Osvaldo Burastero @osvalb.bsky.social · 10/06/2026Moreover, the errors of the fitted parameters can be easily estimated through leave-one-out or profile likelihood analysis (thank you reviewer #3) 😁 100 0
Osvaldo Burastero @osvalb.bsky.social · 10/06/2026And fitted (both 330 nm and 350 nm) with a global model to obtain estimates of ΔCp, ΔHm, Tm and the m-value. 100 0
Osvaldo Burastero @osvalb.bsky.social · 10/06/2026CheMelt expands the work done by Hamborg et al., (2020). Global analysis of protein stability by temperature and chemical denaturation. Analytical biochemistry. For example, they kindly provide an unfolding dataset of the ACBP protein which can be imported in CheMelt: 100 0
Osvaldo Burastero @osvalb.bsky.social · 10/06/2026Do you want to study the stability of a designed protein that doesn't unfold below 90 ºC 🔥? Combine a denaturant and temperature and then analyse the results with our newest tool: CheMelt 🧪 Online: - spc.embl-hamburg.de/app/chemelt As Docker image (faster): - hub.docker.com/r/emblspc/ch... 111 0
Osvaldo Burastero @osvalb.bsky.social · 30/03/2026Interested in protein biophysics?🧪 Sign up for the @springernature.com webinar (08/04) where we will present the MOSBRI project outcomes @mosbri.bsky.social, including the eSPC platform (spc.embl-hamburg.de) and the Molecular Biophysics Database (mbdb-data.org) 🙌 Info here: tinyurl.com/rdfsp4nv 110 0
Osvaldo Burastero @osvalb.bsky.social · 19/02/2026Do you have to create a scientific poster? 🧪 I have created a basic Figma template for it. Hope it helps 👐: github.com/osvalB/figma... 010 0
Osvaldo Burastero @osvalb.bsky.social · 13/08/2025Do you want to analyze mass photometry (MP) count data with Python scripts? Try pyPhotoMol, a package to process, fit, and visualize the MP data 👨💻 github.com/osvalB/pypho... pyPhotoMol is derived from PhotoMol, our web tool for MP: spc.embl-hamburg.de/app/photoMol #massphotometry #Python #code 063 0
Osvaldo Burastero @osvalb.bsky.social · 14/02/2025Interested in antimicrobial resistance and drug discovery? Attend the talk of my colleague and partner Tania Szal 😊 Monday 17th at 8:30. Platform: protein-small molecule interactions I #BPS2025 010 0
Osvaldo Burastero @osvalb.bsky.social · 14/02/2025Looking forward to making new connections at #BPS2025👨 Meet me at poster B38 on Tuesday 18 Feb 13:45. Session "protein stability folding and chaperones" 031 0
Osvaldo Burastero @osvalb.bsky.social · 11/02/2025Just finished a science poster (in Figma) and wanted to share the template I used! 🎨 Hope it's useful: github.com/osvalB/figma... 020 0
Osvaldo Burastero @osvalb.bsky.social · 31/01/2025The estimated Kd (micromolar) is 1.3, and we also provide the asymmetric confidence interval: [0.5 - 3.1] (link.springer.com/article/10.1...) The reported Kd by the authors was 1.2. 6. Extra: I scaled the plot to start around 0. 000 0
Osvaldo Burastero @osvalb.bsky.social · 31/01/20254. Go to the '2. Fitting' section. Inside the 'Advanced settings', set the Kd max value to 5 micromolar (to help the fitting algorithm 😄😬 ). 5. Press on 'Run fitting' and voila! 110 0
Osvaldo Burastero @osvalb.bsky.social · 31/01/20252. Import the file into our tool ThermoAffinity (spc.embl-hamburg.de/app/thermoAf...) 3. Set the units to 'Nanomolar' and the hot region between 14 - 20 seconds (based on what the authors reported) 110 0
Osvaldo Burastero @osvalb.bsky.social · 29/01/2025Are you working with circular dichroism (CD)? Check out the latest update to our ChiraKit tool, now with 10 tutorials to get you started! 🚀 Try it here: spc.embl-hamburg.de/app/chirakit 020 0
Osvaldo Burastero @osvalb.bsky.social · 18/11/2024Just out! 🥳🥳 Great work done at the @spcembl.bsky.social to understand clathrin-mediated endocytosis. We investigated how clathrin and adaptor proteins interact in vitro and in vivo. Kudos to the 1st author @lucasdefelipe.bsky.social! www.nature.com/articles/s41... www.embl.org/news/science... 1117 0
