mass spec in drug development, startups, off target effects, … beautiful tour de force. #proteomics.sky

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Philipp Lange (he/him) @philipplange.bsky.social · 21/02/2024

I am nevertheless glad you made it! Welcome.

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Philipp Lange (he/him) @philipplange.bsky.social · 21/02/2024

Sadly true by now but just a quick hop over to France and you zip away.

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Philipp Lange (he/him) @philipplange.bsky.social · 20/02/2024

Interested in relocating to beautiful Vancouver? Feel free to DM / email with more specific interests / goals and we can see if there is something we can make happen.

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Philipp Lange (he/him) @philipplange.bsky.social · 12/02/2024

Do you know how rayzen and threadripper compare. Rayzen tend to have faster single core speeds but fewer threads.

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Philipp Lange (he/him) @philipplange.bsky.social · 11/02/2024

Is disk speed still relevant or is it really mostly memory speed/capacity and threads? How relevant is single core speed these days?(ie how many key steps are not parallelized?)

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Philipp Lange (he/him) @philipplange.bsky.social · 11/02/2024

So GPUs ore out. Great, one component less to optimize.

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Philipp Lange (he/him) @philipplange.bsky.social · 11/02/2024

I am considering to bring fragpipe/diann to the Canadian HPC grid. Just need to find the time.

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Philipp Lange (he/him) @philipplange.bsky.social · 11/02/2024

But even if you get it to run on Apple Silicon the box around it drives up the cost likely making a cluster the cheaper option.

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Philipp Lange (he/him) @philipplange.bsky.social · 08/02/2024

That is good to know. Not sure where I got the idea. So I guess it’s still the same then. High thread count, high single core speed and lots of RAM? Other than for Maxquant that (used to) read/write many small temp files disk speed is not a major factor, correct?

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Philipp Lange (he/him) @philipplange.bsky.social · 08/02/2024

Thanks. Yes, let's talk! The BCPM Symposium in Vancouver will be a good opportunity to chat in person. Thanks for accepting our invitation to give the Keynote!

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Philipp Lange (he/him) @philipplange.bsky.social · 08/02/2024

What are people's current preferred configurations for systems running Spectronaut or DIANN? Do both make use of GPUs? #proteomics #TeamMassSpec

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Reposted by Philipp Lange (he/him)

Ben Neely @benneely.com · 31/01/2024

POTRHList, why not! Thanks to @ucdproteomics.bsky.social and all the people who joined. Some random links to topics. 1/n

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Philipp Lange (he/him) @philipplange.bsky.social · 31/01/2024

First timsTOF Ultra in Canada🇨🇦 installed (Dec 2023) @BC Children's & @ubcmedicine.bsky.social. We were busy with our first neuroblastoma cohort so forgot to share this exciting news here. Heartfelt thanks to our donors & BC Children's Hospital Foundation! #TeamMassSpec #Proteomics #Cancer

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Philipp Lange (he/him) @philipplange.bsky.social · 20/01/2024

How about we classify every grant chatGPT can come up with as incremental, obvious next step. We allocate some 30% of funding to get these things done in automated AI driven labs and allocate the rest to projects no AI but only humans can hallucinate. Could do some serious cool stuff!

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Reposted by Philipp Lange (he/him)

Lindsay K Pino @lindsaykpino.com · 19/01/2024

I'm team-teaching with Brian Searle at the Ubuntu School in a couple weeks. We're putting together a whole new set of lectures and tutorials for data quality assessment of DIA and other chromatogram-based quantitative mass spec using the @benneely.com ABRF sPRG tribrid proteome as demo data

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Philipp Lange (he/him) @philipplange.bsky.social · 19/01/2024

Would a term describing the range of input material work, e.g nano-proteomics (ng inputs) or X (Roman for ten cells) or 100squared (if you go by area)

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Philipp Lange (he/him) @philipplange.bsky.social · 19/01/2024

Can you define it by what it is rather than the amount of protein you get. Eg defined cell population, proteome subset, …

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Reposted by Philipp Lange (he/him)

Sara Gosline (she/her) @sargoshoe.bsky.social · 17/01/2024

Excited to introduce this behemoth of a manuscript enabling the prediction of drug response in acute myeloid leukemia through integration of protein and gene measurements across hundreds of patient samples. #AML #drugresistance #machinelearning #proteomics www.cell.com/cell-reports...

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Eric J. Perkins, PhD (he/him) @allostericsite.bsky.social · 11/01/2024

Hey, science folks of Bluesky! Addgene has announced that it is fully committing to leaving X. They are taking a hit in their social media visibility to do this, but they have good reasons (blog.addgene.org/addgene-is-s...), as many of us do. Please give them a follow: @addgene.bsky.social

blog.addgene.org

Addgene is Stopping Use of X (formerly Twitter)

Addgene will no longer be posting, monitoring, or responding to posts on X. You can find us as @addgene.bsky.social on Bluesky or as @addgene on Instagram, TikTok, Facebook, and LinkedIn.

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