This Primer highlights micropore design principles governed by distinct separation mechanisms to resolve key performance trade-offs in industrial gas separations. It outlines the experimental workflow...

Polyoxometalates (POMs) are anionic molecular metal oxide clusters composed of high-valent early transition metals. In this Primer, Anjass et al. describe how redox-active POMs are selected, synt...

Optical photothermal infrared spectroscopy is capable of submicrometre infrared analysis with minimal water interference and scattering artefacts. This indirect, non-contact pump–probe method circumve...

A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

This Collection features Primers, PrimeViews, Comments, and a Q&A on spectroscopy and microscopy methods.

Long-read sequencing facilitates observation of contiguous DNA molecules, providing access to variants previously ignored. In this Primer, Zheng et al. discuss the computational foundations of long-re...

Imaging mass cytometry is a highly multiplexed tissue-imaging technique that allows the simultaneous in situ detection of up to 45 markers with subcellular-level resolution. In this Primer, Meyer et a...

High-temperature electrochemical devices operate at gas–solid interfaces in highly redox-active environments. In situ and operando characterization techniques are crucial for monitoring the structural...

Advances in electron microscopy instrumentation and methods continue to drive progress in the physical and life sciences. The 4th edition of this conference will bring together researchers from physic...

A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

This Primer provides an introduction to positron annihilation spectroscopy and its uses in studying atomic-scale structures in solids.

Optimizing Raman spectrometer designs for use in extreme environments can enable acquisition of critical information that may not be accessible in situ by humans. Technology advancements currently bei...

Photoacoustic tomography (PAT) is a biomedical imaging technique based on the use of optically excited ultrasound waves. In this Primer, Guggenheim et al. describe the noise-equivalent pressures repor...

Neutron spin echo spectroscopy investigates internal dynamics and diffusion in materials, using advanced instruments and data collection techniques. In this Primer, Faraone et al. discuss how neutron ...

Peptides have become an increasingly important and versatile ligand type for G protein-coupled receptors (GPCRs). In this Primer, Hermes et al. discuss the experimental and computational workflow...

Distributional regression models entire conditional response distributions. In this Primer, Merder et al. discuss how distributional regression is used to predict uncertainty and extreme conditions in...

Conductive polymers (CPs) are π-conjugated organic semiconductors that exhibit electrical conductivities similar to insulators and metals while maintaining the flexibility of plastics. This Primer pro...

Solid-state laser cooling is a technique used to lower the temperature of a condensed-phase system through its interaction with light. In this Primer, Ding et al. discuss the fundamental principles of...

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a label-free molecular imaging approach mapping diverse biomolecules at cellular resolution. In this Primer, Spragg...

Cucurbit[n]urils are macrocyclic host molecules that form strong complexes with hydrophobic and cationic guests in water, where n is the number of glycoluril units. Cucurbit[n]urils complexed with gue...

Enzyme-tethering epigenomic methods modify DNA while cells and nuclei remain intact, making them particularly suitable for single-cell and spatial applications. In this Primer, Kami Ahmad et al. discu...

Atomic-scale engineering of MgO-based magnetic tunnel junctions enables coherent spin-dependent tunnelling and large tunnelling magnetoresistance for advanced spintronic functionality. This Primer out...

Atoms and molecules are the fundamental building blocks of matter. Spectroscopy identifies and quantifies chemical species through the unique spectral fingerprints they imprint on light. Spectroscopy ...

Dual-comb spectroscopy utilizes two optical frequency combs for precise, rapid measurements, enhancing performance through time-domain interferometry. This Primer discusses the experimental set-up and...

Transient absorption spectroscopy is a pump–probe method used to investigate light-induced dynamics. In this Primer, He et al. describe various transient absorption set-ups and outline a workflow for ...

Flow NMR spectroscopy offers real-time monitoring of chemical reactions, enabling quantitative analysis and mechanistic insights. This Primer describes the specialized equipment and tailored methods t...

This Primer provides a comprehensive overview of laser Doppler vibrometry, covering fundamental principles, advanced measurement methods and diverse applications. It highlights performance limits, mea...

A celebration of the striking and colourful work that scientists do around the world.

Expansion microscopy enhances conventional imaging by physically enlarging samples within a hydrogel matrix, surpassing diffraction limits. In this Primer, Vo et al. detail typical expansion microscop...

Current nanoherbicide development is largely guided by a material-first design logic that overlooks the biological complexity determining nanoparticle transport, transformation and target-site access in plants. We propose plant-informed nanodesign; a biology-driven framework that integrates plant physiology into rational nanocarrier engineering, advancing the field from empirical screening towards predictive science.

Base editing enables precise, programmable single-base changes using cytosine or adenine editors. This Primer outlines their use in mammalian cells for screening, disease modelling and therapy, covering experimental design, analysis, limitations and best-practice guidance to support effective implementation.

Magnetoresistance is a fundamental transport phenomenon that provides deep insight into electronic structure, magnetic order and emergent quantum effects in solids. This Primer reviews major magnetore...

This ongoing Series of ‘How to’ Comments offers expert guidance and recommendations on a wide range of topics relevant to the broad readership of Nature Human ...

Ultrafast near-field microscopy unites femtosecond optical spectroscopy with nanometre spatial resolution to image non-equilibrium material dynamics beyond the diffraction limit. This Primer outlines ...

This Primer introduces hyperspectral imaging (HSI) through a concise, imaging-centric perspective, linking sensor platforms, data types and representative datasets across application domains. It highl...

This Primer provides an introduction to quantum imaging with correlated photon pairs, from core concepts to experimental realizations. It discusses potential advantages over classical imaging, practic...

Fluidic force microscopy combines atomic force microscopy with microfluidic probes to enable measurement and manipulation of materials at sub-micrometre resolution. In this Primer, Zambelli et al. dis...

Advancements in artificial intelligence and machine learning are reshaping scientific research, with notable benefits for materials design and modelling. We spoke with Aron Walsh, Professor in Materia...

Electrophoretic deposition is a micropatterning method that utilizes suspension properties and kinetic parameters for film control. This Primer discusses innovative electrode design to enhance resolut...

Clear, consistent communication is essential in science and a goal Nature Reviews Methods Primers addresses with the ‘Reproducibility and data deposition’ section in Primers. Here, Brynn Hibbert of th...

The UK Reproducibility Network (UKRN) was established to promote robust and transparent research and drive research quality. Here, Marcus Munafò, a founding member of the UKRN, outlines the role of Re...

With the rising success of solar photovoltaics, long-term sustainability needs to be addressed. We provide practical steps that researchers, journals and the wider community can take to embed circular...

Artificial intelligence is increasingly used for data analysis, literature synthesis and experimental design, posing a challenge to open research. Here, David Moher of the Ottawa Hospital Research Ins...

Optimizing Raman spectrometer designs for use in extreme environments can enable acquisition of critical information that may not be accessible in situ by humans. Technology advancements currently bei...